RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs

Garcia, Denise K.; Benzie, John A. H.

doi:10.1016/0044-8486(94)00311-b

Cited by 52 publications

(26 citation statements)

References 12 publications

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“…However, there are also caveats with this technique, particularly with regard to reproducibility issue (reviewed by Ali et al, 2004). The technique has been successfully used to characterize the genetics of crustacean such as Macrobrachium borelli (D'Amato & Corach, 1996) and Penaeus monodon (Garcia & Benzie, 1995).…”

Section: Introductionmentioning

confidence: 99%

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii

Imron¹,

Sunandar²,

Susilowati³

et al. 2009

Indonesian Aquaculture Journal

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Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.

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Section: Introductionmentioning

confidence: 99%

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii

Imron¹,

Sunandar²,

Susilowati³

et al. 2009

Indonesian Aquaculture Journal

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“…It has been successfully employed to determine genetic diversity in Litopenaeus vannamei, (Boone, 1931) (Garcia and Benzie, 1995), and P. monodon, (Tassannakajon et al, 1998) for population studies of Penaeid species. In Thailand found Banana prawns F. merguiensis and F. indicus are clearly distinguished by RAPD marker (Phongdara et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Economically Important Penaeid Population in South East Coast of India

Rajakumaran

Vaseeharan²,

Yeshvadha³

2013

ija

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Genetic variation of wild Penaeid population of South East Coast region in India, using Randomly Amplified Polymorphic DNA (RAPD) analysis as DNA marker has been examined. Important penaeid species like Fenneropenaeus indicus, Penaeus semisulcatus, Penaeus monodon were collected at different geographical location such as Chennai, Nagapattinam, Pudukkottai, Ramanathapuram and Tuticorin, but P. monodon was not obtained from Tuticorin. For RAPD analysis, 15 numbers of primers were used, among which 3, 4, and 6 numbers of primers have worked well for F. indicus, P. semisulcatus and P. monodon respectively. The results of this study, genetic variation (6-33)% for F. indicus, (16-49)% for P. semisulcatus (16-53)% for P. monodon. As conclusion, from the pattern of genetic diversity of these three penaeid population, it could be considered as moderate to high, and maximum three population stocks are within the collected area.

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“…Information of the genetic moleculer level is very important to study about identification of species, breeding programs, phylogenetic study, genetic variation and preservation of genetic diversity [9,10]. Diversity analyses require a large number of polymorphic markers to measure genetic relationships and genetic diversity in a reliable manner [11].…”

Section: Introduction Il Palm (E Guineensis ) Is a Species From Fmentioning

confidence: 99%

Identication of Variance Moleculer Genotype Commercial Oil Palm (Elaeis guineensis Jacq.) based on Random Amplified Polymorpism DNA (RAPD) Markers

Pasaribu

Putri

Suryanto

2017

ins

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-Commercial oil palm is one of plant genotype have a inbreeding so that it will be genetic depression. Genetic depression can cause difficulties to identification of genetic trait in palm oil, so that identification of moleculer variance is necessary to know the genetic potential of commercial palm oil to obtain initial information about genetic diversity. The objectives of this study was to find genetic diversity in commercial oil palm (E. guineensis Jacq.) by using five RAPD primers . The results of this study showed that Polymorpism Information Content (PIC) was 0.338. PCoA analysis showed that the moleculer varian was 43,72% and according to profil radial neighbour joining tree (NJtree) showed that three main groups.

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RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs

Cited by 52 publications

References 12 publications

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii

Molecular Characterization of Economically Important Penaeid Population in South East Coast of India

Identication of Variance Moleculer Genotype Commercial Oil Palm (Elaeis guineensis Jacq.) based on Random Amplified Polymorpism DNA (RAPD) Markers

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