RAPD-PCR investigations of systematic relationships among four species of eels (Teleostei: Anguillidae), particularly Anguilla anguilla and A. rostrata

Lehmann, Debra; Hettwer, H.; Taraschewski, Horst

doi:10.1007/s002270000349

Cited by 26 publications

(9 citation statements)

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“…1997; Nieddu et al. 1998; Lehmann et al. 2000) proved to distinguish A. rostrata from A. anguilla readily, thus confirming earlier suspicions based on morphology that two mostly separate gene pools of eels co‐reside in the North Atlantic Basin.…”

Section: History Of Indirect Mating Evidence From Continental Samplessupporting

confidence: 82%

Catadromous eels continue to be slippery research subjects

Avise¹

2011

Molecular Ecology

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As adults, Atlantic eels (Anguilla rostrata in the Americas and Anguilla anguilla in Europe) are tubular slimecovered fish that spend most of their catadromous life cycle in coastal environs before swimming far out to sea to reproduce, as part of an intergenerational migratory circuit that provides an interesting reversal of the pattern displayed by adult anadromous salmon that live mostly in the ocean but then migrate long distances to spawn in freshwater streams. Earlier genetic findings on Atlantic eels involved specimens collected across their broad continental ranges and generally indicated that conspecifics probably engage in panmictic or quasi-panmictic spawning, from which arise leaf-shaped leptocephaus larvae that then disperse back to coastal locations more or less at random with respect to the widespread geographical origins of the parental genes they carry. In this issue, Als et al. (2011) add exciting information about this peculiar life-history pattern of catadromous Atlantic eels by extending the genetic analyses to eel larvae collected from the Sargasso Sea, the oceanic area where both species spawn. Results help to confirm standard textbook wisdom that these catadromous eels are nearly unique in the biological world by having both broad geographical distributions and yet displaying intraspecific nearpanmixia.

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Section: History Of Indirect Mating Evidence From Continental Samplessupporting

confidence: 82%

Catadromous eels continue to be slippery research subjects

Avise¹

2011

Molecular Ecology

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“…The RAPD±PCR technique has proved to be a highly ecient means to identify and distinguish between morphologically similar species (e.g. Kambhampati et al 1992;Wilkerson et al 1993;Adamson et al 1993;Laroche et al 1996;Smith et al 1996;Schmidt and Westheide 1997/98;AndreÂ et al 1999;Lehmann et al 2000). In the present study, it was used to detect a new meiofaunal species group taxon that could not be revealed by traditional morphological characters of polychaete taxonomy.…”

Section: Discussionmentioning

confidence: 89%

Molecular taxonomy: description of a cryptic Petitia species (Polychaeta: Syllidae) from the island of Mahé (Seychelles, Indian Ocean) using RAPD markers and ITS2 sequences

Westheide

Hass‐Cordes

2001

J Zoological System

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Petitia occulta sp. n. provides an example demonstrating that a morphologically indistinguishable species taxon can be described by molecular markers alone. Phenotypically the polychaete belongs to Petitia amphophthalma Siewing, 1956, one of the numerous meiofaunal species that have previously been regarded as cosmopolitan. Genotypically, however, by means of randomly amplified polymorphic DNA (RAPD)–polymerase chain reaction (PCR) (a DNA‐fingerprinting technique) the population on the Indo‐Pacific island of Mahé can be identified as a distinct taxonomic entity as: (1) genetic distance values between the Petitia from the Seychelles and from other sites (Mediterranean, Canary Islands) are relatively great; (2) differently generated cluster analyses of genetic distances produce phenograms revealing a distinctly separate clade (bootstrap 100%) for the Seychelles animals; and (3) the Seychelles animals are characterized by eight monomorphic diagnostic (i.e. autapomorphic) DNA‐fragments. The RAPD procedure in this case requires the preparation of the whole organisms and, thus, no holotype is available. Instead the RAPD band‐pattern with the diagnostic markers of one individual obtained with six primers, isolated DNA of one individual, and syntypes in the form of fixed, complete individuals are deposited. Species differentiation is confirmed by genomic DNA sequencing (ITS2).

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“…To date, several works exist which use DNA methods for eel identiWcation. Among these methods, techniques such as ampliWcation of a partial sequence of DNA by polymerase chain reaction (PCR) followed by direct sequencing of the ampliWed products [8][9][10], restriction fragment length polymorphism analysis [11][12][13][14][15], single nucleotide polymorphism [16,17], real-time PCR analysis [18,19], microsatellite analysis [20], or randomly ampliWed polymorphic DNA analysis method [13,21,22] are very useful. All these works include a low number of eel species.…”

Section: Introductionmentioning

confidence: 99%

Authentication of the most important species of freshwater eels by means of FINS

Lago

Vieites

Espiñeira

2012

Eur Food Res Technol

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Eels are a taxonomic group with great commercial importance due to their huge steaks. They have very high demand, especially the young ones. There are high morphological similarity and diVerent market values between diVerent species. For these reasons arises the need to develop techniques that allow identifying as many species as possible. In this study, a DNA method based on DNA phylogenetic analysis of sequences (forensically informative nucleotide sequencing) has been developed. This method has been used to authenticate 12 eel species, including the most important to commercial level (Anguilla anguilla, A. rostrata, A. japonica, A. australis), by means of the ampliWcation of a 239-base pair (bp) fragment of the mitochondrial Cytochrome b (cyt b) gene. This method is useful to clarify questions related to the correct labeling of commercial products and to verify the traceability in commercial trade and for Wsheries control.

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RAPD-PCR investigations of systematic relationships among four species of eels (Teleostei: Anguillidae), particularly Anguilla anguilla and A. rostrata

Cited by 26 publications

References 0 publications

Catadromous eels continue to be slippery research subjects

Catadromous eels continue to be slippery research subjects

Molecular taxonomy: description of a cryptic Petitia species (Polychaeta: Syllidae) from the island of Mahé (Seychelles, Indian Ocean) using RAPD markers and ITS2 sequences

Authentication of the most important species of freshwater eels by means of FINS

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RAPD-PCR investigations of systematic relationships among four species of eels (Teleostei: Anguillidae), particularly Anguilla anguilla and A. rostrata

Cited by 26 publications

References 0 publications

Catadromous eels continue to be slippery research subjects

Catadromous eels continue to be slippery research subjects

Molecular taxonomy: description of a cryptic Petitia species (Polychaeta: Syllidae) from the island of Mah&eacute; (Seychelles, Indian Ocean) using RAPD markers and ITS2 sequences

Authentication of the most important species of freshwater eels by means of FINS

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Product

Resources

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Molecular taxonomy: description of a cryptic Petitia species (Polychaeta: Syllidae) from the island of Mahé (Seychelles, Indian Ocean) using RAPD markers and ITS2 sequences