RAPD variation in wild populations of four species of the genus Hordeum (Poaceae)

Bustos, Alfredo de; Casanova, Carlos; Soler, Consuelo; Jouve, N.

doi:10.1007/s001220050715

Cited by 55 publications

(43 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Notwithstanding this species was previously considered to be essentially autogamous, our findings indicate that outbreeding cannot be excluded. This confirms recent results obtained by De Bustos et al (1998) describing at least partial outbreeding in Spanish populations of the same species.…”

Section: Discussionsupporting

confidence: 92%

“…and bearded wheatgrass (Elymus caninus L.), occasionally grow in direct proximity or even within wheat fields and have been described as being not strictly autogamous (Sun et al 1997;De Bustos et al 1998). Several studies have described the production of viable and partially fertile hybrids between these wild species and wheat for agronomic purposes (Sharma and Baezinger 1986;Fedak 1991).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Search for evidence of introgression of wheat (Triticum aestivum L.) traits into sea barley (Hordeum marinum s.str. Huds.) and bearded wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers

Guadagnuolo¹,

Savova-Bianchi²,

Keller-Senften³

et al. 2001

Theor Appl Genet

View full text Add to dashboard Cite

Seeds of English and Austrian populations of bearded wheatgrass (Elymus caninus L.) and sea barley (Hordeum marinum Huds.) growing in the vicinity of wheat (Triticum aestivum L.) fields were collected in order to search for evidence of the introgression of wheat traits into these wild relatives. Seeds were sown and plants grown for subsequent analyses using morphological and genetic (isozymes, RAPD and wheat microsatellites) markers. No F 1 hybrids were found within the individuals of the two species grown, neither with morphological nor with genetic markers. Also, no evidence of introgression of wheat traits into E. caninus was observed. However, in one individual of H. marinum which had the typical morphology of this species, numerous species-specific DNA markers of wheat were amplified, thereby demonstrating previous hybridization. Consequently, the hybridization between wheat and H. marinum under natural conditions and the introgression of wheat traits into this wild relative seems to be possible. Our results contribute to the risk assessment of transgenic wheat cultivation. Keywords Wheat · Wild relative · Gene flow · Introgression · Genetic marker IntroductionAmong the concerns related to genetic engineered crops, the risk of gene escape toward wild flora is one of the most discussed. The potential for such gene flow is directly proportional to the potential of crop-wild hybridization (Ellstrand and Hoffman 1990). For numerous crops, wild relatives are known that can hybridize with them somewhere in the world. This is the case, among others, of maize (Doebley 1990), oilseed rape (Klinger et al. 1992), sunflower (Whitton et al. 1997) and sugar beet (Bartsch and Pohl-Orf 1996).For wheat, the center of distribution is assumed to be the Middle East and the Mediterranean area. Many wild species closely related to wheat exist in these regions; most belong to genus Aegilops and grow close or within wheat fields, frequently hybridizing with this crop (van Slageren 1994). More generally, the Triticeae tribe is distributed worldwide throughout the temperate regions of both hemispheres (Miller 1987). Many wild relatives of wheat have been introduced as adventive in other regions and even on other continents, as is the case for Ae. cylindrica in North America (Donald and Ogg 1991).In northern Europe two wild relatives of wheat, sea barley (Hordeum marinum Huds.) and bearded wheatgrass (Elymus caninus L.), occasionally grow in direct proximity or even within wheat fields and have been described as being not strictly autogamous (Sun et al. 1997;De Bustos et al 1998). Several studies have described the production of viable and partially fertile hybrids between these wild species and wheat for agronomic purposes (Sharma and Baezinger 1986;Fedak 1991). Considering their breeding system, their belonging to the same tribe and the fact that they often grow within a huge amount of wheat pollen, the risk of spontaneous hybridization with wheat cannot be excluded.In order to estimate the ecological risks involved with ...

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Search for evidence of introgression of wheat (Triticum aestivum L.) traits into sea barley (Hordeum marinum s.str. Huds.) and bearded wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers

Guadagnuolo¹,

Savova-Bianchi²,

Keller-Senften³

et al. 2001

Theor Appl Genet

View full text Add to dashboard Cite

show abstract

“…Yang and Quiros (1993) bulked the samples of celery with 10, 20, 30, 40 and 50 individuals and found the same RAPD banding pattern. Bustos et al (1998) also found that bulks of 10 to 20 individuals resulted in the same RAPD profiles. In this study, we used 10 individuals from each sugarbeet population.…”

Section: Discussionmentioning

confidence: 65%

Genetic Diversity Analysis in Sugarbeet (Beta vulgaris L.) Using Isozymes, RAPD and ISSR Markers

et al. 2007

View full text Add to dashboard Cite

Summary Four elite diploid populations of sugarbeet viz. IISR Comp-1, LKC-11, LS-6 and Ramonskaya-06 were examined using isozymes and molecular markers. Seventy-one bands consisting of 28 isomorphs of 6 isozyme systems viz. superoxide dismutase, guaiacol peroxidase, malate dehydrogenase, amylase, esterase and aspartate amino transferase were resolved. Molecular analysis was performed using 28 RAPD (random amplification of polymorphic DNA) and 4 inter simple sequence repeat (ISSR) primers. Highly polymorphic band profiles with 327 RAPD markers (11.67/primer) of 162-2862 bp and 39 ISSR markers (9.75/primer) of 160-1884 bp were obtained. Mean value of Polymorphic Information Content (PIC) for RAPD and ISSR primers was 0.41 and 0.44 respectively. Based on the binary matrix of presence/absence of bands, Dice coefficient of genetic similarity (GS) matrix was computed for each marker system, which ranged from 0.62-0.76 (mean value 0.726), 0.10-0.74 (mean value 0.27) and 0.12-0.86 (mean value 0.35) for isozyme, RAPD and ISSR markers. There was significant correlation (rϭ0.908) between the RAPD-GS and ISSR-GS matrices, however, the correlation of isozyme matrix vs. RAPD and ISSR pattern was low. GS coefficients of isozyme, RAPD and ISSR were used to cluster the genotypes based on the UPGMA method and the dendrograms obtained were compared to develop consensus phylogenetic trees using NTSYSpc. The X-axis contained IISR Comp-1 and R-06, and the Y-axis contained LS-6 and LKC-11. The much lower values of average genetic similarity based on RAPD (0.27) and ISSR (0.35) indicated the ability of DNA based markers to detect high degree of polymorphism among these populations suggesting thereby, the possibility of screening a higher number of anonymous loci in sugarbeet using these molecular markers as compared to isozymes to enable the selection of the best parents in order to obtain new genetic combinations.

show abstract

“…There is a plethora of molecular markers based on the polymerase chain reaction (PCR) and over the last 10 years a large body of information about them has accumulated [15,42]. These markers have been used in wild plants to uncover genetic diversity and relatedness in plant populations, to measure the influence of breeding systems on diversity, and in plant taxonomy [4,9,10,17,20]. With cultivated plants they have been used in cultivar identification, to determine genetic relatedness among cultivars, for paternity testing, in the identification of hybrids and in genome mapping projects [1-3, 19, 21, 25, Burkart [7,8].…”

mentioning

confidence: 99%

Analysis of the relationships between species of the genus Prosopis revealed by the use of molecular markers

et al. 1999

View full text Add to dashboard Cite

show abstract

RAPD variation in wild populations of four species of the genus Hordeum (Poaceae)

Cited by 55 publications

References 31 publications

Search for evidence of introgression of wheat (Triticum aestivum L.) traits into sea barley (Hordeum marinum s.str. Huds.) and bearded wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers

Search for evidence of introgression of wheat (Triticum aestivum L.) traits into sea barley (Hordeum marinum s.str. Huds.) and bearded wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers

Genetic Diversity Analysis in Sugarbeet (Beta vulgaris L.) Using Isozymes, RAPD and ISSR Markers

Analysis of the relationships between species of the genus Prosopis revealed by the use of molecular markers

Contact Info

Product

Resources

About