Rapid 3D bioprinting of a multicellular model recapitulating pterygium microenvironment

Zhong, Zheng; Wang, Jing; Tian, Jing; Deng, Xiaoqian; Balayan, Alis; Sun, Yukun; Xiang, Yang; Guan, Jiaao; Schimelman, Jacob; Hwang, Henry H.; You, Shangting; Wu, Xiaokang; Ma, Chao; Shi, Xiaoao; Yao, Emmie; Deng, Sophie X.; Chen, Shaochen

doi:10.1016/j.biomaterials.2022.121391

Cited by 20 publications

(29 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of note, DLP (bio)printers have been widely designed and applied in drug screening, [60] disease modeling, [33] and tissue regeneration. [61] For example, Gou et al designed and developed a liver-inspired 3D tissue model which allows toxins to be trapped efficiently.…”

Section: Resolution (µM) Applicationmentioning

confidence: 99%

“…[141,142] In order to investigate the mechanism of conjunctival disease, 3D-engineered models obtained by DLP bioprinting strategy have become an effective strategy to create ocular surface disease modeling for high-throughput drug screening. [33,[143][144][145][146][147] To obtain a 3D-engineered models, Zhong et al designed and produced a rabbit-derived conjunctival stem cells (CjSCs) loaded GelMA hydrogel model via DLP-based bioprinting for injectable delivery (Figure 7A). [143] In their work, the stiffness of the hydrogel micro-constructs could be adjusted by changing the light exposure time, which could maintain the viability and stem cell behavior of encapsulated CjSCs for dynamic suspension culture of CjSCs.…”

Section: Dlp Bioprinting For Eye Repairmentioning

confidence: 99%

“…[12][13][14] Currently, 3D bioprinting improved the application of tissue structure in drug screening, [15,16] disease modeling, [17,18] and tissue repair and regenerative medicine. [19,20] Most of importantly, heart, [21,22] blood vessels, [23,24] bone, [25,26] cartilage, [27,28] liver, [1,29,30] lung, [1,31] eye, [32,33] neuronal tissue, [34,35] and pancreatic tissue [36] have been successfully designed and developed through 3D extrusion-based bioprinting and DLPbased bioprinting strategies (Figure 1A). However, the resolution of extrusion-based 3D bioprinting structure is relatively limited, and the resolution of conventional extrusion printing is usually less than 200 μm, and the obtained structure is also easy to cause stress relaxation and permanent deformation due to the different crosslinking rate during the printing process.…”

Section: Introductionmentioning

confidence: 99%

“…Of note, DLP (bio)printers have been widely designed and applied in drug screening, [ 60 ] disease modeling, [ 33 ] and tissue regeneration. [ 61 ] For example, Gou et al.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Digital light processing (DLP)‐based (bio)printing strategies for tissue modeling and regeneration

Dai

Wang

et al. 2022

Aggregate

View full text Add to dashboard Cite

Digital light processing (DLP)-based bioprinting technology has recently aroused considerable concerns as a strategy to deliver biomedical materials and/or specific cells to create sophisticated structures for various tissue modeling and regeneration. In this review, we display a concise introduction of DLP bioprinting, and a further discussion on the design and manufacture of DLP (bio)printer with varied bioinks and their biomedical applications toward drug screening, disease modeling, tissue repair, and regenerative medicine. Finally, the advantages, challenges, and perspectives of the DLP printing platforms are detailed. It is believed that DLP bioprinting will play a decisive role in the field of tissue model and regenerative medicine, mainly due to its time-efficient, higher resolution, and amenability to automation for various tissue needs.

show abstract

Section: Resolution (µM) Applicationmentioning

confidence: 99%

Section: Dlp Bioprinting For Eye Repairmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Of note, DLP (bio)printers have been widely designed and applied in drug screening, [ 60 ] disease modeling, [ 33 ] and tissue regeneration. [ 61 ] For example, Gou et al.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Digital light processing (DLP)‐based (bio)printing strategies for tissue modeling and regeneration

Dai

Wang

et al. 2022

Aggregate

View full text Add to dashboard Cite

show abstract

“…Three-dimensionally (3D) engineered tissues are artificial functional tissues composed of biomaterial scaffolds and living cells (1)(2)(3). Engineered tissues have found many biomedical applications, including basic biomedical research, disease modeling, drug testing, personalized medicine, regenerative medicine, and organ transplantation (4)(5)(6)(7)(8). 3D-engineered tissues are expected to accurately recapitulate the 3D architecture, cell types, and physical and biochemical environment of the native tissues, providing in vitro tissue or organ models with better biorelevance, scalability, and reproducibility compared to traditional 2D monolayer cell models or animal models (9).…”

Section: Introductionmentioning

confidence: 99%

High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues

et al. 2023

Self Cite

View full text Add to dashboard Cite

Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing–based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture.

show abstract

ECM‐Like Adhesive Hydrogel for the Regeneration of Large Corneal Stromal Defects

Wang

Zhang

Qiao

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

The repair of large‐diameter corneal stroma defects is a major clinical problem. Although some studies have attempted to use hydrogels to repair corneal damage, most of these hydrogels can only be used for focal stromal defects that are ≤3.5 mm in diameter due to poor hydrogel adhesion. Here, a photocurable adhesive hydrogel that mimics the extracellular matrix (ECM) with regard to composition for repairing 6 mm‐diameter corneal stromal defects in rabbits is investigated. This ECM‐like adhesive can be rapidly cured after light exposure, with high light transmittance and good mechanical properties. More importantly, this hydrogel maintains the viability and adhesion of cornea‐derived cells and promotes their migration in vitro in 2D and 3D culture environments. Proteomics analysis confirms that the hydrogel promotes cell proliferation and ECM synthesis. Furthermore, in rabbit corneal stromal defect repair experiments, it is proven by histological and proteomic analysis that this hydrogel can effectively promote corneal stroma repair, reduce scar formation, and increase corneal stromal–neural regeneration at the six months follow‐up. This work demonstrates the great application of ECM‐like adhesive hydrogels for the regeneration of large‐diameter corneal defects.

show abstract

Rapid 3D bioprinting of a multicellular model recapitulating pterygium microenvironment

Cited by 20 publications

References 80 publications

Digital light processing (DLP)‐based (bio)printing strategies for tissue modeling and regeneration

Digital light processing (DLP)‐based (bio)printing strategies for tissue modeling and regeneration

High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues

ECM‐Like Adhesive Hydrogel for the Regeneration of Large Corneal Stromal Defects

Contact Info

Product

Resources

About