Rapid activation of specific phospholipase(s) D by cytokinin in<i>Amaranthus</i>assay system

Kravets, V. S.; Kolesnikov, Yaroslav S.; Kretynin, Sergey V.; Getman, Irina A.; Romanov, Georgy A.

doi:10.1111/j.1399-3054.2009.01324.x

Cited by 13 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, there are data on the involvement of PLD in the action of gibberellins [28] and in auxin transport [184,185] (table). PLD also plays a role in the activation by cytokinins of the primary response genes [77,186,187], and a rapid increase in PLD activity is observed under the influence of cytokinins [69,76]. The PLDs involved in transmission of signals of different phytohormones are also different.…”

Section: +mentioning

confidence: 99%

“…The PLDs involved in transmission of signals of different phytohormones are also different. The abscisic acid signal is transmitted with involvement of phospholipase D isoforms α and partially β (table) [28,181], whereas the cytokinin signal is transmitted not with involvement of the PLD α-isoform, but more likely with the involvement of PLDγ [76]. Thus, PLD is an important and specific factor in the signal transmission of various hormones in both animals and plants.…”

Section: +mentioning

confidence: 99%

“…Calcium plays an important role as a modifier of PLD activity in plant cells, in particular under the influence of cytokinin [70] and also as a cofactor of PLD isoenzymes containing the C2 domain [2,43,61]. Calcium ions activate PLDα and PLDβ through binding with the C2 domain.…”

Section: Regulatory Mechanisms Of Pld Activitymentioning

confidence: 99%

“…And PLD is often contributes to signal transmission through production of phosphatidic acid as a secondary messenger. In particular, PLD is involved in transmission of signals of classic plant hormones such as abscisic acid [11] and cytokinin [69].…”

Section: Regulatory Mechanisms Of Pld Activitymentioning

confidence: 99%

“…PLDs are regulated by various lipids, and among them polyphosphoinositides are considered to be especially important modifiers of cytoskeleton dynamics, membrane transport, and enzyme activity [75]. PLDs sensitive to these phospholipids are involved in transmission of intracellular signals of hormones, in particular of cytokinins in plants [76][77][78]. In animal cells the catalytic activities of PLD1 and PLD2 are sensitive to PtdIns(4,5)P 2 , which acts as their cofactor [26].…”

Section: +mentioning

confidence: 99%

See 4 more Smart Citations

Molecular structure of phospholipase D and regulatory mechanisms of its activity in plant and animal cells

Kolesnikov

Nokhrina

Kretynin

et al. 2012

Biochemistry Moscow

View full text Add to dashboard Cite

Abbreviations: Gα, α-subunit of heterotrimeric G-protein; PKC, protein kinase C; PLD, phospholipase D; PtdIns(4,5)P 2 , phosphatidylinositol-4,5-bisphosphate. * To whom correspondence should be addressed.Abstract⎯Phospholipase D (PLD) catalyzes hydrolysis of phospholipids with production of phosphatidic acids, which often act as secondary messengers on transmission of intracellular signals. This review summarizes data of various leading laboratories on specific features of organization and regulation of PLD activity in plant and animal cells. The main structural domains of PLD (C2, PX, PH), the active site, and other functionally important parts of the enzyme are considered. Regulatory mechanisms of PLD activity are characterized in detail. Studies associated with molecular design, analysis, and synthesis of new nontoxic substances capable of inhibiting different PLD isoenzymes in vivo are shown to be promising for biotechnology and medicine.

show abstract

Section: +mentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Section: Regulatory Mechanisms Of Pld Activitymentioning

confidence: 99%

Section: Regulatory Mechanisms Of Pld Activitymentioning

confidence: 99%

Section: +mentioning

confidence: 99%

See 3 more Smart Citations

Molecular structure of phospholipase D and regulatory mechanisms of its activity in plant and animal cells

Kolesnikov

Nokhrina

Kretynin

et al. 2012

Biochemistry Moscow

View full text Add to dashboard Cite

show abstract

Phosphoglycerolipids are master players in plant hormone signal transduction

et al. 2013

View full text Add to dashboard Cite

Phosphoglycerolipids are essential structural constituents of membranes and some also have important cell signalling roles. In this review, we focus on phosphoglycerolipids that are mediators in hormone signal transduction in plants. We first describe the structures of the main signalling phosphoglycerolipids and the metabolic pathways that generate them, namely the phospholipase and lipid kinase pathways. In silico analysis of Arabidopsis transcriptome data provides evidence that the genes encoding the enzymes of these pathways are transcriptionally regulated in responses to hormones, suggesting some link with hormone signal transduction. The involvement of phosphoglycerolipid signalling in the early responses to abscisic acid, salicylic acid and auxins is then detailed. One of the most important signalling lipids in plants is phosphatidic acid. It can activate or inactivate protein kinases and/or protein phosphatases involved in hormone signalling. It can also activate NADPH oxidase leading to the production of reactive oxygen species. We will interrogate the mechanisms that allow the activation/deactivation of the lipid pathways, in particular the roles of G proteins and calcium. Mediating lipids thus appear as master players of cell signalling, modulating, if not controlling, major transducing steps of hormone signals.

show abstract

Chemical proteomic analysis of 6-benzylaminopurine molecular partners in wheat grains

et al. 2017

View full text Add to dashboard Cite

An affinity-based chemical proteomic technique enabled direct identification of BAP-interacting proteins in wheat, including the well-known cytokinin-binder, cytokinin-binding protein 1. In this work, we show the development of a chemical proteomic technique for the identification of proteins binding to natural aromatic cytokinins (CKs). 6-benzylaminopurine (BAP) and documented CK-binder, wheat germ-allocated cytokinin-binding protein 1 (CBP-1), were suggested as an ideal proof-of concept affinity pair. Therefore, wheat grains were chosen as a model plant material. The BAP affinity beads were prepared by the immobilization of synthesized BAP-derived ligand to a commercial, pre-activated resin and used to isolate target proteins. The proteomic analysis of complex plant extracts is often complicated by the presence of highly abundant background proteins; in this case, the omnipresent alpha-amylase inhibitors (AAIs). To cope with this problem, we included SDS-PAGE, in-gel trypsin digestion and fraction pooling prior to shotgun analysis, which brought about an obvious drop in the signals belonging to the obstructing proteins. This was accompanied by a sharp increase in the number of identified BAP targets in comparison to a conventional in-solution digestion approach. To distinguish specific CK-binding proteins from those having a general affinity for nucleotide-like compounds, competitive pull-downs with natural nucleotides and free BAP were included in every affinity experiment. By this approach, we were able to identify a group of BAP-interacting proteins, which were subsequently found to be related to biological processes affected by CKs. Moreover, the selected affinity enrichment strategy was verified by the detection of the aforementioned CK-interacting protein, CBP-1. We propose that the developed method represents a promising tool for appealing research of as yet unknown CK molecular partners in plants.

show abstract

Rapid activation of specific phospholipase(s) D by cytokinin inAmaranthusassay system

Cited by 13 publications

References 38 publications

Molecular structure of phospholipase D and regulatory mechanisms of its activity in plant and animal cells

Molecular structure of phospholipase D and regulatory mechanisms of its activity in plant and animal cells

Phosphoglycerolipids are master players in plant hormone signal transduction

Chemical proteomic analysis of 6-benzylaminopurine molecular partners in wheat grains

Contact Info

Product

Resources

About