Rapid analysis of forensic‐related samples using two ambient ionization techniques coupled to high‐resolution mass spectrometers

Jagerdeo, Eshwar; Wriston, Amanda

doi:10.1002/rcm.7844

Cited by 16 publications

(14 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using an extraction time of 5 min, a desorption time of 12 s, and a spray time of 1.8 s with the dwell time set at 5 ms, we were able to achieve figures of merit for the suite of model compounds that were comparable to those obtained in short-spray experiments (Table ). Moreover, we were able to achieve a total sample analysis time of less than 10 s (3.2 s [sample preparation time] + 1.8 s [instrument time]); as such, the automated CBS’ analysis speed for detecting drugs of abuse in urine is comparable to online-SPE-MS and LDTD methods, both of which are recognized for their speed. , However, the signal generated via CBS is not Gaussian; it is an adjustable continuous square wave. Thus, the number of compounds that can be monitored is not limited by mass spectrometer performance (i.e., scan rate) but by signal generation time.…”

Section: Results and Discussionmentioning

confidence: 99%

Breaching the 10 Second Barrier of Total Analysis Time for Complex Matrices via Automated Coated Blade Spray

Kasperkiewicz

Gómez‐Ríos

Hein³

et al. 2019

Anal. Chem.

View full text Add to dashboard Cite

In the development of modern analytical workflows, parameters such as sample turnaround time, cost of analysis, and ease of use must be prioritized. Automation enables reductions in total analysis time, human intervention, and cost per sample. In this report, a suitable automated coated blade spray (CBS) workflow is proposed for the screening and quantitation of multiple substances (i.e., drugs of abuse and pesticides) in complex matrices. In an attempt to reduce the total sample analysis time, several parameters were investigated, including tandem mass spectrometry (MS) dwell time, CBS spray time, and extraction time. Solid-phase microextraction (SPME) method parameters are explored, such as reduction of extraction time for increased signal-to-noise. Model compounds with a moderately wide range of molecular weights (150–500 Da), polarities, and structural diversity were selected in order to monitor analytical figures of merit during method optimization. The resultant automated CBS method proved capable of analyzing the model compounds in human urine in under 10 s total analysis time with excellent accuracy (95–120%) and precision (RSD < 12%). As an application, an automated method for the screening and quantitation of more than 150 pesticides from apple juice was demonstrated on both triple quadrupole and orbitrap instruments in under 15 s total sample analysis time.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Breaching the 10 Second Barrier of Total Analysis Time for Complex Matrices via Automated Coated Blade Spray

Kasperkiewicz

Gómez‐Ríos

Hein³

et al. 2019

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…. 47 The presence of the targeted compounds was established using the accurate masses of the protonated molecule [M + H] + and one of its major isotopes, both with mass tolerance of 5 mmu. For confirmation of identity, a targeted MS 2 experiment was performed.…”

Section: Resultsmentioning

confidence: 99%

Rapid screening procedures for a variety of complex forensic samples using laser diode thermal desorption (LDTD) coupled to different mass spectrometers

Jagerdeo

Auger

2022

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

The applications shared in this paper demonstrate the wide variety of samples that can be analyzed when Laser Diode Thermal Desorption (LDTD) is interfaced with a high-resolution mass spectrometer and show the speed at which high quality data can be generated from complex matrices.Methods: Samples are solvent extracted and spotted in a 96-well plate. In the case of biological fluids, hydrolysis followed by solid-phase extraction is required. The solvent in the 96-well plate is evaporated followed by mass spectrometric (MS) analysis with atmospheric pressure chemical ionization. Where applicable, the instrument is operated in data-dependent mode, with a full-scan mass spectrum followed by MS/MS spectra of the top 10 ions with a total runtime of 0.4 min.Results: Four applications (MAAQ and Tear Gas, twelve rodenticides, seven

show abstract

“…Details in ref. [11] techniques coupled to HRMS [45], namely by laser diode thermal desorption or atmospheric solids analysis probe allowing fast analysis of a wide range of samples with minimal or no sample preparation. This ambient coupling confirmed again the universality of HRMS.…”

Section: Screening For Detection Of Drugs Poisons And/or Their Metamentioning

confidence: 99%

Hyphenated high-resolution mass spectrometry—the “all-in-one” device in analytical toxicology?

Maurer

2020

Anal Bioanal Chem

View full text Add to dashboard Cite

This trend article reviews papers with hyphenated high-resolution mass spectrometry (HRMS) approaches applied in analytical toxicology, particularly in clinical and forensic toxicology published since 2016 and referenced in PubMed. The article focuses on the question of whether HRMS has or will become the all-in-one device in these fields as supposed by the increasing number of HRMS presentations at scientific meetings, corresponding original papers, and review articles. Typical examples for the different application fields are discussed such as targeted or untargeted drug screening, quantification, drug metabolism studies, and metabolomics approaches. Considering the reviewed papers, HRMS is currently the only technique that fulfills the criteria of an all-in-one device for the various applications needed in analytical toxicology.Graphical abstract

show abstract

Rapid analysis of forensic‐related samples using two ambient ionization techniques coupled to high‐resolution mass spectrometers

Cited by 16 publications

References 43 publications

Breaching the 10 Second Barrier of Total Analysis Time for Complex Matrices via Automated Coated Blade Spray

Breaching the 10 Second Barrier of Total Analysis Time for Complex Matrices via Automated Coated Blade Spray

Rapid screening procedures for a variety of complex forensic samples using laser diode thermal desorption (LDTD) coupled to different mass spectrometers

Hyphenated high-resolution mass spectrometry—the “all-in-one” device in analytical toxicology?

Contact Info

Product

Resources

About