Rapid analysis of isotopically unmodified amino acids by high-resolution 14N-edited 1H–13C correlation NMR spectroscopy

Abstract

A (14)N-edited (1)H-(13)C NMR method is described for structural analysis under high-resolution of biomolecules without any enrichment.

Measurement of hetero-nuclear distances using a symmetry-based pulse sequence in solid-state NMR

“…The S-RESPDOR 13 C-14 N distance determination can be combined with the analysis of the 1 H-13 C ( 14 N-edited) HETCOR spectrum, which only shows carbons that are close to 14 N atoms. 50…”

14N and 81Br Quadrupolar Nuclei as Sensitive NMR Probes of n-Alkyltrimethylammonium Bromide Crystal Structures. An Experimental and Theoretical Study

“…In parallel, different indirect methods (spin-1 / 2 detection) have been developed to estimate 14 N NMR interactions, [28][29][30][31] and very recently to record 14 N spectra. [32][33][34] Overtone 14 N NMR spectroscopy, [35][36][37] aiming at acquiring directly double quantum spectra, has been investigated by several groups over the last years but has not succeeded yet in reaching the required efficiency for their use in real material science applications. For tetraalkylammonium salts, only a few recent studies have been devoted to their detailed solid state 14 N MAS NMR characterization 38,39 which complete earlier data obtained under static conditions.…”

“…The characterization of solid samples by 14 N MAS NMR has been attracting more attention over the last 10 years, − facing the problem of large quadrupolar coupling constants C Q encountered in the case of amino acid functionality. In parallel, different indirect methods (spin- 1 / 2 detection) have been developed to estimate 14 N NMR interactions, − and very recently to record 14 N spectra. − Overtone 14 N NMR spectroscopy, − aiming at acquiring directly double quantum spectra, has been investigated by several groups over the last years but has not succeeded yet in reaching the required efficiency for their use in real material science applications. For tetraalkylammonium salts, only a few recent studies have been devoted to their detailed solid state 14 N MAS NMR characterization , which complete earlier data obtained under static conditions. , In the case of n -alkyltrimethylammonium bromide salts C x H 2 x +1 (CH 3 ) 3 N + Br − with long chains ( x > 4), 14 N static NMR studies have been published earlier for the hexadecyltrimethylammonium bromide (HTAB, x = 16) and decyltrimethylammonium bromide ( x = 10) .…”

Very fast magic angle spinning 1H-14N 2D solid-state NMR: Sub-micro-liter sample data collection in a few minutes

“…Detecting dynamics on 100 ns time scale [18] has also been achieved using the molecular motion sensitive SQ coherences in the 1 H-14 N HMQC experiment. 1 H-14 N D-HMQC filtering methods for spectral editing [19,20], and three dimensional correlation among 1 H, 13 C, and 14 N [11] have also been reported.…”