Rapid analysis of neomycin in cochlear perilymph of guinea pigs using disposable SPE cartridges and high performance liquid chromatography-tandem mass spectrometry

Zu, Mian; Jiang, Jinan; Zhao, Hui; Zhang, Shili; Yan, Yan; Qiu, Shuo; Yuan, Shuolong; Han, Jia-Wei; Zhang, Yue; Guo, Weiwei; Yang, Shiming

doi:10.1016/j.jchromb.2018.06.055

Cited by 13 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice were anesthetized using an intraperitoneal injection of pentobarbital sodium (50 mg/kg) and kept on a thermal insulation blanket. Click stimuli or pure tone stimuli from 2 to 16 kHz were generated by Tucker Davis Technologies System (TDT) and delivered by a MF1 speaker (TDT) [ 27 ]. The intensity of the tone stimuli was calibrated using a sound level meter with 1/4-inch pressure-field microphone (B&K).…”

Section: Methodsmentioning

confidence: 99%

SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

Guo

Cong

et al. 2021

BMC Neurosci

Self Cite

View full text Add to dashboard Cite

Background The SCN11A gene, encoded Nav1.9 TTX resistant sodium channels, is a main effector in peripheral inflammation related pain in nociceptive neurons. The role of SCN11A gene in the auditory system has not been well characterized. We therefore examined the expression of SCN11A in the murine cochlea, the morphological and physiological features of Nav1.9 knockout (KO) ICR mice. Results Nav1.9 expression was found in the primary afferent endings beneath the inner hair cells (IHCs). The relative quantitative expression of Nav1.9 mRNA in modiolus of wild-type (WT) mice remains unchanged from P0 to P60. The number of presynaptic CtBP2 puncta in Nav1.9 KO mice was significantly lower than WT. In addition, the number of SGNs in Nav1.9 KO mice was also less than WT in the basal turn, but not in the apical and middle turns. There was no lesion in the somas and stereocilia of hair cells in Nav1.9 KO mice. Furthermore, Nav1.9 KO mice showed higher and progressive elevated ABR threshold at 16 kHz, and a significant increase in CAP thresholds. Conclusions These data suggest a role of Nav1.9 in regulating the function of ribbon synapses and the auditory nerves. The impairment induced by Nav1.9 gene deletion mimics the characters of cochlear synaptopathy.

show abstract

Section: Methodsmentioning

confidence: 99%

SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

Guo

Cong

et al. 2021

BMC Neurosci

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, mass spectrometry can analyze small sample volumes with high precision, sensitivity, and selectivity [68,81]. In this review, 15 articles reported the use of mass spectrometry as a detector for the bioanalysis of neomycin, streptomycin, dihydrostreptomycin, amikacin, kanamycin, paromomycin, tobramycin, spectinomycin, apramycin, hygromycin, gentamicin, and arbekacin [5,31,[33][34][35]68,69,72,73,75,79,81,[83][84][85]. Interestingly, articles published between 1977 [20] to 2002 [67] were dominated by fluorescence or UV detection methods.…”

Section: A D V a N C E D O N L I N E A R T I C L Ementioning

confidence: 99%

“…Method performance characteristics reported by most articles included calibration range, linearity, recovery, repeatability, and intermediate precision (Table 2). Out of the 71 articles reviewed, only four reported on matrix effect [63,[83][84][85]. Resolution was reported by one article [75].…”

Section: Performance Metricsmentioning

confidence: 99%

Bioanalysis of aminoglycosides using high-performance liquid chromatography

et al. 2022

View full text Add to dashboard Cite

Aminoglycosides are broad-spectrum antibiotics used in the treatment of gram-negative bacterial infections. Due to their nephrotoxic and ototoxic potential (narrow therapeutic index), the use of aminoglycoside for clinical indications requires monitoring. The objective of this review was to identify relevant literature reporting liquid chromatographic methods for the bioanalysis of aminoglycosides in both preclinical and clinical settings/experiments. Data on liquid chromatographic methods were collected from articles in an online academic database (PubMed, Science Direct, Scopus, and Google Scholar). All 71 articles published from 1977 to 2020 were included in the review. Reversed-phase liquid chromatography was the most used method for the bioanalysis of aminoglycosides. Fluorescence or ultraviolet detection methods were mostly used from 1977 to 2002 (51 articles), while mass spectrometry was predominantly used as a detector from 2003 to 2020 (15 articles). Sixty-seven articles reported calibration ranges, which varied significantly for the various drugs assayed: some in the range of 0.1-0.5 ng/mL and others 1250-200000 ng/mL. Also, 61 articles reported R2 values (0.964-1.0) for almost all analytes under consideration. Sixty-three articles reported percent recoveries mostly between 61.0 % to 114.0 %, with only two articles reporting recoveries of 4.9 % and 36 %. Out of the 71 reviewed articles, 56 reported intermediate precision values ranging between 0.331 % to 19.76 %, which is within the acceptable limit of 20 %. This review will serve as a guide for research and/or routine clinical monitoring of aminoglycosides in biological matrices.

show abstract

“…Mice were anesthetized using an intraperitoneal injection of pentobarbital sodium (50 mg/Kg) and kept on a thermal insulation blanket. Click stimuli or pure tone stimuli from 2 to 16 kHz were generated by Tucker Davis Technologies System (TDT) and delivered by a MF1 speaker (TDT) [26]. The intensity of the tone stimuli was calibrated using a sound level meter with 1/4-inch pressure-eld microphone (B&K).…”

Section: Auditory Brainstem Response (Abr) and Ecochg Recordingmentioning

confidence: 99%

SCN11A Gene Deletion Causes Sensorineural Hearing Loss by Impairing the Ribbon Synapses and Auditory Nerves

Guo

Cong

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The SCN11A gene, encoded Nav1.9 TTX resistant sodium channels, is a main effector in peripheral inflammation related pain in nociceptive neurons. The role of SCN11A gene in the auditory system has not been well characterized. We therefore examined the expression of SCN11A in the murine cochlea, the morphological and physiological features of Nav1.9 knockout (KO) ICR mice. Results: Nav1.9 expression was found in the primary afferent endings beneath the inner hair cells (IHCs). The relative quantitative expression of Nav1.9 mRNA in modiolus of wild-type (WT) mice remains unchanged from P0 to P60. The number of presynaptic CtBP2 puncta in Nav1.9 KO mice was significantly lower than WT. In addition, the number of SGNs in Nav1.9 KO mice in the basal turn was also lower than WT, but not in the apical and middle turns. There was no lesion in the somas and stereocilia of hair cells in Nav1.9 KO mice. Nav1.9 KO mice showed higher and progressive ABR threshold at 16 kHz, a significant increase in CAP thresholds, while no changes in cochlear microphonics (CM). Conclusions: These data suggest a role of Nav1.9 in regulating the function of ribbon synapses and the auditory nerves. The impairment induced by Nav1.9 gene deletion mimics the characters of cochlear synaptopathy.

show abstract

Rapid analysis of neomycin in cochlear perilymph of guinea pigs using disposable SPE cartridges and high performance liquid chromatography-tandem mass spectrometry

Cited by 13 publications

References 33 publications

SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

Bioanalysis of aminoglycosides using high-performance liquid chromatography

SCN11A Gene Deletion Causes Sensorineural Hearing Loss by Impairing the Ribbon Synapses and Auditory Nerves

Contact Info

Product

Resources

About