Rapid analysis of porphyrins at low ng/l and μg/l levels in human urine by a gradient liquid chromatography method using octadecylsilica monolithic columns

A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine

“…The aim of this work is to develop a novel method for the simultaneous quantitative determination of urinary porphyrins. So far several methods have been proposed for the determination and screening of urinary porphyrins [4][5][6][7][8][9][10]. However, most of them, either did not quantify CP and UP separately or the quantitative determination of the individual components of urinary porphyrins was achieved using expensive, tedious and time-consuming separation processes.…”

Validação de metodologia para dosagem de porfirinas urinárias por cromatografia líquida de alta eficiência

“…Zuijderhoudt et al (2000), em estudos de precisão da dosagem de porfirinas por CLAE, encontraram CV menor que 15% para URO, HEPTA e COPRO. Bozek, Hutta, Hrivnáková (2005) obtiveram CV de 1,9 a 3,1% para precisão interensaio. Ambos estão de acordo com os resultados obtidos neste trabalho.…”

Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

“…Porphyrins are measured to diagnose and manage porphyrias. Separation and quantification of isomers I and III for uroporphyrins and coproporphyrins is achieved in 4.0 min by HPLC-fluorimetric detection with an LOQ of 82 pmol/L for uroporphyrin I, 44 pmol/L for uroporphyrin III, 50 pmol/L for coproporphyrin I, and 47 pmol/L for coproporphyrin III, and a linear range of 8-180 nmol/L for all [47]. Another HPLC-fluorimetric detection method was reported to measure porphyrins in urine, feces, or plasma [48].…”