Rapid analysis of the effect of aminoglycosides on bacteria by using a sensor system based on a piezoelectric resonator with a lateral electric field

Abstract

The possibility of express analysis of the effect of aminoglycoside antibiotics on bacteria (Escherichia coli) using a sensor system based on a piezoelectric resonator with a lateral electric field with an operating frequency range of 6–7 MHz is shown. E.coli strains, both sensitive and resistant to kanamycin were used for the experiments. During evaluating the kanamycin effect on bacteria, the change in the electrical impedance modulus of the resonator was used as an analitical signal. It has been established that the criterion for the antibiotic bacteria sensitivity is the change in the modulus of the sensor electrical impedance after antibiotic exposure on bacteria at any frequency near the resonance. The sensor is highly sensitive and allows diagnosing the antimicrobial susceptibility of bacteria within 7–9 minutes.

Electroacoustic Biosensor Systems for Evaluating Antibiotic Action on Microbial Cells

“…The prospects for using a biological sensor based on a piezoelectric resonator with a lateral electric field to evaluate the effect of antibiotics (ampicillin and kanamycin) on E. coli cells and analyze their antibiotic sensitivity are shown in [ 89 , 90 ]. The general scheme of the described sensor is shown in Figure 8 .…”

“…The obtained results demonstrate the promise of a piezoelectric resonator with a lateral electric field for the analysis of antibiotic sensitivity/resistance of bacteria. By using the sensor described above, a comparative analysis was done of the sensitivity of bacteria to β-lactams [ 84 ] and aminoglycosides [ 92 ].…”