Rapid analysis of triacylglycerols using high-performance liquid chromatography with light scattering detection

Palmer, Antony J.; Palmer, Fiona J.

doi:10.1016/s0021-9673(01)92674-3

Cited by 58 publications

(24 citation statements)

References 17 publications

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“…This is in agreement with the fatty acid data (Table 5) and with previous work. [2][3][4][5]26,27 In both cases oleic acid takes priority over linoleic at the 2-position. In total, 5 DAGs and 19 TAGs were identified in hazelnut oil and 5 DAG and 29 TAG species in olive oil.…”

Section: Survey Of Vegetable Oilsmentioning

confidence: 99%

“…Separation is based on both the combined chain length of the fatty acyl residues and on the total number of double bonds in the molecule. A range of detection methods, including evaporative light scattering, 2,3 refractive index [4][5][6] and flame ionization detection 7 have been employed in the analysis of TAGs. However, identification of components using these methods relies on relative retention times or collection of peaks followed by mass spectrometric analysis.…”

mentioning

confidence: 99%

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Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Mottram

Woodbury

Evershed

1997

Rapid Commun. Mass Spectrom.

186

123

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Non-aqueous reversed-phase high performance liquid chromatography (HPLC) has become an established means of separating the triacylglycerols (TAGs) present in natural fats and oils.1 The most efficient separations have been achieved with columns packed with chemically bonded octadecylsilyl phases, employing mobile phases consisting of acetonitrile with acetone, propionitrile or chlorinated solvents. Separation is based on both the combined chain length of the fatty acyl residues and on the total number of double bonds in the molecule. A range of detection methods, including evaporative light scattering, 2,3 refractive index [4][5][6] and flame ionization detection 7 have been employed in the analysis of TAGs. However, identification of components using these methods relies on relative retention times or collection of peaks followed by mass spectrometric analysis. With very complex mixtures such as milk fat or fish oils, identification of TAGs in this way becomes impractical. Consequently mass spectrometric detection, which provides detailed information on the molecular weight and fatty acid composition of TAG molecular species, as well as allowing identification of partially resolved or co-eluting peaks, is becoming increasingly widespread.Whilst + , which allow identification of the fatty acids present in each TAG species. 9 The technique has been applied to the analysis of several vegetable oils. [10][11][12] In addition to determining which fatty acids are present in a TAG, it is also important to be able to determine their positions on the glycerol backbone of the molecule since this has considerable biochemical and nutritional significance.13,14 The ability to unambiguously identify positional isomers of individual components of TAG mixtures, is a long standing problem in lipid chemistry. One method for stereospecific analysis of TAGs involves using enzymatic hydrolysis to sequentially cleave the fatty acids at sn-1 and sn-2. These are then analysed directly and the most probable fatty acid at sn-3 calculated. 15 Christie et al. 16 have developed a method in which TAGs are partially hydrolysed and derivatized to diacylglycerol urethanes which can be analysed by gas chromatography. Both of these methods, however, are time consuming. In enzymatic hydrolysis there may be some selectivity for certain chain lengths and numbers of double bonds, as well as a possibility of acyl migration during the analysis. Similarly, urethane derivatization is not suitable for the analysis of more complex fats and oils. Argentation HPLC, using a silver-ion impregnated column in conjunction with polar solvents, has been demonstrated to separate ABA and AAB type positional isomers, where A and B represent different fatty acyl moieties, 17 but gives no information on the positions of fatty acids in an ABC type TAG. A recent paper described the analysis of a number of γ-linolenic-acid-containing vegetable oils using silver-ion HPLC in conjunction with APCI mass spectrometry. 18 We recently showed that positional isomers of TAGs can be...

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Section: Survey Of Vegetable Oilsmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Mottram

Woodbury

Evershed

1997

Rapid Commun. Mass Spectrom.

186

123

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“…In recent years, soya bean oil has often been used to triacylglycerol (TAG) mixtures: (i) the large number of illustrate the high-resolution power of RP-HPLC species with dienoic and trienoic fatty acid (FA) moi- (Goiffon et a1 1981a;Frede 1986; Barron and Santaeties to be separated and (ii) the wide range of proporMaria 1989; Palmer and Palmer 1989) for direct separations of the TAG. Because of this, the chromatographic tion of molecular species.…”

Section: Introductionmentioning

confidence: 99%

Determination of Triacylglycerol Classes and Molecular Species in Seed Oils with High Content of Linoleic and Linolenic Fatty Acids

Tarandjiiska

Marekov

Nikolova‐Damyanova

et al. 1996

J. Sci. Food Agric.

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Soya bean and linseed oils were analysed by successive application of silver‐ion and reversed‐phase thin‐layer chromatography and densitometry. Direct quantitative determination of all the 20 possible triacylglycerol (TAG) classes including critical pairs (SD2+S2T; M2T+D3; SDT+MDT; ST2+MT2) was performed. TAG molecular species down to 0·1% (32 in soya bean oil and 26 in linseed oil) were identified and quantified. The detection limit was 0·2 μg and the standard deviation between 0·1% and 1·0% for silver ion thin‐layer chromatography (TLC) and between 0.1% and 3.9% for reversed‐phase TLC determinations. The accuracy of the analysis was confirmed by the good agreement between the quantitative results for the fatty acid composition as determined by gas chromatography and as calculated from the densitometric data for the TAG composition.

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“…(Frega et al 1990). The separation efficiency attained with this thermostable polar column is, in fact, comparable to that obtained by HPLC (Dong and Dicesare 1983;Palmer and Palmer 1989). This type of analysis can be particularly useful for quality control of edible oils, butter fat, dairy spreads, cocoa butter, cocoa butter equivalents, coffee, animal fat (chicken, egg-yolk), tobacco seeds (Frega et al 1991).…”

Section: Analysis Of Triacylglycerols (Triglycerides)mentioning

confidence: 57%

Applications on thermostable polar capillary GC columns

Rodríguez‐Estrada¹,

Frega²,

Lercker³

2002

Grasas y Aceites

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Rapid analysis of triacylglycerols using high-performance liquid chromatography with light scattering detection

Cited by 58 publications

References 17 publications

Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Determination of Triacylglycerol Classes and Molecular Species in Seed Oils with High Content of Linoleic and Linolenic Fatty Acids

Applications on thermostable polar capillary GC columns

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