Rapid and Detergent-Free Decellularization of Cartilage

Shen, Wei; Berning, Karsten; Tang, Sze Wing; Lam, Yun Wah

doi:10.1089/ten.tec.2020.0008

Cited by 24 publications

(13 citation statements)

References 23 publications

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“…Insufficient decellularization easily leads to excessive residual immunogenic components. While intense decellularization will result in damage to the ECM ultrastructure and the loss of composition ( Gilpin and Yang, 2017 ; Shen et al, 2020 ). Both run counter to the purpose of decellularization.…”

Section: Immunological Characterization Of Biomaterialsmentioning

confidence: 99%

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Liu

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Biomaterials play a core role in cartilage repair and regeneration. The success or failure of an implanted biomaterial is largely dependent on host response following implantation. Host response has been considered to be influenced by numerous factors, such as immune components of materials, cytokines and inflammatory agents induced by implants. Both synthetic and native materials involve immune components, which are also termed as immunogenicity. Generally, the innate and adaptive immune system will be activated and various cytokines and inflammatory agents will be consequently released after biomaterials implantation, and further triggers host response to biomaterials. This will guide the constructive remolding process of damaged tissue. Therefore, biomaterial immunogenicity should be given more attention. Further understanding the specific biological mechanisms of host response to biomaterials and the effects of the host-biomaterial interaction may be beneficial to promote cartilage repair and regeneration. In this review, we summarized the characteristics of the host response to implants and the immunomodulatory properties of varied biomaterial. We hope this review will provide scientists with inspiration in cartilage regeneration by controlling immune components of biomaterials and modulating the immune system.

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Section: Immunological Characterization Of Biomaterialsmentioning

confidence: 99%

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Liu

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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“…Therefore, monitoring the temperature during sonication and cooling in a chiller are necessary to avoid heating and denaturation of the proteins and molecules. Sonication is mainly used with detergents to decellularize dense tissues, such as tendons, ligaments, and cartilage, as well as thin tissues, such as skin and blood vessels, where sonication allows the detergents to penetrate the tissues more effectively ( Yusof et al, 2019 ; Shen et al, 2020 ; Dang et al, 2021 ; Lin et al, 2021 ; Suss et al, 2021 ). Sonication has only been used to decellularize whole parenchymal organs in the kidney, and has likely not been used in the liver because of the colossal size of liver and the need for high power to disrupt the hepatic microstructure ( Say et al, 2019 ; Manalastas et al, 2021 ).…”

Section: Decellularizationmentioning

confidence: 99%

Recent Advances in Liver Engineering With Decellularized Scaffold

Dai

Jiang

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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Liver transplantation is currently the only effective treatment for patients with end-stage liver disease; however, donor liver scarcity is a notable concern. As a result, extensive endeavors have been made to diversify the source of donor livers. For example, the use of a decellularized scaffold in liver engineering has gained considerable attention in recent years. The decellularized scaffold preserves the original orchestral structure and bioactive chemicals of the liver, and has the potential to create a de novo liver that is fit for transplantation after recellularization. The structure of the liver and hepatic extracellular matrix, decellularization, recellularization, and recent developments are discussed in this review. Additionally, the criteria for assessment and major obstacles in using a decellularized scaffold are covered in detail.

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“…Recently, Shen et al reported a detergent-free decellularization protocol for cartilage, an avascularized tissue, which involves the sonication of presectioned, frozen cartilage in water. 68 The resulting preparations not only retain glycosaminoglycans that are destroyed by other decellularization methods but are potentially compatible with cell sheet technology in tissue engineering. [69][70][71][72] It may be possible to utilize recellularized sections as the building blocks for cell sheets, which are stacked into reconstructed tissues, thus solving the problem of inefficient cell repopulation.…”

Section: Decellularized Tissues As Isolated Cell Nichesmentioning

confidence: 99%

Deconstructing, Replicating, and Engineering Tissue Microenvironment for Stem Cell Differentiation

Tang

Tong

Pang

et al. 2020

Tissue Engineering Part B: Reviews

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One of the most crucial components of regenerative medicine is the controlled differentiation of embryonic or adult stem cells into the desired cell lineage. Although most of the reported protocols of stem cell differentiation involve the use of soluble growth factors, it is increasingly evident that stem cells also undergo differentiation when cultured in the appropriate microenvironment. When cultured in decellularized tissues, for instance, stem cells can recapitulate the morphogenesis and functional specialization of differentiated cell types with speed and efficiency that often surpass the traditional growth factor-driven protocols. This suggests that the tissue microenvironment (TME) provides stem cells with a holistic ''instructive niche'' that harbors signals for cellular reprogramming. The translation of this into medical applications requires the decoding of these signals, but this has been hampered by the complexity of TME. This problem is often addressed by a reductionist approach, in which cells are exposed to substrates decorated with simple, empirically designed geometries, textures, and chemical compositions (''bottom-up'' approach). Although these studies are invaluable in revealing the basic principles of mechanotransduction mechanisms, their physiological relevance is often uncertain. This review examines the recent progress of an alternative, ''top-down'' approach, in which the TME is treated as a holistic biological entity. This approach is made possible by recent advances in systems biology and fabrication technologies that enable the isolation, characterization, and reconstitution of TME. It is hoped that these new techniques will elucidate the nature of niche signals so that they can be extracted, replicated, and controlled. This review summarizes these emerging techniques and how the data they generated are changing our view on TME.

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Rapid and Detergent-Free Decellularization of Cartilage

Cited by 24 publications

References 23 publications

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Recent Advances in Liver Engineering With Decellularized Scaffold

Deconstructing, Replicating, and Engineering Tissue Microenvironment for Stem Cell Differentiation

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