Rapid and direct characterization of total fatty acids in wood by thermochemolysis–gas chromatography–flame ionization detector/mass spectrometry with tetrabutylammonium hydroxide

Mizumoto, Miho; Shimokita, Eisuke; Ona, Toshihiro; Seino, Teruyuki; Ishida, Yasuhiro; Ohtani, Hajime

doi:10.1016/j.jaap.2009.11.004

Cited by 12 publications

(3 citation statements)

References 17 publications

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“…Fatty acid profiles resulting from differences in the basicity of TMAH, TBAH and TMAAc was a foundation of a study for analysis of total fatty acid composition of lipids present in Acacia species [56]. The thermochemolysis-TBAH-GC/MS results were consistent with those obtained using conventional methods of solvent extraction, hydrolysis and derivatization followed by GC/MS.…”

Section: Lipidssupporting

confidence: 53%

Recent applications in analytical thermochemolysis

Shadkami

Helleur

2010

Journal of Analytical and Applied Pyrolysis

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Section: Lipidssupporting

confidence: 53%

Recent applications in analytical thermochemolysis

Shadkami

Helleur

2010

Journal of Analytical and Applied Pyrolysis

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“…Although discussions about the necessity of the derivatization process have lasted for decades, many contradictory opinions are still presented. Regardless of the derivatization process used, the derivatization is always a laborious, tedious and time consuming process, which often leads to lower accuracy and precision [17] . In addition, some interactions between derivatizing agents and active drug compounds are unknown; therefore, further research has been recently carried out on direct determination of fatty acids in various sources without the use of derivatization steps.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a GC–FID method for quantitative analysis of oleic acid and related fatty acids

Zhang

Wang

Liu

2015

Journal of Pharmaceutical Analysis

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Oleic acid is a common pharmaceutical excipient that has been widely used in various dosage forms. Gas chromatography (GC) has often been used as the quantitation method for fatty acids normally requiring a derivatization step. The aim of this study was to develop a simple, robust, and derivatization-free GC method that is suitable for routine analysis of all the major components in oleic acid USP-NF (United States Pharmacopeia-National Formulary) material. A gas chromatography–flame ionization detection (GC–FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material. Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m×0.32 mm i.d.) with a total run time of 20 min. The method was validated in terms of specificity, linearity, precision, accuracy, sensitivity, and robustness. The method can be routinely used for the purpose of oleic acid USP-NF material analysis.

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“…Extractives composition can be determined from natural materials directly (Niemi et al, 2012) and after solvent extraction (Challinor, 1996). Thermochemolysis with TMAH and TMAAc reagents together have been used to determine wood resin acids and fatty acids (Hardell and Nilvebrant, 1999;Mizumoto et al, 2010), and the composition of natural waxes (Asperger et al, 2001). TMAH is used to define total fatty acids, whereas TMAAc defines only the free fatty acids.…”

Section: Extractivesmentioning

confidence: 99%