Rapid and direct speciation of methyltins in seawater by an on-line coupled high performance liquid chromatography–hydride generation–ICP/MS system

Abstract

a b s t r a c tA novel on-line coupled HPLC-hydride generation (HG)-ICP/MS system was developed for rapid, direct and sensitive speciation of methyltins in seawater without any pretreatment step. Methyltin compounds were separated by reversed phase HPLC, and then on-line reacted with potassium borohydride and acetic acid to generate volatile hydride products. The volatile derivatization products were separated in the spray chamber of ICP/MS and then introduced into ICP/MS by argon gas for detection. Monomethyltin (MMT), dimethyltin (DMT) and trimethyltin (TMT) were baseline separated in less than 15 min by reversed phase HPLC. The influence of KBH 4 concentration and type of acid on the system performance was investigated and optimized. Calibration curves, based on peak heights against concentration, were linear in the range of 0.5-50 ng (Sn) mL −1 of methyltins with correlation coefficients of 0.9990, 0.9990 and 0.9996 for MMT, DMT and TMT, respectively. The relative standard deviations measured at 10 ng (Sn) mL −1 for these three methyltins were in the range of 0.6-1.4% (n = 5), and the calculated detection limits (S/N = 3) for MMT, DMT and TMT were 0.266, 0.095 and 0.039 ng (Sn) mL −1 , respectively. This method was successfully applied to the speciation of methyltins in seawater with spiked recovery in the range of 95.4-106.9%. MMT and DMT were detected in all the seawater samples with concentrations in the range of 1.0-1.5 and 0.30-0.57 ng (Sn) mL −1 for MMT and DMT, respectively.

Application of Organometallic Speciation in Clinical Studies

“…Although this method was only applied in the speciation of methyl-Sn compounds in seawater with spiked recoveries in the range of 95.4-106.9%, it is thought that the procedure can be applied also to human samples. The calculated LoDs for MMT, DMT, and TMT were 0.266, 0.095, and 0.039 ng (Sn) mL À1 [182].…”

Fast and effective low‐temperature freezing extraction technique to determine organotin compounds in edible vegetable oil

“…Numerous studies have been reported for the determination of organotin compounds in sediment , bodily fluids , seawater , and marine biota samples . Little has been reported so far on tin speciation in different kinds of edible vegetable oils.…”

Identification of photochemical methylation products of tin(ii) in aqueous solutions using headspace SPME coupled with GC-FPD or GC–MS

“…17 Recent developments in the speciation analysis of tin compounds have been greatly supportive of studies that are involved with their toxicities, metabolism, and environmental behavior. Separation techniques such as gas chromatography (GC) or high performance liquid chromatography (HPLC) have been successfully hyphenated with various detectors that included FPD, [18][19][20] MS, 21,22 or ICP-MS. [23][24][25][26] Prior to tin speciation analysis, sample extraction and derivatization are often necessary, especially for GC separation. Herein, sodium tetraethylborate has been recommended as a good ethylation derivative for liquid samples as it can be directly derivatized in aqueous solutions.…”