Rapid and efficient pesticide detection via cyclodextrin-promoted energy transfer

Abstract

Cyclodextrins facilitate non-covalent fluorescence energy transfer from a variety of pesticides to high quantum-yield fluorophores, resulting in a rapid, sensitive detection scheme for these compounds with detection limits as low as two micromolar.

Detection of bisphenol A and derivatives in human urineviacyclodextrin-promoted fluorescence modulation

“…We demonstrated that this toxicant detection system operates in a broad range of environments, including plasma, 32 breast milk, 33 and urine, 34 as well as in extracts collected in the aftermath of the 2010 Deepwater Horizon oil spill. 35 Although our previous work detected polycyclic aromatic hydrocarbons, 36 polychlorinated biphenyls, 37 aromatic amines, pesticides, 38 aliphatic alcohols, and fuel spill components, we have not used cyclodextrin systems for the detection of BPA and BPA derivatives to date.…”

Towards Rational Chemosensor Design through Improved Understanding of Experimental Parameter Variation and Tolerance in Cyclodextrin-Promoted Fluorescence Detection

“…Such complexes, in turn, effectively limit interactions between the analyte and fluorophore, resulting in a fluorophore emission that is relatively unaffected by the presence of the analyte. For the remainder of the studies, we chose 10 mM cyclodextrin solutions based on previously reported results showing high performance of 10 mM cyclodextrin in promoting energy transfer [19][20][21]23].…”

“…In cases of non-photophysically active analytes, cyclodextrin promotes interactions between the analyte and the fluorophore, resulting in analyte-specific fluorescence modulation [16,17]. We have reported the detection of numerous classes of analytes using these chemosensors, including polycyclic aromatic hydrocarbons (PAHs) [18,19], polychlorinated biphenyls (PCBs) [20], pesticides [21], and aliphatic alcohols [22], as well as the ability of such systems to operate in plasma [23], urine [24], and breast milk [25].…”

Selective detection of non-aromatic pesticides via cyclodextrin-promoted fluorescence modulation

“…Aromatic organochlorine pesticides have already been detected by our group using cyclodextrinpromoted energy transfer to fluorophores 6-8, 27 and a variety of aliphatic alcohols have been detected via cyclodextrinpromoted fluorescence modulation (Table 6). 28 In general, the limits of detection for the alkanols are higher than those of the aliphatic organochlorine pesticides, highlighting a lower degree of sensitivity for these other analytes.…”