1994
DOI: 10.1016/0166-0934(94)90078-7
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Rapid and efficient purification of hepatitis A virus from cell culture

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Cited by 34 publications
(20 citation statements)
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“…Concentrated viral stocks were obtained as previously described (9). Briefly, at 5 to 6 days postinfection, cells from a T-175 cm 2 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40 [pH 7.4]), and incubated for 30 min at room temperature.…”
Section: Virus and Cellsmentioning
confidence: 99%
“…Concentrated viral stocks were obtained as previously described (9). Briefly, at 5 to 6 days postinfection, cells from a T-175 cm 2 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40 [pH 7.4]), and incubated for 30 min at room temperature.…”
Section: Virus and Cellsmentioning
confidence: 99%
“…Concentrated viral stocks were obtained as previously described (4). Briefly, at 5 to 6 days postinfection, cells from a T-175 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40, pH 7.4), and incubated for 30 min at room temperature.…”
Section: Viruses and Cellsmentioning
confidence: 99%
“…The reclassification of HAV was based on a number of differences between HAV and other enteroviruses, including an extreme stability against elevated temperature and low pH (25,26) and a slow and inefficient replicative cycle in cell culture (see references 27 and 31). The uncoating of HAV has been demonstrated to be a slow and asynchronous process (3,5,9,30). By contrast, the attachment to and penetration of cells by HAV appear to be as efficient as for other picornaviruses (3,8,29,32).…”
Section: Human Hepatitis a Virus (Hav) A Member Of The Familymentioning
confidence: 99%
“…1. An African green monkey cell line, BS-C-1, was infected with HAV strain HM175A.2 (1,9) or PV type 1 (Mahoney), and the accumulation of infectious virus within cells was determined after various incubation times at 37°C.…”
Section: Human Hepatitis a Virus (Hav) A Member Of The Familymentioning
confidence: 99%
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