Rapid and efficient purification of chrysophanol in Rheum Palmatum LINN by supercritical fluid extraction coupled with preparative liquid chromatography in tandem

Lo, Tiffany Chien-Ting; Nian, Hung-Chi; Chiu, Kong‐Hwa; Wang, Ai-Yih; Wu, Ben-Zen

doi:10.1016/j.jchromb.2012.02.040

Cited by 22 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anthraquinones of A. vera are bioavailable and therapeutic and have been the subject of numerous studies on cancer and aging (Abdissa, et al, ; Lv, et al, ; Shrestha, Subedi, Chen, & Chang, ). These studies mostly focused on medical aspects such as antioxidant and anticancer properties, and few considered antiglycation activity (Lo, et al, ; Nemmar, et al, ). Our research focused on the inhibition of the formation of AGEs under heated conditions by the main anthraquinones in A. vera .…”

Section: Resultsmentioning

confidence: 99%

“…It is popularly used in functional foods and nutraceuticals (Coelho, et al, ; Sun, et al, ). In A. vera , the chromones show perfect radical scavenging and anti‐inflammatory properties (Choodej, Sommit, & Pudhom, ; Lucini, Pellizzoni, Pellegrino, Molinaria, & Colla, ), and the anthraquinone derivatives show effective antioxidant and anticancer properties (Huang, et al, ; Lo, Nian, Chiu, Wang, & Wu, ; Nemmar, Al‐Salam, Yuvaraju, Beegam, & Ali, ). Many studies of anthraquinones have been used historically for medicinal purposes, numerous studies demonstrated the antioxidant properties, but their potential for the inhibition of AGE formation is not fully known.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Main anthraquinone components in Aloe vera and their inhibitory effects on the formation of advanced glycation end-products

Kang

Zhao

et al. 2016

Journal of Food Processing and Preservation

View full text Add to dashboard Cite

Food heating process causes increased protein glycation and the formation of advanced glycation endproducts (AGEs) which related with diabetes and ageing. Aloe vera (A. vera) extract is a potent antioxidant with established effects attributed largely to a key ingredient anthraquinone. This study investigated the inhibitory ability of the three anthraquinones from A. vera on the formation of AGEs in a heated model system at 70°C. Anthraquinones, including aloe‐emodin (AED), emodin (ED), and chrysophanol (CSN), were extracted from A. vera and analyzed by ultra‐high performance liquid chromatography‐tandem mass spectrometry. Under optimal extraction conditions, the extraction yield of the anthraquinones was 4.377 mg/g. Anthraquinones showed good inhibition toward the formation of glycation products; the inhibition rates of AED, ED, and CSN on crosslinked AGEs were 46.48%, 66.38%, and 57.32% at 10 μM concentrations. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis images also proved that the anthraquinones could significantly inhibit crosslinked AGE formation in a time‐dependent manner. ED showed higher inhibitory ability than AED and CSN on the formation of Nɛ‐(carboxymethyl)lysine and crosslinked AGEs. The results indicate that the antiglycation properties of these anthraquinones are likely related to the phenolic hydroxyl groups in their structures, but this ability is not related to their antioxidant activity, which probably depends on the whole structure of the quinone. Generally, the anthraquinones in A. vera, especially ED, can effectively inhibit AGE formation and reduce protein crosslinking during food processing, even at low concentration. Practical applications The inhibitory effects of three anthraquinones extracted from Aloe vera on the formation of AGEs were investigated in a heated model system. The results indicate Aloe can be used as an effective inhibitor of glycation products in food processing, moreover, this research can be useful for finding suitable nature inhibitors of AGEs for food processing.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Main anthraquinone components in Aloe vera and their inhibitory effects on the formation of advanced glycation end-products

Kang

Zhao

et al. 2016

Journal of Food Processing and Preservation

View full text Add to dashboard Cite

show abstract

“…Results showed that the recovery for chrysophanol in Rheum after SPE–Prep‐HPLC processing was in the range of 88–91.5%. Compared to other extraction and purification methods, SFE–Prep‐HPLC achieved the highest amount of extracted chrysophanol and the shortest run time (3 h) . Juliasih and co‐workers have recently developed a SFE–LC system to determine the microbial community dynamics based on the quinone profile in activated sludge.…”

Section: Sfe With Chromatographic Hyphenated Techniquesmentioning

confidence: 99%

On‐line coupling of supercritical fluid extraction and chromatographic techniques

Sánchez‐Camargo

Parada-Alfonso

Ibáñez

2016

J of Separation Science

View full text Add to dashboard Cite

This review summarizes and discusses recent advances and applications of on-line supercritical fluid extraction coupled to liquid chromatography, gas chromatography, and supercritical fluid chromatographic techniques. Supercritical fluids, due to their exceptional physical properties, provide unique opportunities not only during the extraction step but also in the separation process. Although supercritical fluid extraction is especially suitable for recovery of non-polar organic compounds, this technique can also be successfully applied to the extraction of polar analytes by the aid of modifiers. Supercritical fluid extraction process can be performed following "off-line" or "on-line" approaches and their main features are contrasted herein. Besides, the parameters affecting the supercritical fluid extraction process are explained and a "decision tree" is for the first time presented in this review work as a guide tool for method development. The general principles (instrumental and methodological) of the different on-line couplings of supercritical fluid extraction with chromatographic techniques are described. Advantages and shortcomings of supercritical fluid extraction as hyphenated technique are discussed. Besides, an update of the most recent applications (from 2005 up to now) of the mentioned couplings is also presented in this review.

show abstract

“…Such advantages include the larger contact area values exhibited by theses electrodes, as well as the enhanced mass transport and electrocatalytic effects [64]. Zhang et al [17] proposed a new method for the determination of chrysophanol, an anti-inflammatory and antimicrobial compound [65], based on the use of a GCE modified with ABNPs dispersed in a DHP film. A significantly increased peak current was detected for chrysophanol oxidation using the ABNPs-DHP/GCE system, in comparison to the bare GCE electrode.…”

Section: Nanoparticles-containing Electrochemical Sensorsmentioning

confidence: 99%

The use of dihexadecylphosphate in sensing and biosensing

Janegitz

Baccarin

Raymundo‐Pereira

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Rapid and efficient purification of chrysophanol in Rheum Palmatum LINN by supercritical fluid extraction coupled with preparative liquid chromatography in tandem

Cited by 22 publications

References 28 publications

Main anthraquinone components in Aloe vera and their inhibitory effects on the formation of advanced glycation end-products

Main anthraquinone components in Aloe vera and their inhibitory effects on the formation of advanced glycation end-products

On‐line coupling of supercritical fluid extraction and chromatographic techniques

The use of dihexadecylphosphate in sensing and biosensing

Contact Info

Product

Resources

About