Rapid and Efficient Recovery of Histidine‐tagged Proteins Directly from Bacterial Cultures

New membrane systems for the purification of proteins are presented. These disposable devices enable time saving screenings of downstream conditions with a small amount of protein solution. Furthermore, scaling up to the production level is directly possible since devices with effective membrane surfaces from 10 cm2 to 1 m2 are available. Several membranes like ion exchange membranes were investigated for the determination of protein binding capacities. Metal chelate membranes were used for ligand binding, which enables the determination of the membrane systems potential for affinity chromatography. Lysozyme and BSA are chosen for the determination of binding capacities of ion exchange membranes. Furthermore, Con A membranes, suitable for the isolation of glycoproteins, were developed and their binding properties were tested.

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Fast Screening for the Purification of Proteins Using Membrane Adsorber Technology

Harkensee

Kökpınar

Walter

et al. 2007

Engineering in Life Sciences

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Rapid and Efficient Recovery of Histidine‐tagged Proteins Directly from Bacterial Cultures

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Fast Screening for the Purification of Proteins Using Membrane Adsorber Technology

Fast Screening for the Purification of Proteins Using Membrane Adsorber Technology

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