Rapid and feasible TLC screening of tea tree oil commercial samples

Abstract

Tea tree oil is an essential oil used for medical and health purposes consisting of a complex mixture of organic compounds present in high or trace amounts. Several factors (i.e. chemotype, environmental conditions, preparation procedures) can affect the composition and consequently the biological properties, therefore, quality assurance is a key requisite to guarantee reproducibility among batches. Compliance with ISO standards by gas-chromatography mass-spectrometry techniques are universally accepted in check of tea tree oil formulations, while adulterations might be overlooked. In this study we set a TLC protocol for the easy and cheap evaluation of tea tree oil batches purchased from the marketplace and, coupled to the European Pharmacopoeia assay of fatty oils, for the detection of adulterations. This protocol aims to be a procedure for the quality evaluation of tea tree oil in small laboratories not equipped with expensive analytical instruments and by health professionals dealing with herbal medicines.

Algorithm for the development of a multicomponent pharmaceutical substance of plant origin with antimicrobial action: from science search to dosage form

“…Определение антимикробного взаимодействия соединений в субстанции методом шахматной доски [38]. Показателем характера совместного антимикробного действия веществ являлся индекс фракционной ингибирующей концентрации (иФИК), который рассчитывали по формуле [39].…”

“…Подбор подвижных фаз для аналитической методики ТСХ осуществляли на основе научных данных о ТСХ многокомпонентных субстанций и их индивидуальных соединений [39,40] В субстанции превалируют монотерпены и различные терпеноиды (таблица 2). β-Бисаболол, фарнезол и транс-геранилгераниол являются родственными примесями α-(-)-бисаболола [35].…”

Pterodon emarginatus Seed Preparations: Antiradical Activity, Chemical Characterization, and In Silico ADMET Parameters of β-caryophyllene and Farnesol

“…Methanol was used to dissolve all extracts at a concentration of 5 mg/mL, while essential oil was solubilized at 5 mg/L. A precise aliquot of 1,3,5-triisopropylbenzene, a volatile compound that is certainly not present in the natural extracts, is added to the samples to be injected as an internal standard (IS) [46,47]. β-Caryophyllene and farnesol were quantified by calibration curves, which were experimentally constructed with correlation coefficients close to unity.…”