Rapid and Highly Efficient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant

Tan, Xiao; Chen, Shuang; Fang, Yang; Liu, Penghui; Hu, Zhubin; Jin, Yanling; Yi, Zhuolin; He, Kai; Li, Xing; Zhao, Leyi; Wang, Honggang; Zhao, Hai

doi:10.3390/biom12121881

Cited by 6 publications

(2 citation statements)

References 79 publications

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“…The vectors of the 35S:eGFP and the LpSUT2:eGFP were introduced into duckweed’s callus following Agrobacterium tumefaciens strain GV3101-mediated transformation. Then, follow the previously established method to screen and regenerate these calluses ( Tan et al., 2022 ). Briefly, put these callus in MS medium (screening 6 weeks) and 1/2 Schenk and Hildebrandt (SH) medium (regeneration 4-5 weeks).…”

Section: Methodsmentioning

confidence: 99%

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Wei,

Hu,

Wang

et al. 2024

Front. Plant Sci.

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Promoters are one of the most critical elements in regulating gene expression. They are considered essential biotechnological tools for heterologous protein production. The one most widely used in plants is the 35S promoter from cauliflower mosaic virus. However, our study for the first time discovered the 35S promoter reduced the expression of exogenous proteins under increased antibiotic stress. We discovered an endogenous strong promoter from duckweed named LpSUT2 that keeps higher initiation activity under antibiotic stress. Stable transformation in duckweed showed that the gene expression of eGFP in the LpSUT2:eGFP was 1.76 times that of the 35S:eGFP at 100 mg.L-1 G418 and 6.18 times at 500 mg.L-1 G418. Notably, with the increase of G418 concentration, the gene expression and the fluorescence signal of eGFP in the 35S:eGFP were weakened, while the LpSUT2:eGFP only changed slightly. This is because, under high antibiotic stress, the 35S promoter was methylated, leading to the gene silencing of the eGFP gene. Meanwhile, the LpSUT2 promoter was not methylated and maintained high activity. This is a previously unknown mechanism that provides us with new insights into screening more stable promoters that are less affected by environmental stress. These outcomes suggest that the LpSUT2 promoter has a high capacity to initiate the expression of exogenous proteins. In conclusion, our study provides a promoter tool with potential application for plant genetic engineering and also provides new insights into screening promoters.

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Section: Methodsmentioning

confidence: 99%

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Wei,

Hu,

Wang

et al. 2024

Front. Plant Sci.

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“…Moreover, the group fed with chIL-17B transgenic plants exhibited significantly higher IBV-specific antibody titers and increased secretory immunoglobulin A (sIgA) concentrations. This approach enhances the immunological applications of duckweed, making it a viable platform for producing medical therapeutics [ 63 ]. In the area of biofuel production, L. japonica was successfully engineered to accumulate triacylglycerols by modifying lipid biosynthesis pathways.…”

Section: Transgenic Development Of Duckweedmentioning

confidence: 99%

Duckweed: Beyond an Efficient Plant Model System

Thingujam,

Pajerowska-Mukhtar,

Mukhtar

2024

Biomolecules

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Duckweed (Lemnaceae) rises as a crucial model system due to its unique characteristics and wide-ranging utility. The significance of physiological research and phytoremediation highlights the intricate potential of duckweed in the current era of plant biology. Special attention to duckweed has been brought due to its distinctive features of nutrient uptake, ion transport dynamics, detoxification, intricate signaling, and stress tolerance. In addition, duckweed can alleviate environmental pollutants and enhance sustainability by participating in bioremediation processes and wastewater treatment. Furthermore, insights into the genomic complexity of Lemnaceae species and the flourishing field of transgenic development highlight the opportunities for genetic manipulation and biotechnological innovations. Novel methods for the germplasm conservation of duckweed can be adopted to preserve genetic diversity for future research endeavors and breeding programs. This review centers around prospects in duckweed research promoting interdisciplinary collaborations and technological advancements to drive its full potential as a model organism.

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Screening potential antileukemia agents from duckweed: Integration of chemical profiling, network pharmacology, and experimental validation

Liu,

Huang,

Yang

et al. 2024

Phytochemical Analysis

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IntroductionThe identification of active dietary flavonoids in food is promising for novel drug discovery. The active ingredients of duckweed (a widely recognized food and herb with abundant flavonoids) that are associated with acute myeloid leukemia (AML) have yet to be identified, and their underlying mechanisms have not been elucidated.ObjectivesThe objective of this study was to identify novel constituents exhibiting antileukemia activity in duckweed through the integration of chemical profiling, network pharmacology, and experimental validation.MethodsFirst, high performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) was used to characterize the primary constituents of duckweed. Subsequently, AML cell‐xenograft tumor models were used to validate the anticancer effect of duckweed extract. Furthermore, network pharmacology analysis was conducted to predict the potential active compounds and drug targets against AML. Lastly, based on these findings, two monomers (apiin and luteoloside) were selected for experimental validation.ResultsA total of 17 compounds, all of which are apigenin and luteolin derivatives, were identified in duckweed. The duckweed extract significantly inhibited AML cell growth in vivo. Furthermore, a total of 88 targets for duckweed against AML were predicted, with key targets including PTGS2, MYC, MDM2, VEGFA, CTNNB1, CASP3, EGFR, TP53, HSP90AA1, CCND1, MMP9, TNF, and MAPK1. GO and KEGG pathway enrichment analyses indicated that these targets were primarily involved in the apoptotic signaling pathway. Lastly, both apiin and luteoloside effectively induced apoptosis through CASP3 activation, and this effect could be partially reversed by a caspase inhibitor (Z‐VAD).ConclusionDuckweed extract has an antileukemic effect, and apiin derived from duckweed shows potential as a treatment for AML.

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Rapid and Highly Efficient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant

Cited by 6 publications

References 79 publications

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Duckweed: Beyond an Efficient Plant Model System

Screening potential antileukemia agents from duckweed: Integration of chemical profiling, network pharmacology, and experimental validation

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