Rapid and label-free detection of Aflatoxin-B1 via microfluidic electrochemical biosensor based on manganese (III) oxide (Mn₃O₄) synthesized by co-precipitation route at room temperature

Abstract

Aflatoxin B1 (AFB1) is the most toxic mycotoxin, naturally occurring in food items, and it causes several types of lethal diseases. Therefore, a rapid and convenient detection method for AFB1 is the first step towards overcoming the effect of AFB1. The current work presents the development of an efficient microfluidic electrochemical-based biosensor using tri-manganese tetroxide nanoparticles (Mn3O4 nps) for AFB1 detection. The Mn3O4 nps were synthesized at room temperature through the co-precipitation route. Its phase purity, structural and morphological studies have been characterized through the X-ray diffraction, Raman spectroscopy, energy-dispersive X-ray, Fourier transform infrared spectroscopy, and transmission electron microscopy. The mask-less UV-lithography was carried out to fabricate the three-electrode chip and microfluidic channel of the microfluidic electrochemical biosensing system. The designed microfluidic immunosensor (BSA/Ab-AFB1/Mn3O4/ITO) was fabricated using the three-electrode chip, microfluidic channel in poly-dimethyl siloxane. The fabricated sensor exhibited the 3.4 µA mL ng-1 cm-2 sensitivity and had the lowest lower detection limit of 0.295 pg mL-1 with the detection range of 1 pg mL-1 to 300 ng mL-1. Additionally, the spiked study was also performed with this immunoelectrode and a recovery rate was obtained of 108.2 %.

Enhanced Photocatalytic Degradation of <i>p</i>-Nitrophenol and Phenol Red Through Synergistic Effects of a CeO₂-TiO₂ Nanocomposite

“…The average crystallite size was calculated using the Debye-Scherrer equation as explained in our previously published paper. The average crystallite size was 7.15 and 6.25 nm for TiO 2 and CeO 2 , respectively [19,42]. Figure 2 shows the UV-Vis absorption plot for CeO 2 /TiO 2 nc.…”

Interaction Studies of PVP and CTAB Capped CuO Nanorods with Aldicarb and Chlorpyrifos

“…com ECS Journal of Solid State Science and Technology, 2024 13 037006 CuO) were synthesized by the co-precipitation method as described in previously studied literature. [35][36][37] The precursor was high purity copper sulphate (Merck), and reducing agent used here was sodium hydroxide (NaOH). Using these precursors, 0.25 M of copper sulphate and 0.1 M of NaOH solutions were prepared separately.…”

Graphene oxide-Mn3O4 nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

“…Synthesis of Mn 3 O 4 nanoparticles. The Mn 3 O 4 nps were synthesized using the co-precipitation method [31]. In the typical synthesis, 0.2 M of ((CH 3 COO) 2 Mn.4H 2 O) and 0.5 M of NaOH were used as precursors.…”