Rapid and label-free fluorescence bioassay for microRNA based on exonuclease III-assisted cycle amplification

Abstract

A new assay for the rapid and simple detection of microRNA based on G-quadruplex and Exonuclease III (ExoIII) dual signal amplification was constructed.

Direct and accurate miRNA detection based on CRISPR/Cas13a-triggered exonuclease-iii-assisted colorimetric assay

“…However, it has only limited effectiveness on single-stranded DNA or doublestranded DNA with a projecting 3'-end. Exo-iii facilitates target regeneration without the need for a specific recognition site, distinguishing it from nicking endonuclease (Liu et al 2018;Huang et al 2014). This characteristic renders the Exo-iii-assisted approaches an adaptable and potent amplification strategy suitable for a wide range of biosensing applications.…”

A one-step fluorescent biosensing strategy for highly sensitive detection of HIV-related DNA based on strand displacement amplification and DNAzymes

“…14 Among these methods, uorescent method usually takes precedence as an alternative platform owing to its advantages of high sensitivity and simple procedure. To further improve the performance of the biosensors, different isothermal amplication strategies have been developed for genetic test and medical diagnosis, such as rolling circle amplication (RCA), [15][16][17][18] hybridization chain reaction (HCR), 19,20 catalytic hairpin assembly (CHA), 21,22 exonuclease III aided signal amplication, 23,24 and strand displacement amplication (SDA). 25 Combining polymerasemediated strand extension with nicking enzyme-assisted single strand cleaving process, SDA is an isothermal molecular chain reaction with high amplication efficiency.…”