Rapid and low-cost identification of common sole (Solea solea) in the field using a fast DNA isolation protocol and loop-mediated isothermal amplification (LAMP)

Deconinck, Dumas; Robbens, Johan; Volckaert, Filip; Derycke, Sophie

doi:10.1016/j.jfca.2023.105166

Cited by 14 publications

(5 citation statements)

References 31 publications

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“…45 For the P. platessa primer set, only a LB primer could be designed, which could explain the slightly lower LOD of the P. platessa assay. Compared to the recently published results of Deconinck et al, 31 the LOD of the S. solea assay of this study is lower than 0.1 ng because instead of a colorimetric readout, a more sensitive method has been used. To sum up, the P. platessa specific LAMP assay detected up to 0.1 ng of target DNA, and the S. solea specific LAMP assay detected 0.01 ng.…”

Section: ■ Results and Discussioncontrasting

confidence: 65%

“…platessa assay. Compared to the recently published results of Deconinck et al, the LOD of the S. solea assay of this study is lower than 0.1 ng because instead of a colorimetric readout, a more sensitive method has been used.…”

Section: Resultscontrasting

confidence: 61%

“…Both platforms have the potential to enable in-field detection of fish species and thus contribute to compliance with current legislation and extend already existing in-field readout systems for Pleuronectiformes. 31 ■ MATERIALS AND METHODS Sample Material. Raw or frozen, whole, or filleted fish samples as well as fish samples in ethanol were obtained from local farmer's markets or local producers or had been collected in previous projects (Table 1).…”

Section: ■ Introductionmentioning

confidence: 99%

“…The isothermal lab-on-phone assay offers the advantage of real-time monitoring, simple and user-friendly operation, and more informative results than LFAs. Both platforms have the potential to enable in-field detection of fish species and thus contribute to compliance with current legislation and extend already existing in-field readout systems for Pleuronectiformes …”

Section: Introductionmentioning

confidence: 99%

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Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Wax,

Pförtner,

Holz

et al. 2023

J. Agric. Food Chem.

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The detection of a Cytochrome b gene (cytb) for species differentiation in fish is intensively used. A fast alternative to expensive and time-consuming DNA barcoding is loop-mediated isothermal amplification (LAMP) in combination with efficient readout systems. For this reason, we developed LAMP assays for rapid species detection of Pleuronectes platessa and Solea solea, two economically important flatfish species in Europe that are prone to mislabeling. Species-specific primer sets targeting cytb were designed, and LAMP assays were optimized. With the optimized LAMP assays, we were able to detect up to 0.1 and 0.01 ng of target DNA of P. platessa and S. solea, respectively, and in each case up to 1% (w/w) of target species in mixtures with nontarget species. For future on-site detection, a lateral flow assay and a pocket-sized lab-on-phone assay were used as readout systems. The lab-on-phone assay with the S. solea specific primer set revealed cross-reactivity to Solea senegalensis. The assay targeting P. platessa proved to be highly specific. Both assays could be performed within 45 min and provided rapid and easy detection of fish species.

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Section: ■ Results and Discussioncontrasting

confidence: 65%

Section: Resultscontrasting

confidence: 61%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Wax,

Pförtner,

Holz

et al. 2023

J. Agric. Food Chem.

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“…While the PCR method has been used for fish identification for a long time, the LAMP method is still used more for the detection of fish parasites and other microorganisms. 49−52 To the best of our knowledge, the use of LAMP for the identification of the fish itself has been published only for the members of salmon, 53,54 trout, 55 tuna, 29,56 eel, 57 cod, 58 common sole, 59 Arothorn or Diodon genus. 60,61 Thus, we believe that the present paper is the first to propose a LAMP protocol for pike species identification and, moreover, one of the few that use a nuclear gene as a marker since most published protocols have focused on mitochondrial cytochrome b.…”

Section: Gcagaa[+c]tttaa) and Shorter Probe Esox_p3 With Even Modific...mentioning

confidence: 99%

Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment

Čermáková,

Mukherjee,

Nováková

et al. 2024

J. Agric. Food Chem.

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Fish from the pike (Esox) genus are valued in gastronomy for their superior meat quality. However, they can cause allergic reactions in sensitive consumers. This work aimed to fill the gap in the detection of pike allergens using molecular–biological techniques. New, fast, and accurate loop-mediated isothermal amplification (LAMP) and real-time PCR (qPCR) assays were designed to detect pike DNA using the parvalbumin gene as a marker. LAMP was assessed by electrophoresis, SYBR green optical detection, and real-time fluorescence detection. The latter was the most sensitive, detecting as little as 0.78 ng of pike DNA; the qPCR detection limit was 0.1 ng. The LAMP analysis took 20–70 min, which is significantly faster than qPCR. The study provides reliable detection and quantification of the parvalbumin gene in both fresh and processed samples and further highlights the versatility of the use of the parvalbumin gene for the authentication of food products and consumer protection via refined allergen risk assessment that is independent of the type of tissue or food processing method used.

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Rapid and Portable Presumptive Loop‐Mediated Isothermal Amplification Assays for the Detection of the Invasive Corn Snake (Pantherophis guttatus)

Deliveyne,

Austin,

Sanders

et al. 2024

Environmental DNA

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The exotic pet trade is a major pathway for the introduction, establishment, and spread of novel invasive alien species. Reptiles are common in the exotic pet trade and are prominent invasive alien vertebrate species that have dire impacts if allowed to establish. The North American corn snake (Pantherophis guttatus) is particularly common in the international pet trade and has been identified as a vertebrate pest priority species in Australia due to widespread climate suitability and prevalence in pre‐ and post‐border seizure records. Consequently, rapid, and presumptive post‐border biosecurity detection is essential to prevent its establishment and spread. Loop‐mediated isothermal amplification (LAMP) is an emerging biosecurity tool that has shown promise for rapid detection of several high‐risk species. We developed two LAMP assays for the detection of P. guttatus, validated against: synthetic DNA; DNA extracted from snap‐frozen tissue, and shed skins; and then compared their performance for the detection of trace DNA collected from swabs of glass tanks post reptile presence. Our results include laboratory optimization and assessment of two mobile devices for in‐field integration (Franklin Real‐Time PCR Thermocycler, Biomeme, USA, and Genie III, Optigene, UK). The results indicate that LAMP is a viable biosecurity tool, with DNA detection possible for a range of sample types in a total of c.30 min, when including a rapid extraction step (8 min). Herein, we provide tools for rapid, presumptive detection of the North American corn snake from trace DNA samples in Australian biosecurity and wildlife compliance settings.

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Rapid and low-cost identification of common sole (Solea solea) in the field using a fast DNA isolation protocol and loop-mediated isothermal amplification (LAMP)

Cited by 14 publications

References 31 publications

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment

Rapid and Portable Presumptive Loop‐Mediated Isothermal Amplification Assays for the Detection of the Invasive Corn Snake (Pantherophis guttatus)

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