1983
DOI: 10.1016/0002-9343(83)90082-7
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Rapid and reliable techniques for the laboratory detection of bacterial meningitis

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1983
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Cited by 21 publications
(7 citation statements)
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“…Bacterial colonies grown from blood and CSF specimens underwent identification using standard biochemical test methods [10]. All bacterial isolates (stored in skim milk), blood culture supernatant specimens, and CSF specimens were shipped in labeled cryotubes at Ϫ80ЊC to the Molecular Biology Laboratory of the Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan, for testing by PCR.…”
Section: Study Population and Hospitalmentioning
confidence: 99%
“…Bacterial colonies grown from blood and CSF specimens underwent identification using standard biochemical test methods [10]. All bacterial isolates (stored in skim milk), blood culture supernatant specimens, and CSF specimens were shipped in labeled cryotubes at Ϫ80ЊC to the Molecular Biology Laboratory of the Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan, for testing by PCR.…”
Section: Study Population and Hospitalmentioning
confidence: 99%
“…At the same time, these broad-spectrum antibiotics should not be administered without need: antimicrobial-resistant meningitis, including meropenem-resistant Streptococcus pneumoniae in the CSF, has already been isolated, and its spread in Japan and Taiwan is a cause for concern [4,5]. Distinguishing bacterial meningitis from aseptic meningitis, which is caused mainly by viral infection, is therefore crucial, but is challenging because the symptoms are often similar [3,6]. There is no single laboratory test that adequately discriminates between bacterial and aseptic meningitis [7,8].…”
Section: Introductionmentioning
confidence: 99%
“…Gram stain of the cerebrospinal uid (CSF) is a rapid, inexpensive, and accurate method for detecting the etiologic agents of bacterial meningitis [9,6]. The sensitivity of CSF Gram staining is 60-98%, depending on the patient population, type of bacteria, and previous antimicrobial treatment, and its speci city is nearly 100% [9,10], [11,12], [13], [14,15], [16,17].…”
Section: Introductionmentioning
confidence: 99%
“…Electrophoresis is a simple and fast technique, but only semi-quantitative due to its limited resolution. Moreover, discrimination among amplification products with similar lengths using electrophoresis is difficult [7]. …”
Section: Introductionmentioning
confidence: 99%