Rapid and reproducible analysis of thiocyanate in real human serum and saliva using a droplet SERS-microfluidic chip

Wu, Lei; Wang, Zhuyuan; Zong, Shenfei; Cui, Yiping

doi:10.1016/j.bios.2014.06.026

Cited by 108 publications

(67 citation statements)

References 50 publications

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“…Currently, we are also working on integrating the SERS-based exosome detection method with microuidic chips. Considering our previous experience in microuidic-based biosensing, 30,31 we believe that we can get better results in future works.…”

Section: Exosome Detection Using the Magnetic Nanobeads And Sers Nanomentioning

confidence: 94%

Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes

et al. 2016

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Exosomes play an important role in intercellular communications. Here, we present a surface-enhanced Raman scattering (SERS) based detection method for tumor-derived exosomes using SERS nanoprobes and magnetic nanobeads. The SERS nanoprobe has a core-shell structure, with gold core-silver shell nanorods (Au@Ag NRs) as the SERS active core, Raman molecules as the SERS reporter and a silica layer as the protecting shell. The outmost surface of the SERS nanoprobe is decorated with exosome-specific antibodies. Each magnetic nanobead is fabricated by coating Fe 3 O 4 nanoparticles (NPs) with a silica shell and attaching specific antibodies to the silica shell. With target exosomes present, the magnetic nanobeads and SERS nanoprobes can capture the exosomes by forming a sandwich-type immunocomplex. The immunocomplex (as well as the SERS nanoprobes) can be precipitated with a magnet, and thus SERS signals can be detected in the precipitates. With no target exosomes present, no immunocomplex can be formed, and thus quite weak SERS signals will be detected in the precipitates. Hence, the SERS signal of the final magnetic separation product can be used to detect exosomes. In the experiment, using one kind of tumor cells and one kind of normal cells, we proved that the presented method can be used for both qualitative and quantitative detection of tumor-derived exosomes.

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Section: Exosome Detection Using the Magnetic Nanobeads And Sers Nanomentioning

confidence: 94%

Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes

et al. 2016

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“…In this way, Wu et al detected thiocyanate in real human serum and saliva using Au@Ag core-shell nanorods. They found that, with increasing the flow periods for the mixed solution through the winding channel, the SERS signals increased before reaching an equilibrium value [39]. That is, the degree of mixing and interaction between the noble metal nanostructure and analyte solution were crucial to SERS detection.…”

Section: Nanostructure Colloids As Sers-sensitive Elementsmentioning

confidence: 98%

“…The effective dispersion and mixing of nanostructures can, to some extent, prevent the unwanted aggregation of nanostructures and improve the uniformity of the adsorption of the analyte molecules on the NPs. This improves the reproducibility of the sensors [39]. For SERS devices using inset regular nanostructure arrays as substrates, reproducibility is assured by the nanostructure arrays [41].…”

Section: Nanostructure Colloids As Sers-sensitive Elementsmentioning

confidence: 98%

Review of microfluidic approaches for surface-enhanced Raman scattering

Zhou

Kim

2016

Sensors and Actuators B: Chemical

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“…[95] Rapid and reproducible analysis of thiocyanate in real human serum and saliva by using a droplet SERS-microfluidic chip Human serum and saliva The reaction required less than 15 s in the designed channel, which is at least 40-fold shorter than that for solid metallic substrates. [96] A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing Escherichia coli O157 The immunocapture efficiency was 85%-92%, higher than 44%-86% of offline immunomagnetic separation. [97] Rapid detection of bacteriophages in starter culture using water-in-oil-in-water emulsion microdroplets…”

Section: Human Immunodeficiency Virus Variola Virusmentioning

confidence: 99%

Recent Advances in Applications of Droplet Microfluidics

et al. 2015

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Droplet-based microfluidics is a colloidal and interfacial system that has rapidly progressed in the past decade because of the advantages of low fabrication costs, small sample volumes, reduced analysis durations, high-throughput analysis with exceptional sensitivity, enhanced operational flexibility, and facile automation. This technology has emerged as a new tool for many recently used applications in molecular detection, imaging, drug delivery, diagnostics, cell biology and other fields. Herein, we review recent applications of droplet microfluidics proposed since 2013.

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Rapid and reproducible analysis of thiocyanate in real human serum and saliva using a droplet SERS-microfluidic chip

Cited by 108 publications

References 50 publications

Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes

Facile detection of tumor-derived exosomes using magnetic nanobeads and SERS nanoprobes

Review of microfluidic approaches for surface-enhanced Raman scattering

Recent Advances in Applications of Droplet Microfluidics

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