Rapid and selective detection of biothiols by novel ruthenium(II) complex-based phosphorescence probes

Zheng, Ze-Bao; Han, Yinfeng; Ge, Yanqing; Cui, Jichun; Zuo, Jian; Nie, Kun

doi:10.1016/j.saa.2019.03.067

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…Notably, the photocytotoxicity of the ruthenium(II)-containing supramolecular assemblies is higher in intracellular regions as the photogenerated 1 O 2 causes direct oxidative damage to proximal organelles instead of diffusing across the plasma membrane or extracellular space, indicating the importance of cellular localization in determining their photocytotoxic activity. Iridium(III) complexes featuring two tetrazine units (697) have also been developed as phosphorogenic bioorthogonal probes for bis-cyclooctynylated substrates. 1296 These complexes show extremely weak emission (Φ em = 0.0001 to 0.0009) and 1 O 2 generation (Φ Δ = 0.02 to 0.07).…”

Section: Inverse Electron-demand Diels−alder Reactionmentioning

confidence: 99%

“…The complex is transported to their body through feeding, and its reaction with biothiols present in the digestive system results in intense emission in the esophagus and gut regions. Zheng, Ge, and co-workers have utilized 2,4-DNP-functionalized mono- and binuclear ruthenium(II) complexes such as 384 and 385 that exhibit rapid and selective emission turn-on in response to biothiols for imaging endogenous biothiols in live Glioma cells. The steric interactions between the 1 H -imidazo[4,5- f ][1,10]phenanthroline ligand and the 2,4-DNP moiety facilitate the reaction with thiols, allowing the reaction to complete within 10 min.…”

Section: Luminescent Transition Metal Complexes As Probes For Biologi...mentioning

confidence: 99%

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Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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Luminescence imaging is a powerful and versatile technique for investigating cell physiology and pathology in living systems, making significant contributions to life science research and clinical diagnosis. In recent years, luminescent transition metal complexes have gained significant attention for diagnostic and therapeutic applications due to their unique photophysical and photochemical properties. In this Review, we provide a comprehensive overview of the recent development of luminescent transition metal complexes for bioimaging and biosensing applications, with a focus on transition metal centers with a d 6 , d 8 , and d 10 electronic configuration. We elucidate the structure−property relationships of luminescent transition metal complexes, exploring how their structural characteristics can be manipulated to control their biological behavior such as cellular uptake, localization, biocompatibility, pharmacokinetics, and biodistribution. Furthermore, we introduce the various design strategies that leverage the interesting photophysical properties of luminescent transition metal complexes for a wide variety of biological applications, including autofluorescence-free imaging, multimodal imaging, organelle imaging, biological sensing, microenvironment monitoring, bioorthogonal labeling, bacterial imaging, and cell viability assessment. Finally, we provide insights into the challenges and perspectives of luminescent transition metal complexes for bioimaging and biosensing applications, as well as their use in disease diagnosis and treatment evaluation.

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Section: Inverse Electron-demand Diels−alder Reactionmentioning

confidence: 99%

Section: Luminescent Transition Metal Complexes As Probes For Biologi...mentioning

confidence: 99%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

View full text Add to dashboard Cite

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“…Recent studies have also revealed that the concentration of biothiols is implicated in different conditions, such as inflammation, cardiovascular diseases, HIV infection and cancers [161]. To detect biothiols in the body, a series of Ru(II) complex chemosensors have been developed through the following response mechanisms, (1) nucleophilic substitution and cleavage of the sulfonamide or sulfonate ester bond [212][213][214], (2) cyclization of aldehyde group with amino and thiol groups [100,215,216] and (3) others [217], such as reaction with azo group [218] and α,β-unsaturated ketone [219], cleavage of NBD and displacement of metal ions [139,220].…”

Section: Ru(ii) Complex Chemosensors For Biothiolsmentioning

confidence: 99%

Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex-Based Chemosensors

Zhang

Yong

et al. 2022

Metal Ligand Chromophores for Bioassays

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Luminescence chemosensors are one of the most useful tools for the determination and imaging of small biomolecules and ions in situ in real time. Based on the unique photo-physical/-chemical properties of ruthenium(II) (Ru(II)) complexes, the development of Ru(II) complex-based chemosensors has attracted increasing attention in recent years, and thus many Ru(II) complexes have been designed and synthesized for the detection of ions and small biomolecules in biological and environmental samples. In this work, we summarize the research advances in the development of Ru(II) complex-based chemosensors for the determination of ions and small biomolecules, including anions, metal ions, reactive biomolecules and amino acids, with a particular focus on binding/reaction-based chemosensors for the investigation of intracellular analytes’ evolution through luminescence analysis and imaging. The advances, challenges and future research directions in the development of Ru(II) complex-based chemosensors are also discussed.

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“Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo

Liu

Zhang

et al. 2020

Advanced Science

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In this work, a “two birds with one stone” ruthenium(II) complex probe, Ru‐NBD, is proposed as an effective tool for biothiols detection and discrimination in vitro and in vivo. Ru‐NBD is nonluminescent due to the quenching of Ru(II) complex emission by photoinduced electron transfer (PET) from Ru(II) center to NBD and the quenching of NBD emission through 4‐substitution with “O” ether bond. Ru‐NBD is capable of reacting with Cys/Hcy to form long‐lived red‐emitting Ru‐OH and short‐lived green‐emitting NBD‐NR, while reacting with GSH to produce Ru‐OH and nonemissive NBD‐SR. The long lifetime emission of Ru(II) complex allows elimination of short lifetime background and NBD‐NR fluorescence for total biothiols detection (“bird” one) by time‐gated luminescence (TGL) analysis, and the remarkable difference in luminescence color response allows discrimination GSH and Cys/Hcy (“bird” two) through steady‐state luminescence analysis. Ru‐NBD features high sensitivity and selectivity, rapid luminescence response, and low cytotoxicity, which enables it to be used as the probe for luminescence and background‐free TGL detection and visualization of biothiols in live cells, zebrafish, and mice. The successful development of this probe is anticipated to contribute to the future biological studies of biothiols roles in various diseases.

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Rapid and selective detection of biothiols by novel ruthenium(II) complex-based phosphorescence probes

Cited by 8 publications

References 33 publications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex-Based Chemosensors

“Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo

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