Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cao, Lei; Li, C.

doi:10.1556/achrom.24.2012.3.4

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…Then, centrifugation at 8000 rpm for 15 min and then the supernatant liquid was collected and finally filtered through a 0.45 μ millipore membrane filter. [13] The resultant plasma solution (200 μT) followed by adding 10 μ0 of internal standard and then 20 μl of the final mixture (210 μo) was injected into the HPLC column.…”

Section: Sample Treatmentmentioning

confidence: 99%

“…[13] There are several small unknown peaks of plasma and the retention time of an unknown peak was near to the baclofen peak that not affect the separation. To make them far separated, the separation was studied carefully by increasing the content of methanol in methanol-water mixture from 50% to 55% and using a column of 300 mm instead of 200 mm.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…Analysis was run at a flow rate of 2.0 ml/min for 10 minutes, the column was kept at room temperature, and the detection wavelength was 220 nm. [13] …”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…The method was fully validated with respect to adequate sensitivity, linearity, recovery, accuracy, and precision (within-day and between-day). [13] …”

Section: Samples Preparationmentioning

confidence: 99%

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Ali¹

2017

AJP

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Aim: The aim of this study to compare the relative bioavailability of baclofen as a peroral tablet with the new formulations (transdermal and buccal films). Materials and Methods: Rapid and simple high-performance liquid chromatography/ultraviolet method has been adopted and validated for the determination of baclofen in human plasma with acceptable linearity, precision and accuracy. The study was performed on six human volunteers divided into three groups each contained two individuals. The first group was dosed with 20 mg of baclofen tablet (Lioraz ® ) as a reference, the second group received 40 mg baclofen transdermal film, and the third group received 20 mg baclofen buccal film. Transdermal and buccal films were subjected to in vivo study to evaluate the bioavailability parameters and then compared to the reference. Results and Discussion: Following Lioraz ® tablet, the maximum concentration was achieved after 3.0 h unlike, after transdermal film with a double dose, it was at 6.0 h whereas, following buccal film, maximum time was 3.0 h post-dosing. Thus absorption from buccal films was faster, whereas transdermal spent longer times to reach the maximum concentration in systemic circulation. The mean values of pharmacokinetic parameters were significantly higher from transdermal than oral, demonstrating prolongation of baclofen action, whereas with buccal was lower. Taking Lioraz ® as a reference, the % relative bioavailability from transdermal and buccal film was 78.99% and 89.22%, respectively. Conclusion: Study indicates that the absorption from buccal films was faster, whereas the transdermal spent longer times to reach the maximum drug concentration. Transdermal film of baclofen was successful in all the established studies by improving the prolongation of action and increasing the half-life of baclofen.

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Section: Sample Treatmentmentioning

confidence: 99%

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…Analysis was run at a flow rate of 2.0 ml/min for 10 minutes, the column was kept at room temperature, and the detection wavelength was 220 nm. [13] …”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…The method was fully validated with respect to adequate sensitivity, linearity, recovery, accuracy, and precision (within-day and between-day). [13] …”

Section: Samples Preparationmentioning

confidence: 99%

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Ali¹

2017

AJP

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“…To date, Bac has been determined in various biological materials using techniques based on gas (Giovanni & d'Aloja, ; Kochak & Hunc, ) or liquid (Ban, Park, & Kim, ; Fraser, MacNeil, & Isner, ; Tosunoglu & Ersoy, ) chromatography, liquid chromatography–tandem mass spectrometry (Di Rago et al, ; Dukova et al, ; Flardh & Jacobson, ; Sørensen & Hasselstrøm, ), HPLC with UV–vis and fluorescent detection (Cao & Li, ; Harrison, Tonkin, & Mclean, ), HPLC‐fluorescence detection after derivatization (Tosunoglu & Ersoy, ; Wuis, Dirks, Vree, & van der Kleyn, ) and HPLC‐electrochemical detection (Millerioux, Brault, Gualano, & Mignot, ). In addition, separation of naphthalene‐2,3‐dicarboxaldehyde‐derivatves of ( RS )‐Bac has been obtained (Chang & Yang, ; Chang, Zheng, & Chen, ; Chiang, Chang, & Whang, , ) using capillary electrophoresis (CE), and a chiral capillary column was employed to separate N ‐heptafluorobutyryl isobutyl ester derivatives of ( RS )‐Bac in human plasma and urine (Sioufi, Kaiser, Leroux, & Dubois, ).…”

Section: Introductionmentioning

confidence: 99%

Enantioresolution of (RS)‐baclofen by liquid chromatography: A review

Batra

Bhushan

2016

Biomedical Chromatography

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Baclofen is a commonly used racemic drug and has a simple chemical structure in terms of the presence of only one stereogenic center. Since the desirable pharmacological effect is in only one enantiomer, several possibilities exist for the other enantiomer for evaluation of the disposition of the racemic mixture of the drug. This calls for the development of enantioselective analytical methodology. This review summarizes and evaluates different methods of enantioseparation of (RS)-baclofen using both direct and indirect approaches, application of certain chiral reagents and chiral stationary phases (though very expensive). Methods of separation of diastereomers of (RS)-baclofen prepared with different chiral derivatizing reagents (under microwave irradiation at ease and in less time) on reversed-phase achiral columns or via a ligand exchange approach providing high-sensitivity detection by the relatively less expensive methods of TLC and HPLC are discussed. The methods may be helpful for determination of enantiomers in biological samples and in pharmaceutical formulations for control of enantiomeric purity and can be practiced both in analytical laboratories and industry for routine analysis and R&D activities.

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Liquid chromatography high resolution mass spectrometry for the determination of baclofen and its metabolites in plasma: Application to therapeutic drug monitoring

Labat

Goncalves

Marques

et al. 2017

Biomedical Chromatography

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Baclofen is used to manage alcohol dependence. This study describes a simple method using liquid chromatography coupled to high-resolution mass spectrometry (LC-HR-MS) developed in plasma samples. This method was optimized to allow quantification of baclofen and determination of metabolic ratio of its metabolites, an oxidative deaminated metabolite of baclofen (M1) and its glucuronide form (M2). The LC-HR-MS method on Exactive® apparatus is a newly developed method with all the advantages of high resolution in full-scan mode for the quantification of baclofen and detection of its metabolites in plasma. The present assay provides a protein precipitation method starting with 100 μL plasma giving a wide polynomial dynamic range (R > 0.999) between 10 and 2000 ng/mL and a lower limit of quantitation of 3 ng/mL for baclofen. Intra- and inter-day precisions were <8.1% and accuracies were between 91.2 and 103.3% for baclofen. No matrix effect was observed. The assay was successfully applied to 36 patients following baclofen administration. Plasma concentrations of baclofen were determined between 12.2 and 1399.9 ng/mL and metabolic ratios were estimated between 0.4 and 81.8% for M1 metabolite and on the order of 0.3% for M2 in two samples.

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Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cited by 11 publications

References 17 publications

Untitled

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Enantioresolution of (RS)‐baclofen by liquid chromatography: A review

Liquid chromatography high resolution mass spectrometry for the determination of baclofen and its metabolites in plasma: Application to therapeutic drug monitoring

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