Rapid and sensitive detection of quizalofop-p-ethyl by gold nanoparticle-based lateral flow immunoassay in agriproducts and environmental samples

Li, Xue; Li, Jiao; Huang, Yue; Zhang, Zhongrong; Qi, Lin; Xia, Peng-Liang; Kong, Fanzuo; Qiu, Jun; Fang, Song; Hua, Xiude

doi:10.1016/j.scitotenv.2022.159427

Cited by 14 publications

(5 citation statements)

References 28 publications

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“…The concentrations of mAbs and antigens are important for the sensitivity of the CLFIA [29,30]. In our study, AuNP-mAb (1 to 6 µL) and coating antigen (0.075 to 1.2 mg mL −1 ) were used to detect pyridaben standard (0 to 80 ng mL −1 ) in PBS, with 0.1 mg mL −1 goat anti-mouse IgG fixed on the C-line.…”

Section: Clfia Optimizationmentioning

confidence: 99%

Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment

Chen

Líu

et al. 2023

Biosensors

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Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC50) of 3.49 ng mL−1. The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL−1. The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples.

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Section: Clfia Optimizationmentioning

confidence: 99%

Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment

Chen

Líu

et al. 2023

Biosensors

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“…The results indicated a detection limit of 0.16 mg/kg in banana. However, the long reaction time, tedious operating procedures, and the need for specialized equipment have limited the application of ELISAs in field testing ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive colloidal gold semiquantitative method for the determination of difenoconazole residues in citrus

Wang,

Gong,

Liu

et al. 2024

Front. Nutr.

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IntroductionDifenoconazole (DIFE) is a common pesticide used in citrus cultivation; excessive intake can cause neurological damage to the organism, and the existing colloidal gold immunochromatographic test strips cannot meet the requirements for the detection of citrus samples.MethodsDifenoconazole test strip was prepared based on the colloidal gold immunochromatographic technique (GICT), and its application in citrus samples was investigated; with colloidal gold (CG) as the probe, the optimization of GICT parameters, and the determination of reaction method, the immunochromatographic test strips for the detection of DIFE in citrus was developed, and the limit of detection (LOD), specificity, accuracy, and stability of the test strips were verified.ResultsThe results showed that the visual detection limit of the prepared colloidal gold immunochromatographic test strips was 0.2 mg/kg and the quantitative range was 0.06–0.6 mg/kg, and the test strips could specifically identify DIFE and have no cross-reaction with other common triazole pesticides. The detection method established in this study was verified by the GC–MS method, and the detection results achieved good consistency (R2 > 0.98).ConclusionThe test strips developed in this study have good performance and can be used for highly sensitive detection of citrus samples.

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“…To date, indirect competitive ELISAs (ic-ELISAs) on the basis of anti-CAP monoclonal antibody (mAb) for CAP detection in environmental and agricultural samples have shown half-inhibitory concentrations (IC 50 ) of 1.6 ng/mL [18] and 1.5 ng/mL [19]. However, the long reaction time, tedious operating procedures, and the need for specialized equipment have limited the application of ELISAs in field testing [20]. Compared with ELISAs, gold nanoparticle-based lateral flow immunoassays (AuNP-LFIAs) possess the advantages of one-step detection and do not require specialized equipment and direct visual readout [17].…”

Section: Introductionmentioning

confidence: 99%

“…Compared with ELISAs, gold nanoparticle-based lateral flow immunoassays (AuNP-LFIAs) possess the advantages of one-step detection and do not require specialized equipment and direct visual readout [17]. Therefore, they have tremendous potential for on-site rapid testing and have been used to detect various small-molecule pesticides, such as quizalofop-p-ethyl [20], quinclorac [15], and deoxynivalenol [21]. However, no AuNP-LFIA-based monoclonal antibody has been reported for CAP.…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

Wu,

Li,

Zhu

et al. 2024

Foods

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Chlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that produced anti-CAP monoclonal antibodies (mAbs), of which the mAb originating from the cell line 5C5B9 showed the highest sensitivity and was used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for CAP. The visible limit of detection of the AuNP-LFIA was 1.25 ng/mL, and the detection results were obtained in less than 10 min. The AuNP-LFIA showed no cross-reactivity for CAP analogs, except for tetraniliprole (50%) and cyclaniliprole (5%). In the detection of spiked and blind samples, the accuracy and reliability of the AuNP-LFIA were confirmed by a comparison with spiked concentrations and verified by ultra-performance liquid chromatography–tandem mass spectrometry. Thus, this study provides the core reagents for establishing CAP immunoassays and a AuNP-LFIA for the detection of residual CAP.

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Rapid and sensitive detection of quizalofop-p-ethyl by gold nanoparticle-based lateral flow immunoassay in agriproducts and environmental samples

Cited by 14 publications

References 28 publications

Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment

Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment

Development of a highly sensitive colloidal gold semiquantitative method for the determination of difenoconazole residues in citrus

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

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