Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute

A novel high sensitive, specificity duplex enzyme-activated differentiating probes PCR method for the SNP detection and differentiation of MS-H vaccine strains from wild-type Mycoplasma synoviae strains

“…The systematic evaluation showed that our method has good specificity and repeatability (all CV including inter CV and intra CV of MS-H and wild-type MS strains were lower than 5%). The method also has good sensitivity in both, which was 100 times better than that of general MS qPCR method ( Zhao et al, 2024 ). The increased sensitivity enables the detection and differentiation of even low levels of relevant pathogens at an earlier stage.…”

Pyrococcus furiosus Argonaute Based Detection Assays for Porcine Deltacoronavirus

“…Recombinase-aided amplification (RAA) technology and reverse transcription recombinase-aided amplification (RT-RAA) are to obtain recombinase from bacteria or fungi, and realize DNA or RNA amplification in vitro by cooperating with other enzymes. , In this work, RAA and RT-RAA were employed to amplify the nucleic acid before attempting PfAgo testing, since nucleic acid amplification is crucial for sensitivity in nucleic acid testing. Although RAA-PfAgo assay methods have been previously established for detecting virus, − in this study, placing the RT-RAA reaction and PfAgo in the same tube undoubtedly reduced the procedure steps as well as aerosol contamination. In this article, the terms RAA-PfAgo, RT-RAA-PfAgo, and RT-RAA-PfAgo-LFD were used to refer to these three PDCoV detection methods.…”