Rapid and sensitive detection of Staphylococcus aureus using biolayer interferometry technology combined with phage lysin LysGH15

Liu, Xiao; Huang, Chunzheng; Qiu, Cao; Wang, Zijing; Cheng, Mengjun; Zhang, Yan; Qiao, Yinghan; Yuan, Guohui; Feng, Xin; Sun, Changjiang; Li, Na; Li, Fengyang; Lei, Liancheng; Wang, Han; Zhang, Xiaoguang; Gu, Jingmin

doi:10.1016/j.bios.2021.113799

Cited by 23 publications

(10 citation statements)

References 43 publications

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“…30 Based on this principle, BLI-based aptasensors have been developed and applied for the trace detection of small molecule compounds and proteins in real samples. Liu et al 31 utilized LysGH15 as a recognition element to develop a BLI sensor for the swift identification of Staphylococcus aureus , while Gao et al 32 established a BLI-ELASA platform for the detection of glaucoma biomarkers, specifically GDF15, exhibiting favorable characteristics, such as the ease of use, reusability, and rapid detection. Hence, to facilitate the rapid and efficient monitoring of β-Lg at low concentrations, a novel aptasensor utilizing the BLI platform was developed for β-Lg quantification (Fig.…”

Section: Resultsmentioning

confidence: 99%

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

Liu,

Jiang,

et al. 2024

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

Liu,

Jiang,

et al. 2024

Anal. Methods

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“…For example, BLI assays have been developed to detect analytes in blood, light soy sauce, and even milk 38 – 42 . In addition, antibodies or phage components, such as receptor binding proteins or lysins, have been used to functionalize BLI biosensors for detecting foodborne pathogens 40 , 41 . Biosensors functionalized with antibodies specific for Salmonella enterica can detect contamination as low as 1.6 × 10 5 colony-forming units per milliliter (CFU/mL) in less than 300 seconds 41 .…”

Section: Discussionmentioning

confidence: 99%

Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform

Needham,

Page,

Yehl

2024

Sci Rep

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The continuing and rapid emergence of antibiotic resistance (AMR) calls for innovations in antimicrobial therapies. A promising, ‘re-emerging’ approach is the application of bacteriophage viruses to selectively infect and kill pathogenic bacteria, referred to as phage therapy. In practice, phage therapy is personalized and requires companion diagnostics to identify efficacious phages, which are then formulated into a therapeutic cocktail. The predominant means for phage screening involves optical-based assays, but these methods cannot be carried out in complex media, such as colored solutions, inhomogeneous mixtures, or high-viscosity samples, which are often conditions encountered in vivo. Moreover, these assays cannot distinguish phage binding and lysis parameters, which are important for standardizing phage cocktail formulation. To address these challenges, we developed Phage-layer Interferometry (PLI) as a companion diagnostic. Herein, PLI is assessed as a quantitative phage screening method and prototyped as a bacterial detection platform. Importantly, PLI is amenable to automation and is functional in complex, opaque media, such as baby formula. Due to these newfound capabilities, we foresee immediate and broad impact of PLI for combating AMR and protecting against foodborne illnesses.

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“…Researchers have also explored utilizing bacteriophage-derived affinity molecules as recognition elements rather than the entire bacteriophage structures. Bacteriophage lysin (LysGH15) and long tail fibers have been recently incorporated as recognition elements to detect S. aureus and Salmonella spp., respectively ( Liu et al, 2022 ; Wang et al, 2022a ). LysGH15 lost its lytic activity through C54A mutation but retained its recognizing and binding ability to S. aureus .…”

Section: Bacteriophage-based Biosensorsmentioning

confidence: 99%

“…LysGH15 lost its lytic activity through C54A mutation but retained its recognizing and binding ability to S. aureus . Liu et al (2022) combined biolayer interferometry (BLI) with LysGH15, acting as its bioreceptor. The BLI-based method was able to detect S. aureus whole cells directly in food samples (ice cube and light soy sauce) with a LOD of 13 CFU/ml with a binding time of 12 min.…”

Section: Bacteriophage-based Biosensorsmentioning

confidence: 99%

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

et al. 2022

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Traditional foodborne pathogen detection methods are highly dependent on pre-treatment of samples and selective microbiological plating to reliably screen target microorganisms. Inherent limitations of conventional methods include longer turnaround time and high costs, use of bulky equipment, and the need for trained staff in centralized laboratory settings. Researchers have developed stable, reliable, sensitive, and selective, rapid foodborne pathogens detection assays to work around these limitations. Recent advances in rapid diagnostic technologies have shifted to on-site testing, which offers flexibility and ease-of-use, a significant improvement from traditional methods’ rigid and cumbersome steps. This comprehensive review aims to thoroughly discuss the recent advances, applications, and limitations of portable and rapid biosensors for routinely encountered foodborne pathogens. It discusses the major differences between biosensing systems based on the molecular interactions of target analytes and biorecognition agents. Though detection limits and costs still need further improvement, reviewed technologies have high potential to assist the food industry in the on-site detection of biological hazards such as foodborne pathogens and toxins to maintain safe and healthy foods. Finally, this review offers targeted recommendations for future development and commercialization of diagnostic technologies specifically for emerging and re-emerging foodborne pathogens.

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Rapid and sensitive detection of Staphylococcus aureus using biolayer interferometry technology combined with phage lysin LysGH15

Cited by 23 publications

References 43 publications

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

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