Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Giesendorf, B. A. J.; Quint, Wim; Henkens, M. H. C.; Stegeman, H.; Huf, Fernanda; Niesters, H. G. M.

doi:10.1128/aem.58.12.3804-3808.1992

Cited by 144 publications

(62 citation statements)

References 23 publications

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“…Several DNA-based methods have been developed for the detection and identification of Campylobacter species in food, clinical and environmental samples (Oyofo et al 1992;Oyofo and Rollins 1993;Eyers et al 1993;Uyttendaele et al 1994;Korolik et al 1995;Stucki et al 1995;Cardarelli-Leite et al 1996;Jackson et al 1996;Giesendorf et al 1992Giesendorf et al , 1993. Although these methods are faster than conventional methods, the efficacy is often limited, since they require multiple PCR reactions per sample or digestions with multiple restriction enzymes.…”

Section: Discussionmentioning

confidence: 99%

Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Doorn¹,

Haperen²,

Belkum³

et al. 1998

J Appl Microbiol

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Campylobacters isolated from mussels and oysters in The Netherlands were analysed by a novel assay, based on DNA amplification with primers, based on semiconserved GTP‐binding sites of a putative GTPase gene. Polymerase chain reaction (PCR) was followed by a single step reverse hybridization line probe assay (PCR‐LiPA). This permits identification of Campylobacter jejuni, C. coli, C. lari and C. upsaliensis. Among a group of 44 isolates, three C. jejuni, one C. upsaliensis, one double infection with C. jejuni and C. coli, and 38 C. lari strains were identified. These results were in complete agreement with conventional identification methods and whole cell protein analysis. One C. hyointestinalis isolate was not identified by the PCR‐LiPA, since the reverse hybridization assay does not comprise specific probes for this particular species. PCR products from 36 C. lari isolates were sequenced and phylogenetic analysis revealed the presence of two major C. lari subgroups: one comprised 11 highly homologous sequences, whereas the 25 sequences in the other subgroup were more heterogeneous. This confirmed earlier findings that C. lari isolates comprise a more heterogeneous group of isolates as compared with C. jejuni, C. coli and C. upsaliensis. Based on the sequence information, a novel PCR‐LiPA was developed that permits specific and rapid detection of the different C. lari variants.

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Section: Discussionmentioning

confidence: 99%

Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Doorn¹,

Haperen²,

Belkum³

et al. 1998

J Appl Microbiol

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“…DNA probes and an oligonucleotide primer pair which do not hybridize to DNA from other Campylobacter species, especially the closely related thermophilic C. coli, C. lari and C. upsaliensis, were selected. Other workers have already developed PCR tests for the detection of C. jejuni, but C. coli was also detected [15,16]. In a recent study [17], the PCR fingerprinting technique was successfully applied to the specific detection of C. jejuni.…”

Section: Discussionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) techniques were developed for the detection of C. jejuni and C. coli. The oligonucleotide primers used in the PCR tests were chosen from a DNA fragment coding for the C. coli flagellin [15] or from a 16S ribosomal RNA gene [16]. Nevertheless, none of these methods can be used for the specific identification of C. jejuni, since cross-reactions with 338 other thermophilic Campylobacter species were observed.…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuni: Specific oligonucleotides and DNA probes for use in polymerase chain reaction-based diagnosis

Stonnet

1993

FEMS Immunology and Medical Microbiology

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A 1189 base-pair long DNA fragment, VS1, was isolated from a Campylobacter jejuni CIP 70.2 cosmid library and was found to contain regions specific for this bacterial species. For detection and identification of C. jejuni, two oligonucleotides derived from the VS1 sequence were used as primers in polymerase chain reaction tests on genomic DNAs from 38 campylobacter and from 10 non-Campylobacter strains. A specific, 358 base-pair long DNA fragment was amplified only when C. jejuni DNA was used as a target. The detection limit of the amplification reaction was as low as 1.86 fg DNA, which is the equivalent of one C. jejuni genome.

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“…Simple and dependable protocols are currently being developed for the application of PCR detection of several pathogenic food microorganisms in a range of matrices: e.g. Listeria in milk [35], cheese [33,36] and meat products [37]; Salmonella and Campylobacter in poultry ( [24,38], respectively).…”

Section: Rapid Detectionmentioning

confidence: 99%

Microbes in food processing technology

Hofstra

Vossen

Plas

1994

FEMS Microbiology Reviews

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There is an increasing understanding that the microbial quality of a certain food is the result of a chain of events. It is clear that the microbial safety of food can only be guaranteed when the overall processing, including the production of raw materials, distribution and handling by the consumer are taken into consideration. Therefore, the microbiological quality assurance of foods is not only a matter of control, but also of a careful design of the total process chain. Food industry has now generally adapted quality assurance systems and is implementing the Hazard Analysis Critical Control Point (HACCP) concept. Rapid microbiological monitoring systems should be used in these cases. There is a need for rapid and simple microbiological tests which can be adapted to the technology and logistics of specific production processes. Traditional microbiological methods generally do not meet these high requirements. This paper discusses the tests, based on molecular biological principles, to detect and identify microbes in food-processing chains. Tests based on DNA technology are discussed, including in vitro DNA amplification like the polymerase chain reaction (PCR) method and identifications based on RFLP, RAPD and DNA fingerprinting analysis. PCR-based methodology can be used for the rapid detection of microbes in food manufacturing environments. In addition, DNA fingerprinting methods are suitable for investigating sources and routes of microbial contamination in the food cycle.

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Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Cited by 144 publications

References 23 publications

Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Campylobacter jejuni: Specific oligonucleotides and DNA probes for use in polymerase chain reaction-based diagnosis

Microbes in food processing technology

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