Rapid and sensitive detection of SARS-CoV-2 virus in human saliva samples using glycan based nanozyme: a clinical study

Rokni, Mehrdad; Rohani Bastami, Tahereh; Meshkat, Zahra; Reza Rahimi, Hamid; Zibaee, Saeed; Meshkat, Mojtaba; Fotouhi, Fatemeh; Serki, Elham; Khoshakhlagh, Mahdieh; Dabirifar, Zeynab

doi:10.1007/s00604-023-06120-3

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Cited by 3 publications

References 49 publications

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Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis

Zhan,

Wang,

Yang

et al. 2024

Environment International

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Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis

Zhan,

Wang,

Yang

et al. 2024

Environment International

View full text Add to dashboard Cite

Nanozyme-based therapeutic strategies for rheumatoid arthritis

Zhang,

Wang,

et al. 2025

Journal of Controlled Release

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Extraction-free colorimetric RT-LAMP for SARS-CoV-2 RNA detection from saliva

Yang,

Liu,

Tie

et al. 2024

Preprint

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AimTo develop a rapid and easy diagnostic assay for detection of SARS-CoV-2 RNA presented in saliva samples.MethodThe color-based RT-LAMP was used to detect nucleocapsid (N) gene of SARS-CoV-2 without RNA extraction from saliva.ResultsRNA spiked saliva can be used directly for cDNA synthesis after heat inactivation of the saliva and diluted 2-fold with either water or PBS. For both PCR and LAMP, 20% of saliva did not have obvious effect on the reaction. Saliva did not interfere with RT-LAMP when the volume of saliva was less than 20% of the total volume. The sensitivity of the RT-LAMP reached to 1.034×10-5ng/µl (475 copies/μl). The RT-LAMP assay was validated by testing 20 RNA spiked saliva samples. The assay specificity was similar to that of data without saliva.ConclusionsThe RT-LAMP colorimetric assay can be used as a screening method with the advantages of being rapid, easy to use than the qRT-PCR.

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Rapid and sensitive detection of SARS-CoV-2 virus in human saliva samples using glycan based nanozyme: a clinical study

Cited by 3 publications

References 49 publications

Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis

Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis

Nanozyme-based therapeutic strategies for rheumatoid arthritis

Extraction-free colorimetric RT-LAMP for SARS-CoV-2 RNA detection from saliva

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