Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis

Cheng, Hao; Lai, Long‐Li; Ko, Fu‐Hsiang

doi:10.2147/ijn.s32240

Cited by 14 publications

(7 citation statements)

References 18 publications

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“…Human VEGF ELISA kit was used to measure the amount of VEGF protein in accordance with the manufacturer's instruction. 48 A microplate reader was used to record the optical density (OD) values at 450 nm. All experiments were repeated three times.…”

Section: Elisamentioning

confidence: 99%

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Sun¹,

Wang²,

Cui³

et al. 2018

IJN

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BackgroundGraphene oxide (GO) has attracted intensive interest in biological and medical fields in recent years due to its unique physical, chemical, and biological properties. In our previous work, we proved that GO could deliver small interfering RNA (siRNA) into cells and downregulate the expression of the desired gene.MethodsThis study investigated the potential of a modified GO nanocarrier for co-delivery of siRNA and doxorubicin (DOX) for enhanced cancer therapy. Fourier transform infrared spectroscopy, laser particle size analyzer, UV-visible spectroscopy, gel electrophoresis retardation, and in vitro release assay were studied.ResultsThe results of real-time polymerase chain reaction revealed that the expression of vascular endothelial growth factor (VEGF) mRNA was decreased 46.84%±3.72% (mean ± SD). Enzyme-linked immunosorbent assay indicated that the expression of VEGF protein was down-regulated to 52.86%±1.10% (mean ± SD) in vitro. In vivo tumor growth assay GO-poly-l-lysine hydrobromide/folic acid (GPF)/DOX/siRNA exhibited gene silencing and tumor inhibition (66.95%±2.35%, mean ± SD) compared with naked siRNA (1.62%±1.47%, mean ± SD) and DOX (33.63%±5.85%, mean ± SD). GPF/DOX/siRNA exhibited no testable cytotoxicity.ConclusionThe results indicated that co-delivery of siRNA and DOX by GPF could be a promising application in tumor clinical therapy.

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Section: Elisamentioning

confidence: 99%

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Sun¹,

Wang²,

Cui³

et al. 2018

IJN

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“…1 These features render them the great potential for various applications like magnetic storage devices, 2 ferrofluids, 3 magnetic separation/biomolecular purification, 4 MRI contrast agents, targeted drug delivery, 5 hyperthermia, 6 immunoassay, 7 biosensing, 8 tissue regeneration, 9 cell sorting, 10 molecular interaction in live cells 11 and catalysis. 12 For most of the biomedical applications, it is desirable to make them water soluble containing biocompatible components.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of glucose-mediated Ag–γ-Fe₂O₃multifunctional nanocomposites in aqueous medium – a kinetic analysis of their catalytic activity for 4-nitrophenol reduction

2015

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“…Such a process has been shown to be a simple and highly efficient method to enrich specific antigens from a mixed antigen population (27)(28)(29). Magnetic beads coated with antibodies recognize and bind to the target antigens.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool

Huang

Jin

Zhang³

et al. 2014

J Clin Microbiol

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We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarrheal stool specimens, 53 (13.2%) were toxin-producing C. difficile strains by quantitative toxigenic culture (qTC); bacterial loads ranged from 3.00 ؋ 10 1 to 3.69 ؋ 10 6 CFU/ml. The RTCA version 2 method detected CDT in 51 samples, resulting in a sensitivity of 96.2%, a specificity of 99. C lostridium difficile infection (CDI) is the leading bacterial cause of nosocomial diarrhea in hospitalized patients (1-4). The incidence of CDI has increased in the last decade and community onset disease has been encountered in patients without previous health care exposure or antibiotic use (5-7).A definitive laboratory diagnosis of CDI depends on a stool test result positive for the presence of toxigenic C. difficile or its toxins (CDT) or colonoscopic or histopathologic findings demonstrating pseudomembranous colitis (8). Currently, laboratory methods used for the diagnosis of CDI are based on detection of C. difficile-specific antigen (e.g., glutamate dehydrogenase enzyme immunoassay [EIA]), toxin proteins (toxin A/B EIA, toxigenic culture [TC], cell cytotoxicity neutralization assay [CCNA]), or toxin genes (real-time PCR [RT-PCR] and loop-mediated isothermal amplification assay) (9-14). Molecular diagnostic techniques, including RT-PCR and other molecular assays, have the advantages of higher sensitivities and rapid turnaround times and are increasingly being implemented for routine diagnostic use (15-17).While molecular-based tests have high sensitivity and are faster, these assays detect the presence of toxin-encoding genes, and they may not be able to distinguish between infection and colonization as well as live and dead C. difficile organisms in certain circumstances (18,19). Recent studies highlighted the importance of detecting and quantifying CDT (20,21). Planche et al. recently noted no increase in mortality when toxigenic C. difficile alone was present, while toxin determined by cytotoxin assay positivity correlated with clinical outcome. CDT correlated with the clinical severity of CDI (21). Leslie et al. found that toxin-negative patients had a lower level of C. difficile than toxin-positive patients (22). These results suggested that toxin quantification could be used clinically to predict toxin status and help distinguish patients with CDI from carriers with diarrhea due to other causes (21).We previously reported on the use of a real-time cellular analysis (RTCA) assay (ACEA Biosciences, San Diego, CA) for quantitative detection of CDT directly from stool (23). This assay is based on microelectronic sensor-based cellular analysis technology (23). Cell index (CI) is a dimensionless parameter to represent cell status based on the change of the electrode impedance. The impedance readout harness...

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Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis

Cited by 14 publications

References 18 publications

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Synthesis of glucose-mediated Ag–γ-Fe₂O₃multifunctional nanocomposites in aqueous medium – a kinetic analysis of their catalytic activity for 4-nitrophenol reduction

Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool

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Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis

Cited by 14 publications

References 18 publications

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Synthesis of glucose-mediated Ag–γ-Fe2O3multifunctional nanocomposites in aqueous medium – a kinetic analysis of their catalytic activity for 4-nitrophenol reduction

Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool

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Synthesis of glucose-mediated Ag–γ-Fe₂O₃multifunctional nanocomposites in aqueous medium – a kinetic analysis of their catalytic activity for 4-nitrophenol reduction