Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes

Steinmetz, Lukas; Bourquin, Joël; Barošová, Hana; Haeni, Laetitia; Caldwell, Jessica; Milošević, Ana; Geers, Christoph; Bonmarin, Mathias; Taladriz‐Blanco, Patricia; Rothen‐Rutishauser, Barbara; Petri‐Fink, Alke

doi:10.1039/d0nr03330h

Cited by 5 publications

(6 citation statements)

References 52 publications

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“…It is noted that increased uptake of nanotubes by macrophages could be due to their phagocytic activity and may cause fibrosis, which is also confirmed in the toxicogenomic analysis of macrophages treated with similar CNTs concentrations and exposure time [68]. Besides, a 3D rendering approach was applied to assess the internalisation of "long and thick" Mitsui-7 and "short and thin" Nanocyl-7000 MWCNTs on various cell types (bronchial and alveolar epithelial cells, mouse macrophages, and mesothelial cells) [69] as well as a complex organotypic model of alveolar tissue [70]. Nanocyl-7000 nanotubes appeared as densely packed aggregates in both cases, while Mitsui-7 were distinguishable as individual nanotubes.…”

Section: In Vitro Studies Of Cntsmentioning

confidence: 83%

Dark-Field Hyperspectral Microscopy for Carbon Nanotubes Bioimaging

Ishmukhametov

Fakhrullin

2021

Applied Sciences

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Carbon nanotubes have emerged as a versatile and ubiquitous nanomaterial, finding applications in industry and biomedicine. As a result, biosafety concerns that stimulated the research focused on evaluation of carbon nanotube toxicity. In addition, biomedical applications of carbon nanotubes require their imaging and identification in biological specimens. Among other methods, dark-field microscopy has become a potent tool to visualise and identify carbon nanotubes in cells, tissues, and organisms. Based on the Tyndall effect, dark-field optical microscopy at higher magnification is capable of imaging nanoscale particles in live objects. If reinforced with spectral identification, this technology can be utilised for chemical identification and mapping of carbon nanotubes. In this article we overview the recent advances in dark-field/hyperspectral microscopy for the bioimaging of carbon nanotubes.

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Section: In Vitro Studies Of Cntsmentioning

confidence: 83%

Dark-Field Hyperspectral Microscopy for Carbon Nanotubes Bioimaging

Ishmukhametov

Fakhrullin

2021

Applied Sciences

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“…In previous studies we demonstrated that spatial variations can account for up to 18 % in signal variations [9][10][11][12][13][14]. Trigger input/output lines are connected to the infrared camera, whereas USB connection to the computer enables the selection of wavelength and intensity.…”

Section: Non-homogeneous Stimulationmentioning

confidence: 99%

“…To localize water ingress for example, microwaves are particularly suited [6,7], whereas eddy currents will be more appropriate to detect cracks in metallic materials [8]. We demonstrated that active thermal imaging is a promising tool for engineered NPs [9][10][11][12][13][14]. Lock-in thermal imaging (LIT) records the sample surface temperature during the application of a harmonic heating stimulus at a defined frequency [15].…”

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confidence: 99%

Using Lock-In Thermography to Investigate Stimuli-Responsive Nanoparticles in Complex Environments

Bonmarin

Steinmetz

Spano

et al. 2021

IEEE Instrum. Meas. Mag.

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The use of nanoparticles (NP) has been dramatically rising in the recent years and NPs can nowadays be found in various products ranging from food to composite materials or cosmetics [1,2]. Currently, the most frequently employed NP types are titania (TiO2), as a typical color additive, silica (SiO2), as anticoagulation agent, and silver (Ag) NPs, which are added to textiles, due to their antimicrobial properties. Because of their outstanding physical and mechanical properties carbon-containing nanomaterials, such as graphene and carbon nanotubes, have also experienced a surge in industrially relevant applications. About 30 % of the NPs are suspended in liquids, ranging from water to creams and lotions to car lubricants, followed by applications containing surface-bound (e.g., in textiles) NPs and finally nanocomposites (e.g., polymer-CNT composites). Together with biological and physiological fluids, these matrices render the detection and quantification of NPs fairly complex [3]. The broad range of chemical and biological compositions of these environments, including pH and ionic strength are often detrimental to the colloidal stability of NPs, potentially causing aggregation or even dissolution effects and therefore render NP analysis fairly challenging.Man-made, so-called 'engineered' NPs can be precisely tailored with respect to specific and desired properties. These materials are often stimuli-responsive meaning that they demonstrate the ability to generate heat upon a stimulus: alternating magnetic fields (AMF) for magnetic particles like superparamagnetic iron oxide NPs (SPIONs), and light in the UV, visible or NIR range for plasmonic NPs like gold spheres and other photothermal particles. Such a behavior

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“…13 With LIT, NPs can be investigated in situ, in a contactless and non-intrusive manner even in complex environments, such as in a cell culture medium-containing serum. 20 As temperature changes during LIT measurements do not exceed the millikelvin range, phase transformations and NP melting or reshaping can be excluded. Furthermore, fluorescent labeling and staining of NPs is redundant, which is especially beneficial for biological studies.…”

Section: Introductionmentioning

confidence: 99%

“…The advantage of using AuNPs for such a study lies in their well-known dielectric functions, high stability, uniformity, and their resonance wavelengths being in the visible range . With LIT, NPs can be investigated in situ , in a contactless and non-intrusive manner even in complex environments, such as in a cell culture medium-containing serum . As temperature changes during LIT measurements do not exceed the millikelvin range, phase transformations and NP melting or reshaping can be excluded.…”

Section: Introductionmentioning

confidence: 99%

Experimental and Theoretical Validation of Plasmonic Nanoparticle Heat Generation by Using Lock-In Thermography

et al. 2021

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The use of plasmonic nanoparticles for biological applications has been gaining momentum in the last two decades. The ability of these particles to generate heat when exposed to light with a given wavelength is one of their prominent features. Quantifying the heat emission and relating it to the shape, size, and concentration of particles is of great importance. In this work, we show how lock-in thermography, a technique where temperature changes are obtained by means of an infrared camera, can be used to measure efficiently and non-invasively the heat generated by plasmonic nanoparticle solutions exposed to a modulated light source. We developed a mathematical model based on energy balance, where the heat generated by particles is computed from the absorption cross section of particles using Mie theory. The model, free of adjustable parameters, can quantitatively predict the heat generated by gold nanoparticles in suspensions in a broad range of concentrations and for two different wavelengths, which were experimentally investigated. The model and experimental data show how the amplitude of the temperature response increases linearly with the gold concentration, and is almost independent of the investigated particle size.

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Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes

Abstract: For the first time lock-in thermography is utilized to assess cell-associated nanoparticles and carbon nanotubes.

Cited by 5 publications

References 52 publications

Dark-Field Hyperspectral Microscopy for Carbon Nanotubes Bioimaging

Dark-Field Hyperspectral Microscopy for Carbon Nanotubes Bioimaging

Using Lock-In Thermography to Investigate Stimuli-Responsive Nanoparticles in Complex Environments

Experimental and Theoretical Validation of Plasmonic Nanoparticle Heat Generation by Using Lock-In Thermography

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