2011
DOI: 10.1556/amicr.58.2011.1.7
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Rapid and simple approach for identification of Mycobacterium tuberculosis and M. bovis by detection of regulatory gene whiB7

Abstract: Identification of Mycobacterium tuberculosis and M. bovis is necessary for the application of adequate drug therapy. PCR amplification is a good tool for this purpose, but choosing proper target is of a great concern. We describe a PCR assay for fast detection of M. tuberculosis and M. bovis.As a BLAST and BLASTP search we selected regulatory gene whiB7 that encodes multi-drug resistance in this bacterium. Thirty clinical isolates of M. tuberculosis were sequenced and all the mutations in gene whiB7 were detec… Show more

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Cited by 9 publications
(6 citation statements)
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“…Samples were diluted to 20% (w/v) in PBS (pH 7.4). DNA was isolated using NewGene Preamp (Simbios Biotecnologia, Cachoeirinha, RS, Brazil) based on guanidine isothiocyanate and silica [16]. RNA was isolated using TRIzol ® Reagent (Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions.…”
Section: Sample Collection Nucleic Acids Isolation and Cdna Synthesismentioning
confidence: 99%
“…Samples were diluted to 20% (w/v) in PBS (pH 7.4). DNA was isolated using NewGene Preamp (Simbios Biotecnologia, Cachoeirinha, RS, Brazil) based on guanidine isothiocyanate and silica [16]. RNA was isolated using TRIzol ® Reagent (Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions.…”
Section: Sample Collection Nucleic Acids Isolation and Cdna Synthesismentioning
confidence: 99%
“…To date, 23 types of CPV were reported by the Papillomavirus Episteme (PaVE) database (https://pave.niaid.nih.gov) allocated into three distinct genera. CPV1 and 6 are classified as Lambdapapillomavirus: CPV2, 7,13,17,19,21,22, and 23 as Taupapillomavirus, and the Chipapillomavirus genus is composed by the remaining types (CPV3, 4, 5, 8, 9, 10, 11, 12, 14, 15, 16 18, and 20) [6]. The genus Chipapillomavirus is divided in three species: Chipapillomavirus 1, composed by CPV3, 5, 9, 11, and 12; Chipapillomavirus 2, composed by CPV4 and 16; and Chipapillomavirus 3, composed by the types CPV8, 10, 14, and 15.…”
Section: Introductionmentioning
confidence: 99%
“…Recent studies have characterized deregulation of host miRNAs following infection with extracellular ( H. pylori ) (12), or intracellular ( Salmonella enterica ) (13), Gram-negative bacteria, as well as in the response to Gram-positive ( Listeria monocytogenes ) (14) and other pathogenic bacteria ( Mycobacterium (15) and Francisella species (16)).…”
Section: Introductionmentioning
confidence: 99%