Rapid and simple detection of Japanese encephalitis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick

Deng, Jieru; Pei, Jingjing; Gou, Hongchao; Ye, Zuodong; Liu, Cuicui; Chen, Jinding

doi:10.1016/j.jviromet.2014.12.006

Cited by 37 publications

(15 citation statements)

References 23 publications

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“…The RT-PCR tests, quantitative PCR (TaqMan), restriction fragment length polymorphism (RFLP) analysis are useful molecular assay tests as they are very specific, sensitive and can detect low viral copies in acute or early phase of infection [ 45 ]. An RT-LAMP-LFD assay that combines reverse transcription loop-mediated isothermal amplification (RT-LAMP) with a lateral flow dipstick (LFD), is of great importance in diagnosis of JEV infection as it is a fast, highly sensitive and specific assay [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Japanese Encephalitis: A Brief Review on Indian Perspectives

Kulkarni

Sapkal

Kaushal

et al. 2018

TOVJ

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Introduction:Japanese encephalitis (JE) is recently declared as a notifiable disease in India due to its expanding geographical distribution. The disease notification facilitates effective implementation of preventive measures and case management. Expalantion:JE is a vector-borne disease that can be prevented by vaccine administration. It is caused by Japanese encephalitis virus (JEV), belonging to family Flaviviridae. Amongst the known etiological viral encephalitis agents, it is one of the leading viral agents of acute encephalitis syndrome in many Asian countries where it is identified to cause substantial morbidity and mortality as well as disability. Globally, it is responsible for approximately 68,000 clinical cases every year.Conclusion:In the absence of antivirals, patients are given supportive treatment to relieve and stabilize. Amongst available control strategies; vector control is resource intensive while animal and human vaccination are the most effective tool against the disease. This review highlights recent progress focusing challenges with diagnosis and prophylactic interventions.

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Section: Introductionmentioning

confidence: 99%

Japanese Encephalitis: A Brief Review on Indian Perspectives

Kulkarni

Sapkal

Kaushal

et al. 2018

TOVJ

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“…Worldwide in 2014, it is estimated that there were 9.6 million people infected with TB and 1.5 million deaths due to TB[15]. Of these deaths, more than one quarter were HIV-infected patients, a population where current diagnostics often fail [3,16].…”

Section: Discussionmentioning

confidence: 99%

Design and use of mouse control DNA for DNA biomarker extraction and PCR detection from urine: Application for transrenal Mycobacterium tuberculosis DNA detection

Bordelon

Ricks

Pask

et al. 2017

Journal of Microbiological Methods

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Urine samples are increasingly used for diagnosing infections including Escherichia coli, Ebola virus, and Zika virus. However, extraction and concentration of nucleic acid biomarkers from urine is necessary for many molecular detection strategies such as polymerase chain reaction (PCR). Since urine samples typically have large volumes with dilute biomarker concentrations making them prone to false negatives, another impediment for urine-based diagnostics is the establishment of appropriate controls particularly to rule out false negatives. In this study, a mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) DNA target was added to retrospectively collected urine samples from tuberculosis (TB)-infected and TB-uninfected patients to indicate extraction of intact DNA and removal of PCR inhibitors from urine samples. We tested this design on surrogate urine samples, retrospective 1 milliliter (mL) urine samples from patients in Lima, Peru and retrospective 5 mL urine samples from patients in Cape Town, South Africa. Extraction/PCR control DNA was detectable in 97% of clinical samples with no statistically significant differences among groups. Despite the inclusion of this control, there was no difference in the amount of TB IS6110 Tr-DNA detected between TB-infected and TB-uninfected groups except for samples from known HIV-infected patients. We found a increase in TB IS6110 Tr-DNA between TB/HIV co-infected patients compared to TB-uninfected/HIV-infected patients (N=18, p=0.037). The inclusion of an extraction/PCR control DNA to indicate successful DNA extraction and removal of PCR inhibitors should be easily adaptable as a sample preparation control for other acellular sample types.

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“…Moreover, some conventional molecular biology methods, such as RT-PCR, nested RT-PCR and real-time PCR, have inherent flaws; in particular, specialized PCR instruments are needed, and temperature must be precisely controlled for a lengthy period of time. This inhibits the application of these methods in rural and remote areas ( Deng et al, 2015 ). As the reaction conditions for RT-LAMP-VF are isothermal, only a water bath is needed to complete the assay, offering a measure of practicality for field laboratories in economically impoverished areas.…”

Section: Discussionmentioning

confidence: 99%

Visual Detection of West Nile Virus Using Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Vertical Flow Visualization Strip

Cao

Wang

et al. 2016

Front. Microbiol.

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West Nile virus (WNV) causes a severe zoonosis, which can lead to a large number of casualties and considerable economic losses. A rapid and accurate identification method for WNV for use in field laboratories is urgently needed. Here, a method utilizing reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip (RT-LAMP-VF) was developed to detect the envelope (E) gene of WNV. The RT-LAMP-VF assay could detect 102 copies/μl of an WNV RNA standard using a 40 min amplification reaction followed by a 2 min incubation of the amplification product on the visualization strip, and no cross-reaction with other closely related members of the Flavivirus genus was observed. The assay was further evaluated using cells and mouse brain tissues infected with a recombinant rabies virus expressing the E protein of WNV. The assay produced sensitivities of 101.5 TCID50/ml and 101.33 TCID50/ml for detection of the recombinant virus in the cells and brain tissues, respectively. Overall, the RT-LAMP-VF assay developed in this study is rapid, simple and effective, and it is therefore suitable for clinical application in the field.

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Rapid and simple detection of Japanese encephalitis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick

Cited by 37 publications

References 23 publications

Japanese Encephalitis: A Brief Review on Indian Perspectives

Japanese Encephalitis: A Brief Review on Indian Perspectives

Design and use of mouse control DNA for DNA biomarker extraction and PCR detection from urine: Application for transrenal Mycobacterium tuberculosis DNA detection

Visual Detection of West Nile Virus Using Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Vertical Flow Visualization Strip

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