Rapid and simple technique for the determination of the refractive index of ultra-thin organic films on planar transparent substrates using forward light scatter

Henke, Lisa; Piunno, Paul A. E.; Nagy, Noémi M.; Wust, Christopher C.; Krull, Ulrich J.

doi:10.1016/s0003-2670(00)01285-x

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…For the DNA submonolayers used in this study, probe and target coverages were determined by fixing the dielectric constant of the layer (ɛ layer ) at a value of 2.0 ( 19 ). Angularly dependent light scattering and ellipsometry studies have reported differences in ɛ layer of 0.06–0.2 for ssDNA and dsDNA films ( 23 , 24 ). Our assumption of a single value of ɛ layer for all probes with varying degrees of secondary structure, therefore, can cause at most 3% error in our coverage calculations.…”

Section: Methodsmentioning

confidence: 99%

Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison

Gao

2006

Nucleic Acids Research

302

318

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The hybridization kinetics for a series of designed 25mer probe–target pairs having varying degrees of secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution and on the surface, respectively. Kinetic rate constants derived from the resultant data decrease with increasing probe and target secondary structure similarly in both solution and surface environments. Specifically, addition of three intramolecular base pairs in the probe and target structure slow hybridization by a factor of two. For individual strands containing four or more intramolecular base pairs, hybridization cannot be described by a traditional two-state model in solution-phase nor on the surface. Surface hybridization rates are also 20- to 40-fold slower than solution-phase rates for identical sequences and conditions. These quantitative findings may have implications for the design of better biosensors, particularly those using probes with deliberate secondary structure.

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Section: Methodsmentioning

confidence: 99%

Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison

Gao

2006

Nucleic Acids Research

302

318

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“…An extensive body of literature related to the fabrication and characterization of OTCE is currently available. However, most of these studies have focused on either the optical (mostly in the visible region of the electromagnetic spectrum) or electrochemical properties of OTCE.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and electrochemical characterization of nanostructured optically transparent carbon electrodes

Benavidez

Garcı́a

2013

Electrophoresis

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The present paper describes the results related to the optical and electrochemical characterization of thin carbon films fabricated by spin coating and pyrolysis of AZ P4330-RS photoresist. The goal of this paper is to provide comprehensive information allowing for the rational the selection of the conditions to fabricate optically-transparent carbon electrodes (OTCE) with specific electro-optical properties. According to our results, these electrodes could be appropriate choices as electrochemical transducers to monitor electrophoretic separations. At the core of this manuscript is the development and critical evaluation of a new optical model to calculate the thickness of the OTCE by variable angle spectroscopic ellipsometry (VASE). Such data was complemented with topography and roughness (obtained by AFM), electrochemical properties (obtained by cyclic voltammetry), electrical properties (obtained by electrochemical impedance spectroscopy), and structural composition (obtained by Raman spectroscopy). Although the described OTCE were used as substrates to investigate the effect of electrode potential on the real-time adsorption of proteins by ellipsometry, these results could enable the development of other biosensors that can be then integrated into various CE platforms.

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Fluorimetric DNA Biosensors

Piunno

Krull

Topics in Fluorescence Spectroscopy

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Rapid and simple technique for the determination of the refractive index of ultra-thin organic films on planar transparent substrates using forward light scatter

Cited by 8 publications

References 17 publications

Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison

Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison

Spectroscopic and electrochemical characterization of nanostructured optically transparent carbon electrodes

Fluorimetric DNA Biosensors

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