Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography

Amini, Hossein; Ahmadiani, Abolhassan

doi:10.1016/j.jpba.2006.09.004

Cited by 54 publications

(35 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1) was an important advance in the development of clinically effective antimicrobial agents. This combination is recommended for the treatment of infections in the upper respiratory tract and lower urinary tract, as well as for kidney, gastrointestinal, and other bacterial infections [17]. It is also widely used in prophylaxis and the treatment of veterinary infections [4,5].…”

Section: Introductionmentioning

confidence: 99%

Determination of Sulfamethoxazole and Trimethoprim and Their Metabolites in Hospital Effluent

Brenner

Mallmann

Arsand

et al. 2010

CLEAN Soil Air Water

View full text Add to dashboard Cite

In this work, an analytical methodological study was carried out to determine the antimicrobials sulfamethoxazole and trimethoprim, as well as their metabolites, in hospital effluent. The determinations were conducted by liquid chromatography tandem mass spectrometry using a hybrid triple quadrupole-linear ion trap mass spectrometer (LC-QqLIT-MS). The data acquisition was made in selected reaction monitoring (SRM) mode, in which two SRM transitions were monitored to ensure that the target compounds were accurately identified by the information dependent acquisition (IDA) function. The limits of detection (LOD) and quantification (LOQ) were 0.25 and 0.80 mg L À1 for sulfamethoxazole and 0.15 and 0.50 mg L À1 for trimethoprim. The linear range for the SMX was 0.8-100.0 mg L À1 and TMP was 0.5-100.0 mg L À1 on the basis of sixpoint calibration curves generated by means of linear regression analysis. The coefficients of the correlation were higher than 0.999, which ensured the linearity of the method. The average concentration of sulfamethoxazole and trimethoprim found in hospital effluent was 27.8 and 6.65 mg L À1 , respectively. The analytical methodology employed allowed two metabolites to be identified, N4-acetyl-sulfamethoxazole and ahydroxy-trimethoprim. Fragmentation pathways were proposed.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of Sulfamethoxazole and Trimethoprim and Their Metabolites in Hospital Effluent

Brenner

Mallmann

Arsand

et al. 2010

CLEAN Soil Air Water

View full text Add to dashboard Cite

show abstract

“…Samples were analyzed by high-performance liquid chromatography (HPLC) [11], with an Agilent 1200 series HPLC system (Agilent Technologies, Santa Clara, Calif., USA). Plasma samples (250 μl) were deproteinated with 65% perchloric acid (20 μl).…”

Section: Methodsmentioning

confidence: 99%

Trimethoprim and Sulfamethoxazole Transmembrane Clearance during Modeled Continuous Renal Replacement Therapy

Kesner¹,

Yardman-Frank²,

Mercier

et al. 2014

Blood Purif

View full text Add to dashboard Cite

Background/Aims: There is limited data regarding trimethoprim (TMP)/sulfamethoxazole (SMX) continuous renal replacement therapy (CRRT) dosing. We aimed to estimate TMP/SMX transmembrane clearance (CLtm) during continuous hemofiltration (CH) and continuous hemodialysis (CD) to guide dosing. Methods: Using an in vitro model, TMP/SMX sieving coefficients (SC) and saturation coefficients (SA) were determined with high-flux polyarylethersulfone and polyacrylonitrile-sodium methallyl sulfonate copolymer hemodiafilters at ultrafiltration/dialysate rates of 1, 2, 3, and 6 l/h. TMP/SMX CLtm was calculated using measured SC and SA. TMP/SMX CRRT doses were modeled using CLtm and published TMP/SMX pharmacokinetic parameters. Results: TMP SC/SA during CH/CD were significantly higher than SMX SC/SA. During modeling, TMP 10 mg/kg/day and its corresponding SMX dose, 50 mg/kg/day, resulted in steady state TMP/SMX peak concentrations associated with efficacy against Pneumocystis jirovecii. Conclusions: CRRT resulted in greater TMP CLtm than SMX. TMP 10 mg/kg/day divided q12h may be an appropriate initial dose to consider in patients undergoing CRRT.

show abstract

“…[18][19][20][21][22][23][24] An alternative method for determination of these sulfonamides is HPLC with an electrochemical detector (HPLC-ED) using the amperometric technique. Klimes and Mokry Rao 25 carried out analysis for four sulfonamides (sulfamethoxazole, sulfamoxazole, sulfisoxazole and sulfamethoxydiazine) by HPLC with electrochemical detector using a glassy carbon electrode.…”

Section: Introductionmentioning

confidence: 99%

Determination of sulfamethoxazole in pharmaceutical formulations by flow injection system/HPLC with potentiometric detection using polypyrrole electrode

Özkorucuklu¹,

Şahin²,

Alsancak³

2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Neste trabalho, um eletrodo de polipirrol (PPy) foi utilizado como detector eletroquímico potenciométrico em um sistema de injeção em fluxo para a determinação de sulfametoxazol em formulações farmacêuticas. O eletrodo PPy foi preparado por voltametria cíclica em solução de acetonitrila. Uma relação linear foi observada no intervalo de concentração de 2,5 × 10 -5 a 1,25 × 10 -3 mol L -1 com um coeficiente de correlação de 0,9977 e limite de detecção de 1,03 × 10 -6 mol L -1(S/N = 3). As recuperações em cápsulas e xaropes foram 97,4 e 90,8% com desvios padrões relativos de 0,62 e 1,04%, respectivamente, concordando com valores obtidos por cromatografia líquida de alto rendimento (HPLC) com detector de UV. Então, concluiu-se que o eletrodo PPy pode ser usado como material detector potenciométrico alternativo para a determinação de sulfametoxazol em formulações farmacêuticas com as vantagens de fácil preparação e capacidade de regeneração da superfície do eletrodo.In this work, it was used a polypyrrole (PPy) electrode as a potentiometric electrochemical detector in a flow injection system in order to determine sulfamethoxazole in pharmaceutical formulations. The PPy electrode was prepared by cyclic voltammetry in acetonitrile solution. A linear relationship was observed over the concentration range of 2.5 × 10 -5 -1.25 × 10 -3 mol L -1 with a correlation coefficient of 0.9977 and limit of detection (LOD) of 1.03 × 10 -6 mol L -1 (S/N = 3). The recoveries in tablet and syrup formulations were found as 97.4 and 90.8% with the relative standard deviations of 0.62 and 1.04%, respectively, which closely agree with those measured by high performance liquid chromatography (HPLC) with UV detector. Therefore, it was concluded that the PPy electrode can be used as an alternative novel potentiometric detector material for determination of sulfamethoxazole in pharmaceuticals with the advantages of easy preparation and regeneration capability of the electrode surface.

show abstract

Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography

Cited by 54 publications

References 16 publications

Determination of Sulfamethoxazole and Trimethoprim and Their Metabolites in Hospital Effluent

Determination of Sulfamethoxazole and Trimethoprim and Their Metabolites in Hospital Effluent

Trimethoprim and Sulfamethoxazole Transmembrane Clearance during Modeled Continuous Renal Replacement Therapy

Determination of sulfamethoxazole in pharmaceutical formulations by flow injection system/HPLC with potentiometric detection using polypyrrole electrode

Contact Info

Product

Resources

About