Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices

Acuña, Inmaculada; Ruiz, Alberto Matarán; Cerdó, Tomás; Cantarero, Samuel; López‐Moreno, Ana; Aguilera, Margarita; Campoy, Cristina; Suárez, Antonio

doi:10.1002/biof.1766

Cited by 18 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histidine, by the action of the enzyme histidine decarboxylase, is converted into histamine, a potent mediator of numerous physiological reactions. Histamine is considered a neurotransmitter with different functions in various disorders of the central nervous system, including insomnia, Parkinson’s disease, schizophrenia, Alzheimer’s disease and cerebral ischemia [ 73 , 74 ].…”

Section: Resultsmentioning

confidence: 99%

An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

et al. 2023

View full text Add to dashboard Cite

Pesticides constitute a category of chemical products intended specifically for the control and mitigation of pests. With their constant increase in use, the risk to human health and the environment has increased proportionally due to occupational and environmental exposure to these compounds. The use of these chemicals is associated with several toxic effects related to acute and chronic toxicity, such as infertility, hormonal disorders and cancer. The present work aimed to study the metabolic profile of individuals occupationally exposed to pesticides, using a metabolomics tool to identify potential new biomarkers. Metabolomics analysis was carried out on plasma and urine samples from individuals exposed and non-exposed occupationally, using liquid chromatography coupled with mass spectrometry (UPLC-MS). Non-targeted metabolomics analysis, using principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) or partial least squares discriminant orthogonal analysis (OPLS-DA), demonstrated good separation of the samples and identified 21 discriminating metabolites in plasma and 17 in urine. The analysis of the ROC curve indicated the compounds with the greatest potential for biomarkers. Comprehensive analysis of the metabolic pathways influenced by exposure to pesticides revealed alterations, mainly in lipid and amino acid metabolism. This study indicates that the use of metabolomics provides important information about complex biological responses.

show abstract

Section: Resultsmentioning

confidence: 99%

An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Metabolites that decreased in the human feces and this murine model include N-acetylhistamine and ethylmalonate. N-acetylhistamine is a gut microbial-derived metabolite of histidine metabolism and can act as a stimulant of gastric secretion [ 73 ]. High levels of ethylmalonate observed in the rice bran group mice may indicate improved fatty acid transport associated with rice bran.…”

Section: Discussionmentioning

confidence: 99%

Integrated Microbiota and Metabolite Changes following Rice Bran Intake during Murine Inflammatory Colitis-Associated Colon Cancer and in Colorectal Cancer Survivors

Weber

Ibrahim

Baxter

et al. 2023

Cancers

View full text Add to dashboard Cite

Dietary rice bran-mediated inhibition of colon carcinogenesis was demonstrated previously for carcinogen-induced rodent models via multiple anti-cancer mechanisms. This study investigated the role of dietary rice bran-mediated changes to fecal microbiota and metabolites over the time course of colon carcinogenesis and compared murine fecal metabolites to human stool metabolic profiles following rice bran consumption by colorectal cancer survivors (NCT01929122). Forty adult male BALB/c mice were subjected to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated colon carcinogenesis and randomized to control AIN93M (n = 20) or diets containing 10% w/w heat-stabilized rice bran (n = 20). Feces were serially collected for 16S rRNA amplicon sequencing and non-targeted metabolomics. Fecal microbiota richness and diversity was increased in mice and humans with dietary rice bran treatment. Key drivers of differential bacterial abundances from rice bran intake in mice included Akkermansia, Lactococcus, Lachnospiraceae, and Eubacterium xylanophilum. Murine fecal metabolomics revealed 592 biochemical identities with notable changes to fatty acids, phenolics, and vitamins. Monoacylglycerols, dihydroferulate, 2-hydroxyhippurate (salicylurate), ferulic acid 4-sulfate, and vitamin B6 and E isomers significantly differed between rice bran- and control-fed mice. The kinetics of murine metabolic changes by the host and gut microbiome following rice bran consumption complemented changes observed in humans for apigenin, N-acetylhistamine, and ethylmalonate in feces. Increased enterolactone abundance is a novel diet-driven microbial metabolite fecal biomarker following rice bran consumption in mice and humans from this study. Dietary rice bran bioactivity via gut microbiome metabolism in mice and humans contributes to protection against colorectal cancer. The findings from this study provide compelling support for rice bran in clinical and public health guidelines for colorectal cancer prevention and control.

show abstract

“…Enrichment of such cellular and genetic processing pathways suggests active cellular activity in Bengali populations. On the other hand, histidine is essential for muscle carnosine synthesis, and its metabolism is associated with physical endurance, recognition memory, neural protection, satiety, and allergic diseases 101,102 . The presence of this metabolic pathway in the Chakma and Marma tribes’ samples indicates that SCFAs play an essential role in the metabolism of their diet ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiome Composition Reveals the Distinctiveness between the Bengali people and the Indigenous Ethnicities in Bangladesh

Ahammad¹,

Rahman²,

Chowdhury³

et al. 2023

Preprint

View full text Add to dashboard Cite

Human gut microbiome is influenced by ethnicity and other factors. In this study, we have explored the gut microbiome of Bengali population (n=13) and four Tibeto-Burman indigenous communities- Chakma (n=15), Marma (n=6), Khyang (n=10), and Tripura (n=11) using 16S rRNA amplicon sequencing. A total of 19 characterized phyla were identified in 5 cohorts, with Firmicutes and Bacteroidetes being the most prevalent. At the genus level, the abundance of Prevotella was relatively similar across all ethnicities. However, the Chakma population demonstrated higher Bacteroides abundance. Chakma people were more distinct than other ethnicities and exhibited a higher quantity of differentially abundant microbial features. The Bengali population had relatively low bacterial richness and Firmicutes to Bacteroidetes ratio than others with lower qualitative microbial diversity. A phylosymbiotic link between Bangladeshi indigenous people and certain ethnic groups in India have also been discovered. A comparative analysis between all Bangladeshi samples (n=55) and several tropical and subtropical countries (n=132) such as Australia, Egypt, Indonesia, Malaysia, Mexico, Thailand, and Vietnam revealed that the gut microbiota profile of Bangladeshi people is remarkably distinct from others. The insights from this study will aid further epidemiological and translational research.

show abstract

Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices

Cited by 18 publications

References 49 publications

An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

Integrated Microbiota and Metabolite Changes following Rice Bran Intake during Murine Inflammatory Colitis-Associated Colon Cancer and in Colorectal Cancer Survivors

Gut Microbiome Composition Reveals the Distinctiveness between the Bengali people and the Indigenous Ethnicities in Bangladesh

Contact Info

Product

Resources

About