2021
DOI: 10.1021/jacs.1c04925
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Rapid and Specific Imaging of Extracellular Signaling Molecule Adenosine Triphosphate with a Self-Phosphorylating DNAzyme

Abstract: Adenosine 5′-triphosphate (ATP) is a central extracellular signaling agent involved in various physiological and pathological processes. However, precise measurements of the temporal and spatial components of ATP dynamics are lacking due primarily to the limitations of available methods for ATP detection. Here, we report on the first effort to design a self-phosphorylating DNAzyme (SPDz) sensor for fluorescence imaging of ATP. In response to ATP, SPDz sensors exhibit subsecond response kinetics, extremely high… Show more

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Cited by 45 publications
(39 citation statements)
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“…[174] Tracking the localization and changes in extracellular ATP facilitates our understanding of relevant cellular processes, although its accurate measurement remains a great challenge. [175] To pursue this goal, Yuan et al employed a DNA tweezer and split aptamer to develop a ratio metric DNA nanoswitch that can be anchored on cell surfaces based on hydrophobic insertion. [175b] The structure of the nanos witch can be altered upon binding two split aptamers with ATP, thus inducing a change in FRET efficiency.…”
Section: Engineering Cell Surfaces For Biosensingmentioning
confidence: 99%
“…[174] Tracking the localization and changes in extracellular ATP facilitates our understanding of relevant cellular processes, although its accurate measurement remains a great challenge. [175] To pursue this goal, Yuan et al employed a DNA tweezer and split aptamer to develop a ratio metric DNA nanoswitch that can be anchored on cell surfaces based on hydrophobic insertion. [175b] The structure of the nanos witch can be altered upon binding two split aptamers with ATP, thus inducing a change in FRET efficiency.…”
Section: Engineering Cell Surfaces For Biosensingmentioning
confidence: 99%
“…To addressing this challenge, Zhao et al designed a cellsurface-anchored self-phosphorylating DNAzyme (SPDz) sensor (Figure 2c). 129 In the presence of ATP, the substrate strand enables transfer of a phosphate group from ATP to its 5′ end, achieving self-phosphorylation. In this case, the substrate strands can be digested by DNAzyme, thus generating fluorescence signal.…”
Section: Applications Of Nucleic Acid-based Cell Surface Engineeringmentioning
confidence: 99%
“…Only in the presence of both H + and K + ions, the DNA device can be activated for the sensing of ATP on target cell membranes. Instead of using aptamers, the Li group demonstrated that the cell membrane detection of ATP can also be achieved based on DNAzymes (Zhao et al ., 2021). Upon binding with ATP, the DNAzyme can catalyze a nucleophile attacking of its own 5’-hydroxyl group on the ATP phosphate, which results in a self-phosphorylation reaction and a λ -exonuclease-based cleavage of the DNAzyme strand to generate an activated fluorescence signal.…”
Section: Lipid-dna Conjugates For Detecting Localized Membrane Signalingmentioning
confidence: 99%