Rapid and ultra-sensitive determination of enzyme activities using surface-enhanced resonance Raman scattering

Moore, Barry D.; Stevenson, Lorna; Watt, Alan P.; Flitsch, Sabine L.; Turner, Nicholas J.; Cassidy, Chris; Graham, Duncan

doi:10.1038/nbt1003

Cited by 182 publications

(125 citation statements)

References 29 publications

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“…Thus SERRS has been used to study heme proteins, 9 to study the mechanism of electron transfer in cytochromes, 10 and more recently for trace detection of DNA 11,12 and enzyme activity. 13 The sensitivity of SERRS exceeds that of fluorescence, which is currently the most prolific technique employed for studying biomolecules, as proven theoretically 14 and experimentally. 15 SER(R)S has another immense advantage over fluorescence in that the signals have extremely small line-widths compared to the broad spectra of fluorescence emission; and hence it is possible, using SER(R)S labels, to carry out multiplexed detection.…”

Section: Introductionmentioning

confidence: 99%

Reproducible SERRS from structured gold surfaces

Mahajan

Baumberg

Russell

et al. 2007

Phys. Chem. Chem. Phys.

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Metallic substrates with ordered spherical cavities have been shown to be very effective for surface-enhanced Raman scattering (SERS) and can be fabricated reproducibly using electrodeposition. The sensitivity of detection is increased by several orders of magnitude by using surface-enhanced resonance Raman scattering (SERRS). In this report we demonstrate SERRS for the first time on electrodeposited gold films templated with colloidal spheres and demonstrate the reproducibility of the response. We also obtain a direct comparison between SERRS and SERS by choosing two dyes, Cy5t and Cy3t, which are similar in structure but differ in their excitation maxima, such that one is resonant and the other non-resonant with our laser excitation. As expected, the resonant enhancement is found to be of the order of 10 3 over and above that for SERS. The net SERRS enhancements are shown to be of the order of 10 9 . We also find that the resonant enhancement profile of the different peaks for the chromophore follows the plasmonic resonance absorption spectrum obtained for the structured surface.

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Section: Introductionmentioning

confidence: 99%

Reproducible SERRS from structured gold surfaces

Mahajan

Baumberg

Russell

et al. 2007

Phys. Chem. Chem. Phys.

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“…Recently, a type of SERS-active substrate with uniformly large and highly reproducible Raman-enhancing power has been developed by growing Ag nanoparticles on arrays of anodic aluminum oxide (AAO) nanochannels to take advantage of the sub-10-nm inter-particle gaps, which act as 'hot junctions' for creating the electromagnetic enhancement 4 . The high sensitivity and reproducibility of such a substrate-hereafter referred to as Ag/AAO-SERS substrate-facilitated the use of SERS for chemical/biological sensing applications [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] . SERS of various types of bacteria including Gram-positive, Gram-negative and mycobacteria have been acquired and the response of bacteria to antibiotics has been examined 21 .…”

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confidence: 99%

Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

et al. 2011

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Detecting bacteria in clinical samples without using time-consuming culture processes would allow rapid diagnoses. such a culture-free detection method requires the capture and analysis of bacteria from a body fluid, which are usually of complicated composition. Here we show that coating Ag-nanoparticle arrays with vancomycin (Van) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (sERs), leading to a ~1,000-fold increase in bacteria capture, without introducing significant spectral interference. Bacteria from human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, a Van-coated substrate provides distinctly different sERs spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance tests. our results represent a critical step towards the creation of sERs-based multifunctional biochips for rapid culture-and label-free detection and drug-resistant testing of microorganisms in clinical samples.

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“…[76] SERS can be used to enhance native Raman signals, by close proximity to roughened metal surfaces. However, perhaps the more promising application is to probe for specific interactions, for example by detection of specific antigens [37] or enzyme activity [18,77]. Kneipp et al[78] demonstrated the use of SERS to enhance Raman signals in single cells, therefore decreasing the acquisition time for a single map significantly.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

“…[37,[80][81][82] SERRS based assays have also been used to detect enzyme activity. Moore et al [77] designed an assay to detect a variety of enzyme activities including from lipases, esterases and proteases. In these assays, a substrate consisting of a recognition site for a particular enzyme, a linker that can be cleaved by the particular enzyme and a SERRS-active dye was synthesised.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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. (2017). Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? Vibrational Spectroscopy, vol. 91, July, pp. 16-30. doi.org/10.1016/ j.vibspec.2016 Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? AbstractVibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction of drugs with cells. Many studies have demonstrated the ability to detect biochemical changes in cells in response to drug application, using both infrared and Raman spectroscopy. This has shown potential for use in high throughput screening (HTS) applications, for screening of efficacy and mode of action of potential drug candidates, to speed up the drug discovery process. HTS is still a relatively new and growing area of research and, therefore, there is more potential for new techniques to move into and shape this field. Vibrational spectroscopic techniques come with many benefits over the techniques used currently in HTS, primarily based on fluorescence assays to detect specific binding interactions or phenotypes. They are label free, and an infrared or Raman spectrum provides a wealth of biochemical information, and therefore could reveal not only information about a specific interaction, but about how the overall biochemistry of a cell changes in response to application of a drug candidate. Therefore, drug mode of action could be elucidated. This review will investigate the potential for vibrational spectroscopy, particularly FTIR and Raman spectroscopy, to benefit the field of HTS and improve the drug development process. In addition to FTIR and Raman spectroscopy, surface enhanced Raman spectroscopy (SERS), coherent anti-Stokes Raman spectroscopy (CARS) and stimulated Raman spectroscopy (SRS), will be investigated as an alternative tool in the HTS process.

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Rapid and ultra-sensitive determination of enzyme activities using surface-enhanced resonance Raman scattering

Cited by 182 publications

References 29 publications

Reproducible SERRS from structured gold surfaces

Reproducible SERRS from structured gold surfaces

Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

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