Rapid and ultrasensitive detection of DNA and microRNA-21 using a zirconium porphyrin metal-organic framework-based switch fluorescence biosensor

Quasi-intrinsic fluorescent probes for detecting the DNA adduct (ABPdG) based on an excited-state intermolecular charge transfer mechanism

“…7 In 2022, a switch fluorescence biosensor was developed to sense DNA and microRNA-21 via the adsorption–quenching–recovery mechanism. 8…”

“…7 In 2022, a switch fluorescence biosensor was developed to sense DNA and microRNA-21 via the adsorption-quenching-recovery mechanism. 8 Despite the high sensitivity and easy accessibility of fluorescence detection, the used extrinsic fluorophores tend to interfere with the native structure and associated biological functions of DNA. 9 Accordingly, there is a crucial need to develop a more sophisticated fluorescence labelling strategy to report changes in the site-specific conformation and microenvironment with minimal interference with the native structure.…”

Rapid and Ultrasensitive Fluorescence Sensing Platform Based on Nanometer-Sized Metal–Organic Frameworks for Transgenic CaMV 35S Promoter Detection

“…Therefore, nucleic acid probes are sharply adsorbed on Fe-MIL-88 through π−π stacking, electrostatic interaction, and hydrogen bond. 24,48 The addition of the target makes FAM-ssDNA more inclined to form dsDNA with CaMV 35S, resulting in the nucleic acid bases of the probe buried in the phosphoric acid skeleton, and thus detached from the surface of Fe-MIL-88. 49 The fluorescence quenching mechanism of Fe-MIL-88 on the FAM group is further investigated.…”

“…The interaction between fluorescent probes and MOFs mainly depends on π–π interaction, hydrogen bonding, and electrostatic forces . The internal fluorescence quenching mechanisms are ascribed to PET and FRET . However, once the target is specifically bound to ssDNA to form dsDNA, ssDNA will be detached from the material surface and fluorescence is restored .…”

Platform Formed from ZIF-8 and DNAzyme: “Turn-On” Fluorescence Assay for Simple, High-Sensitivity, and High-Selectivity Detection of Pb2+

“…Metal–organic frameworks (MOFs), a class of crystalline hybrid materials, are obtained with the combination of metal ions or metal cluster nodes and organic ligands . In recent years, several MOFs have been utilized as quenchers toward dye-labeled DNA, such as MIL-101, ZrMOF, UiO-66-NH 2 , and ZIF-8 . Compared with conventional 1D/2D materials, such as gold nanoparticles (AuNPs), graphene oxide (GO), molybdenum disulfide (MoS 2 ), and tungsten disulfide (WS 2 ), three-dimensional (3D) MOFs have large surface areas, diverse structures, and unique physical and chemical properties .…”