2024
DOI: 10.3390/ijms25042430
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Rapid Antibacterial Activity Assessment of Chimeric Lysins

Jin-Mi Park,
Jun-Hyun Kim,
Gun Kim
et al.

Abstract: Various chimeric lysins have been developed as efficacious antibiotics against multidrug-resistant bacteria, but direct comparisons of their antibacterial activities have been difficult due to the preparation of multiple recombinant chimeric lysins. Previously, we reported an Escherichia coli cell-free expression method to better screen chimeric lysins against Staphylococcus aureus, but we still needed to increase the amounts of expressed proteins enough to be able to detect them non-isotopically for quantity … Show more

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