Rapid aptasensor capable of simply diagnosing prostate cancer

Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7

“…4, we modified EA-5 to enhance the strength of light emitted in guanine chemiluminescence reaction as EA-6 (5′-6-FAM-GGGGGGTTTTCCGGACGCTTATGCCTTGCCATCTACAG-AGCGGTGTGACGG-3′) based on research result reported by Cha and his collaborators [12]. 6-FAM conjugated with 5′ end of aptamer was directly connected 6 guanines without changing the sequence of E. Coli O157:H7 aptamer.…”

“…Guanine, one of the four components -Adenine, Cytosine, Guanine, Thymine -of DNA aptamer, when reacting with 3,4,5-trimethoxylphenylglyoxal hydrate (TMPG), can produce a highenergy intermediate capable of emitting light by itself or transferring energy to fluorescent dye-conjugated with DNA aptamer based on the principle of intra chemiluminescence resonance energy transfer (Intra-CRET) as shown in Scheme 1 [11][12][13]. Light emitted from the reaction of DNA aptamer and TMPG in the presence of tetra-n-propylammonium hydroxide (TPA) and N,Ndimethylformamide (DMF) is called guanine chemiluminescence [11].…”

“…Light emitted from the reaction of DNA aptamer and TMPG in the presence of tetra-n-propylammonium hydroxide (TPA) and N,Ndimethylformamide (DMF) is called guanine chemiluminescence [11]. Using two distinguishing characteristics (e.g., capture capable of rapidly binding with target material, CL reagent in guanine chemiluminescence reaction) of DNA aptamer, an aptasensor capable of rapidly sensing thrombin or prostate specific antigen (PSA) without time-consuming incubations and washings was developed [11,12].…”

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

“…Liu et al (2012) performed Differential Pulse Voltammetry (DPV) measurements on graphite electrode with gold nanoparticles encapsulated by graphitized mesoporous carbon, exploiting biotinylated-aptamer/streptavidin coupling, achieving a limit of detection (LOD) of 7 pM with a dynamic range from 7 pM to 6 nM. Cha et al (2014) reported on a chemiluminescent resonance energy transfer aptasensor for PSA with a LOD of 30 pM. A chemiluminescent aptasensor able to remove impurities that hamper PSA detection in human serum with sensitivity comparable to enzyme immunoassays was developed by Choi & Lee (2013).…”

Biomarkers towards Ovarian Cancer Diagnostics: Present and Future Prospects

“…Due to the different properties of G-rich DNA aptamer-conjugated 6-FAM in the absence and presence of PSA in guanine chemiluminescence reaction, even trace levels of PSA in human serum can be quantified within 30 min without time-consuming and complicated procedures. The limit of detection (LOD) of this biosensor having a wide linear dynamic range (1.9-125 ng/ml) was 1.0 ng/ml 99 . Similar findings clearly demonstrate that aptamer-based approaches can also potentially target ovarian cancer.…”