Rapid Assessment of the Bacteriological Quality of Raw Milk Using ATP Bioluminescence

Samkutty, Pushpa J.; Gough, R. H.; Adkinson, R.W.; McGrew, P.

doi:10.4315/0362-028x-64.2.208

Cited by 34 publications

(9 citation statements)

References 6 publications

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“…Microbial ATP bioluminescence assays have been applied for rapid assessment of bacterial contamination on the surfaces of meat carcasses (38), poultry carcasses (13), whole cantaloupes (40), in drinking water (12), and milk (34).…”

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confidence: 99%

Microbiological Safety of Domestic Refrigerators and the Dishcloths Used To Clean Them in Guadalajara, Jalisco, Mexico

Macías-Rodríguez¹,

Navarro-Hidalgo²,

Linares-Morales³

et al. 2013

Journal of Food Protection

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Household refrigerators are a potential pathogen contamination source for foods. An evaluation of the microbiological safety of 200 refrigerators in Guadalajara, Jalisco, Mexico, was made by visual inspection, ATP-bioluminescence levels, indicator microorganisms including Escherichia coli and Staphylococcus aureus, and the presence of Listeria monocytogenes and Salmonella. Additionally, interviews of the owners of the refrigerators were carried out to determine relationships between food storage practices, demographic aspects, and microbiological status. Dishcloths used to clean refrigerators were also analyzed. Operational conditions (cleanliness, fullness, organization, frequency of cleaning, and temperature) were evaluated by trained observers. Results showed deficient cleanliness in 55% of refrigerators, 22% were completely full, 43% very disorganized, 28% were usually cleaned only once in 3 to 6 months, and 53% had internal temperatures >7.1°C. ATP-bioluminescence levels were >300 relative light units on 67 and 74% of shelves and drawers, respectively, indicating that surfaces were dirty according to the luminometer manufacturer. Psychrotrophic aerobic bacteria counts on shelves, drawers, and dishcloths were 6.3, 5.2, and 6.3 log CFU/cm(2); for coliform bacteria, 5.2, 3.9, and 4.7 CFU/cm(2); for E. coli, 3.7, 3.5, and 4.8 CFU/cm(2); and for Staphylococcus aureus, 2.1, 2.5, and 2.3 CFU/cm(2), respectively. L. monocytogenes and Salmonella were isolated from 59.5, 20.5, and 17% and 32.5, 8.0 and 12.5% of shelves, drawers, and dishcloths, respectively. Four Salmonella serotypes and nine serogroups (partially serotyped isolates) were identified. The most prevalent were Salmonella Anatum (39.5%), Salmonella group E1 (19.7%), and Salmonella group E1 monophasic (12.5%). Operational conditions and microbiological status were clearly deficient in sampled refrigerators, highlighting the consequent risk of foodborne disease among users. Educational programs are needed to improve the domestic food safety in Mexico.

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confidence: 99%

Microbiological Safety of Domestic Refrigerators and the Dishcloths Used To Clean Them in Guadalajara, Jalisco, Mexico

Macías-Rodríguez¹,

Navarro-Hidalgo²,

Linares-Morales³

et al. 2013

Journal of Food Protection

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“…Modifications of microbial AP bioluminescence procedures which require from 5 to 15 min and estimate total microbial contamination in foods have been reported (Griffiths 1991(Griffiths , 1993Bautista et al 1995;Siragusa et al 1995;Samkutty et al 2001). However, the values of ATP are variable in foods and a specific instrument is needed for the ATP analysis (Siragusa et al 1995).…”

Section: Discussionmentioning

confidence: 97%

Use of a simple catalase assay for assessment of aerobic microbial contamination on vegetables

2010

Ann Microbiol

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This paper presents a rapid catalase test for monitoring the aerobic microbial contamination associated with vegetables. The microbial loads of celery, bell pepper and ready-to-eat salad were serially tested over a 2-week period under common storage conditions. At each time point, samples were surface-sampled for catalase activity with a Pasteur pipette method in 5 min. Simultaneously, the aerobic viable microbial counts of the samples were determined using PCA, PIA and DRBC plates. It was found that the catalase activity (% gas produced) in the sample is positively correlated to the log concentration of aerobic bacteria and fungi. The catalase activity assay appears to be a fairly effective method for rapid assessment of aerobic microbial contamination on vegetables tested. The model based on the correlation between catalase activity and aerobic microbial loads was established and tested, with results that show accuracy of prediction at higher levels of contamination.

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“…Bioluminescence lends itself well to rapid spot monitoring, as the results are obtained in less than 15 min (Feng 2001). This technique has been used on a diverse array of foods, including raw and pasteurized dairy products (Webster et al 1988;Kyriakides 1992;Brovko et al 1999;Niza-Ribeiro et al 2000;Samkutty et al 2001), meat and poultry products (Bautista et al 1994(Bautista et al , 1995Siragusa and Cutter 1995;Chen 2000), beer (Dowhanick and Sobczak 1994), and fruit (Ukuku et al 2001).…”

Section: Atp Bioluminescencementioning

confidence: 99%

A review of conventional detection and enumeration methods for pathogenic bacteria in food

Gracias¹,

McKillip²

2004

Can. J. Microbiol.

333

193

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With continued development of novel molecular-based technologies for rapid, high-throughput detection of foodborne pathogenic bacteria, the future of conventional microbiological methods such as viable cell enumeration, selective isolation of bacteria on commercial media, and immunoassays seems tenuous. In fact, a number of unique approaches and variations on existing techniques are currently on the market or are being implemented that offer ease of use, reliability, and low cost compared with molecular tools. Approaches that enhance recovery of sublethally injured bacteria, differentiation among species using fluorogenics or chromogenics, dry plate culturing, differentiation among bacteria of interest using biochemical profiling, enumeration using impedence technology, techniques to confirm the presence of target pathogens using immunological methods, and bioluminescence applications for hygiene monitoring are summarized here and discussed in relation to their specific advantages or disadvantages when implemented in a food microbiology setting.

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Rapid Assessment of the Bacteriological Quality of Raw Milk Using ATP Bioluminescence

Cited by 34 publications

References 6 publications

Microbiological Safety of Domestic Refrigerators and the Dishcloths Used To Clean Them in Guadalajara, Jalisco, Mexico

Microbiological Safety of Domestic Refrigerators and the Dishcloths Used To Clean Them in Guadalajara, Jalisco, Mexico

Use of a simple catalase assay for assessment of aerobic microbial contamination on vegetables

A review of conventional detection and enumeration methods for pathogenic bacteria in food

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