2024
DOI: 10.1186/s12936-024-04915-0
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Rapid assessment of the blood-feeding histories of wild-caught malaria mosquitoes using mid-infrared spectroscopy and machine learning

Emmanuel P. Mwanga,
Idrisa S. Mchola,
Faraja E. Makala
et al.

Abstract: Background The degree to which Anopheles mosquitoes prefer biting humans over other vertebrate hosts, i.e. the human blood index (HBI), is a crucial parameter for assessing malaria transmission risk. However, existing techniques for identifying mosquito blood meals are demanding in terms of time and effort, involve costly reagents, and are prone to inaccuracies due to factors such as cross-reactivity with other antigens or partially digested blood meals in the mosquito gut. This study demonstra… Show more

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Cited by 3 publications
(2 citation statements)
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“…The variations may be attributed to factors including ecological differences, variations in human population densities, employment of different mechanisms by humans against mosquito bites, and mosquito behaviors [12,15]. It is also might be related with the variation in the study design, sampling methods, and blood meal analysis techniques as well [39,40], making the present review challenging to draw de nitive conclusions. For instance, the lowest and highest HBI values for An.…”
Section: Discussionmentioning
confidence: 95%
“…The variations may be attributed to factors including ecological differences, variations in human population densities, employment of different mechanisms by humans against mosquito bites, and mosquito behaviors [12,15]. It is also might be related with the variation in the study design, sampling methods, and blood meal analysis techniques as well [39,40], making the present review challenging to draw de nitive conclusions. For instance, the lowest and highest HBI values for An.…”
Section: Discussionmentioning
confidence: 95%
“…To obtain reference labels of P. falciparum sporozoite infections in the mosquito head and thorax carcasses, we used real-time PCR targeting var gene acidic terminal sequences ( varATS ) of the parasites 9 , 10 and Enzyme-linked immunosorbent assays (ELISA) assays for detecting circumsporozoite protein (CSP) 8 . Each carcass underwent individual analysis, a method previously demonstrated for detecting mosquito blood meal remaining 45 . In total, 7178 An.…”
Section: Methodsmentioning
confidence: 99%