Rapid asymmetrical evolution of <i>Saccharomyces cerevisiae</i> wine yeasts

Ambrona, Jesús; Vinagre, Antonia; Ramírez, Manuel

doi:10.1002/yea.1331

Cited by 17 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Commercial and native yeast isolates display greater genomic and genetic instability than laboratory strains (Ambrona et al 2005), and aberrations in the number of some chromosomes are common (Bakalinsky and Snow 1990). Wild strains are generally homothallic and show low sporulation rates and poor spore viability.…”

Section: Functional Diversity Of Wine Strainsmentioning

confidence: 99%

Geographic Origin and Diversity of Wine Strains ofSaccharomyces

Bisson¹

2012

Am. J. Enol. Vitic.

View full text Add to dashboard Cite

Abstract:The availability of genome sequence information from a large collection of strains of Saccharomyces isolated from a variety of geographic regions and ecological niches has enabled a detailed analysis of genome composition and phenotype evolution, the two components of strain diversity. These analyses have also provided a relatively complete depiction of the origins of wine strains. In population genomic analysis, wine strains of S. cerevisiae cluster as a highly related group, but one that shows a greater level of phenotypic differentiation than would be predicted based on the level of genomic similarity. Natural and human selection and genetic drift have played roles in the evolution of wine strain diversity. Phenotypic diversity is so extensive that no one strain accurately represents all wine strains with respect to biological properties and fermentation performance. In addition, both commercial and native isolates have been found to carry introgressions, regions of DNA derived from nonhomologous organisms, suggestive of cell fusion events with yeast of different genera and species. Comparative sequence analysis has thus refined our knowledge of yeast lineages and offers explanation for the evolution of phenotypic diversity observed in winery and vineyard populations.

show abstract

Section: Functional Diversity Of Wine Strainsmentioning

confidence: 99%

Geographic Origin and Diversity of Wine Strains ofSaccharomyces

Bisson¹

2012

Am. J. Enol. Vitic.

View full text Add to dashboard Cite

show abstract

“…High genetic instability and LOH in natural wine yeasts during laboratory propagation under nonselective conditions, but not in the common laboratory strains of S. cerevisiae, have been described recently (3,14). Also, a high LOH has been reported at the URA3 locus in a transgenic wine yeast strain during must fermentation (12).…”

mentioning

confidence: 99%

“…In S. cerevisiae, genetic instability is associated with a high rate of LOH (3,9,14). High genetic instability and LOH in natural wine yeasts during laboratory propagation under nonselective conditions, but not in the common laboratory strains of S. cerevisiae, have been described recently (3,14).…”

mentioning

confidence: 99%

“…Also, the expected frequency of heterozygous yeasts at the beginning of the second must fermentation, inoculated with tetrads from a yeast population containing 15% G418 R /G418 R , 13% SMR R / SMR R , and 72% G418 R /SMR R , is 0.72 ϫ 0.7125 ϭ 0.513, which is very close to the frequency found, 0.54 (day 2, Table 3). That is, LOH in homothallic wine yeast occurs because of the linkage of the locus MAT to the chromosome III centromere, without the need for self spore clone mating (10), mitotic gene conversion (12), or rapid asymmetric LOH due to genetic instability (3,14). This phenomenon is enough in itself to explain the high level of homozygosity found in natural populations of wine yeasts (10,13).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Homothallic Saccharomyces cerevisiae Strain Mating during Must Fermentation

Ambrona

Ramírez

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Genetic instability and genome renewal may cause loss of heterozygosity (LOH) in homothallic wine yeasts (Saccharomyces cerevisiae), leading to the elimination of the recessive lethal or deleterious alleles that decrease yeast fitness. LOH was not detected in genetically stable wine yeasts during must fermentation. However, after sporulation, the heterozygosity of the new yeast population decreased during must fermentation. The frequency of mating between just-germinated haploid cells from different tetrads was very low, and the mating of haploid cells from the same ascus was favored because of the physical proximity. Also, mating restriction between haploid cells from the same ascus was found, leading to a very low frequency of self spore clone mating. This mating restriction slowed down the LOH process of the yeast population, maintaining the heterozygote frequency higher than would be expected assuming a fully random mating of the haploid yeasts or according to the Mortimer genome renewal proposal. The observed LOH occurs because of the linkage of the locus MAT to the chromosome III centromere, without the necessity for self spore clone mating or the high frequency of gene conversion and rapid asymmetric LOH observed in genetically unstable yeasts. This phenomenon is enough in itself to explain the high level of homozygosis found in natural populations of wine yeasts. The LOH process for centromere-linked markers would be slower than that for the nonlinked markers, because the linkage decreases the frequency of newly originated heterozygous yeasts after each round of sporulation and mating. This phenomenon is interesting in yeast evolution and may cause important sudden phenotype changes in genetically stable wine yeasts.

show abstract

“…Interestingly, high genetic instability and loss of heterozygosity (LOH) in natural wine yeast strains during laboratory propagation under nonselective conditions have also been observed, but not in the common laboratory strains of S. cerevisiae (Ambrona et al 2005;Ambrona and Ramirez 2007). In contrast to laboratory strains, wine yeast strains do not maintain genetically homogeneous populations because of this propensity to undergo genetic modifications.…”

Section: Rapid Acquisition Of Genomic Modifications In Stressful Envimentioning

confidence: 99%

Noise-Driven Heterogeneity in the Rate of Genetic-Variant Generation as a Basis for Evolvability

Capp

2010

Genetics

View full text Add to dashboard Cite

Molecular biologists have long searched for molecular mechanisms responsible for tuning the rate of genetic-variant generation (RGVG) in fluctuating environments. In spite of several bacterial examples, no regulated variation in the RGVG has been identified in eukaryotic systems. Based notably on the example of industrial and pathogenic yeasts, this article proposes a nonregulated molecular evolutionary mechanism for the appearance of the transient increase of the RGVG in eukaryotic cell populations facing challenging environments. The stochastic nature of gene expression allows a model in which the RGVG in the population can be rapidly tuned as a result of a simple Darwinian process acting on noise-driven heterogeneity in the RGVG from cell to cell. The high flexibility conferred through this model could resolve paradoxical situations, especially concerning the mutator phenotype in cancer cells.

show abstract

Rapid asymmetrical evolution of Saccharomyces cerevisiae wine yeasts

Cited by 17 publications

References 30 publications

Geographic Origin and Diversity of Wine Strains ofSaccharomyces

Geographic Origin and Diversity of Wine Strains ofSaccharomyces

Analysis of Homothallic Saccharomyces cerevisiae Strain Mating during Must Fermentation

Noise-Driven Heterogeneity in the Rate of Genetic-Variant Generation as a Basis for Evolvability

Contact Info

Product

Resources

About