Rapid attachment of Listeria monocytogenes to hydroponic and soil grown lettuce leaves

Kyere, Emmanuel O.; Foong, Grace; Palmer, John W.; Wargent, Jason J.; Fletcher, Graham C.; Flint, Steve

doi:10.1016/j.foodcont.2019.02.015

Cited by 20 publications

(7 citation statements)

References 23 publications

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“…Xylose lysine deoxycholate agar (Difco) and Baird-Parker agar (Difco) were inoculated at 35 °C for 24 h for the enumeration of Salmonella and Staphylococcus aureus , respectively [ 24 ]. Palcam agar was inoculated at 30 °C for 48 h for the enumeration of Listeria monocytogenes [ 25 ]. MacConkey agar supplemented with 2.5 mg/L potassium tellurite solution and 0.05 mg/L cefixime (Land Bridge Technology Co., Ltd., Beijing, China) and was incubated at 37 °C for 24 h for E. coli O157:H7 colony counting [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality

Sun

Zhao

et al. 2022

Foods

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Microbial community distribution in vegetables can affect their quality. This study analyzed the distribution of the microbial community at various stages during processing and storage with the microbial diversity analysis, and evaluated the correlation between the dominant bacteria and sensory quality of lettuce using correspondence analysis with multiple regression analysis. Results showed that the process of washing, cutting, then disinfection and dewatering could change the community distribution and dominant bacteria in lettuce, and maintain better texture, morphology, aroma, color qualities of lettuce. The total number of colonies and relative abundance of Xanthomonas in fresh-cut lettuce decreased, while Afipia and Ralstonia increased during processing and pre-storage (storage for 6 h, 12 h and 1 d). After storage for 3 d, the total number of colonies in lettuce increased (more than 5 log CFU/g), especially the relative abundance of Pseudomonas, which led to the obvious deterioration of the sensory quality of lettuce. Throughout the process, the number of Bacillus cereus, Staphylococcus aureus, and E. coli was less than 100 CFU/g and 3 MPN/g. The number of typical pathogenic bacteria, Salmonella, Listeria monocytogenes and E. coli O157:H7, was below the detection limit. Overall, the prevention and control of psychrotrophic Pseudomonas in lettuce was still necessary. These results will provide useful information for the fresh-cut lettuce industry.

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Section: Methodsmentioning

confidence: 99%

Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality

Sun

Zhao

et al. 2022

Foods

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“…Research from sewage sludge shows the presence of human and animal pathogens such as E. coli, Listeria, Clostridium perfringens, Enterococcus, or Salmonella (Brochier et al, 2012). These pathogens may survive on plant tissues, in soils and in hydroponic systems to which sludge is applied (Brochier et al, 2012;Kyere et al, 2019). Native soil microbial communities are known to decrease the survival of potential pathogens (Xing et al, 2020), but specific strains of bacteria, such as E. coli O104:H4, may survive in soil for over a year after its inoculation (Knödler et al, 2016).…”

Section: Effects Of Dps Application On Soil Microbial Communitiesmentioning

confidence: 99%

Systematic Review of Dairy Processing Sludge and Secondary STRUBIAS Products Used in Agriculture

Khomenko

Shi

et al. 2021

Front. Sustain. Food Syst.

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Worldwide dairy processing plants produce high volumes of dairy processing sludge (DPS), which can be converted into secondary derivatives such as struvite, biochar and ash (collectively termed STRUBIAS). All of these products have high fertilizer equivalent values (FEV), but future certification as phosphorus (P)-fertilizers in the European Union will mean they need to adhere to new technical regulations for fertilizing materials i.e., content limits pertaining to heavy metals (Cd, Cu, Hg, Ni, Pb, and Zn), synthetic organic compounds and pathogens. This systematic review presents the current state of knowledge about these bio-based fertilizers and identifies knowledge gaps. In addition, a review and calculation of greenhouse gas emissions from a range of concept dairy sludge management and production systems for STRUBIAS products [i.e., biochar from pyrolysis and hydrochar from hydrothermal carbonization (HTC)] is presented. Results from the initial review showed that DPS composition depends on product type and treatment processes at a given processing plant, which leads to varied nutrient, heavy metal and carbon contents. These products are all typically high in nutrients and carbon, but low in heavy metals. Further work needs to concentrate on examining their pathogenic microorganism and emerging contaminant contents, in addition to conducting an economic assessment of production and end-user costs related to chemical fertilizer equivalents. With respect to STRUBIAS products, contaminants not present in the raw DPS may need further treatment before being land applied in agriculture e.g., heated producing ashes, hydrochar, or biochar. An examination of these products from an environmental perspective shows that their water quality footprint could be minimized using application rates based on P incorporation of these products into nutrient management planning and application by incorporation into the soil. Results from the concept system showed that elimination of methane emissions was possible, along with a reduction in nitrous oxide. Less carbon (C) is transferred to agricultural fields where DPS is processed into biochar and hydrochar, but due to high recalcitrance, the C in this form is retained much longer in the soil, and therefore STRUBIAS products represent a more stable and long-term option to increase soil C stocks and sequestration.

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“…Listeria spp. are Gram-positive bacteria that are ubiquitous in soil [ 11 ], and they are also found in hydroponic systems [ 12 ], processing environments [ 13 ], and animal operations [ 14 ]. Listeria are found across the supply chain (i.e., from “farm to fork”); thus, tracking this foodborne pathogen is challenging [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Listeria are found across the supply chain (i.e., from “farm to fork”); thus, tracking this foodborne pathogen is challenging [ 15 ]. For instance, Listeria monocytogenes is the bacteria responsible for listeriosis, the third leading cause of death from food poisoning [ 11 , 12 ]. Given the transient nature of Listeria in the food chain, a variety of sensors and biosensors have been developed in the last few decades to monitor pathogens associated with food safety [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Planar Interdigitated Aptasensor for Flow-Through Detection of Listeria spp. in Hydroponic Lettuce Growth Media

Sidhu

Cavallaro

Pola

et al. 2020

Sensors

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Irrigation water is a primary source of fresh produce contamination by bacteria during the preharvest, particularly in hydroponic systems where the control of pests and pathogens is a major challenge. In this work, we demonstrate the development of a Listeria biosensor using platinum interdigitated microelectrodes (Pt-IME). The sensor is incorporated into a particle/sediment trap for the real-time analysis of irrigation water in a hydroponic lettuce system. We demonstrate the application of this system using a smartphone-based potentiostat for rapid on-site analysis of water quality. A detailed characterization of the electrochemical behavior was conducted in the presence/absence of DNA and Listeria spp., which was followed by calibration in various solutions with and without flow. In flow conditions (100 mL samples), the aptasensor had a sensitivity of 3.37 ± 0.21 kΩ log-CFU−1 mL, and the LOD was 48 ± 12 CFU mL−1 with a linear range of 102 to 104 CFU mL−1. In stagnant solution with no flow, the aptasensor performance was significantly improved in buffer, vegetable broth, and hydroponic media. Sensor hysteresis ranged from 2 to 16% after rinsing in a strong basic solution (direct reuse) and was insignificant after removing the aptamer via washing in Piranha solution (reuse after adsorption with fresh aptamer). This is the first demonstration of an aptasensor used to monitor microbial water quality for hydroponic lettuce in real time using a smartphone-based acquisition system for volumes that conform with the regulatory standards. The aptasensor demonstrated a recovery of 90% and may be reused a limited number of times with minor washing steps.

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Rapid attachment of Listeria monocytogenes to hydroponic and soil grown lettuce leaves

Cited by 20 publications

References 23 publications

Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality

Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality

Systematic Review of Dairy Processing Sludge and Secondary STRUBIAS Products Used in Agriculture

Planar Interdigitated Aptasensor for Flow-Through Detection of Listeria spp. in Hydroponic Lettuce Growth Media

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