Rapid Authentication of Turmeric Powder Adulterated With Curcuma Zedoaria and Curcuma Xanthorrhiza Using Ftir-Atr Spectroscopy and Chemometrics

Abstract

Objective: The objective of this study is to develop a rapid, simple, non-destructive and inexpensive analytical method using Fourier Transform Infrared (FTIR) spectroscopy with Attenuated Total Reflection (ATR) as a sampling technique, combined with chemometrics for authentication of turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.
 Methods: Turmeric powder is placed above the diamond crystal in ATR compartment. Spectra are scanned in the absorbance mode from 4000 to 600 cm-1. The obtained spectra is further analyzed by Principal Component Analysis (PCA), Partial Least Square Discriminant Analysis (PLS-DA), and Partial Least Square Regression (PLS-R).
 Results: PCA score plot shows that Curcuma longa, Curcuma zedoaria, and Curcuma xanthorrhiza can be discriminated well. PLS-DA can be used to build the model for classification between pure turmeric powder and adulterated powder with the values of Q2, R2X, and R2Y of 0.9558, 0.9813, and 0.9746, respectively. The good calibration model for quantification of each adulterant is obtained by PLS-R with R2 value more than 0.99 and lower RMSEC value. Both models have been validated by internal and external validation which result in the high R2 value and low RMSEP value which indicates that both models are accurate and precise.
 Conclusion: The combination of FTIR-ATR spectroscopy and chemometrics can be used to authenticate turmeric powder adulterated with Curcuma zedoaria and Curcuma xanthorrhiza.

Formation and Investigation of Physicochemical and Microbiological Properties of Biocomposite Films Containing Turmeric Extract Nano/Microcapsules

“…Characteristic saccharide bands were also present: at wavelengths of 1300, 1092 and 820 cm −1 , they correspond to the C-O stretching bond vibrations, and the peak at 1032 cm −1 shows the CO-C bonds [ 33 ]. The characteristic peaks at about 3400 cm −1 (phenolic O-H stretching vibrations) were observed in composites containing curcumin, 2930 and 2850 cm −1 (methyl (-CH 3 ) and methylene (-CH 2 ) symmetric and asymmetric vibration), 1628 cm −1 (C=C stretching vibration in aromatic molecules), 1597 cm −1 (benzene ring stretching vibrations), 1740 cm −1 (C=O vibrations), 1510 cm −1 (C=C vibrations in the aromatic system), 1428 cm −1 (C-H deformation vibrations), 1278 cm −1 (aromatic C-O stretching vibrations), 1024 cm −1 (C-O-C stretching vibrations) [ 34 , 35 , 36 , 37 ].…”