Rapid, automated, nonradiometric susceptibility testing of Mycobacterium tuberculosis complex to four first-line antituberculous drugs used in standard short-course chemotherapy

Current Perspectives on Drug Susceptibility Testing of Mycobacterium tuberculosis Complex: the Automated Nonradiometric Systems

“…Bacterial contamination from the surrounding environment may occur during DST hands-on procedures and sometimes in one tube only. In this context, a high contamination rate in comparison with B460 has been reported by different authors (16,33,38), who addressed (i) richness of liquid medium and (ii) replacement of rubber septa with screw caps as being mainly responsible.…”

Evaluation of MGIT 960-Based Antimicrobial Testing and Determination of Critical Concentrations of First- and Second-Line Antimicrobial Drugs with Drug-Resistant Clinical Strains of Mycobacterium tuberculosis

“…The reliability of the Becton Dickinson MGIT 960 system for rapid testing of Mycobacterium tuberculosis susceptibility to front-line drugs has been evaluated in several single-and multicenter studies (1,2,4,5,19,21,31,32). However, in most of these studies, only critical concentrations of drugs were tested (1,19,32) and/or limited numbers of drug-resistant and MDR M. tuberculosis strains were included in the analysis (2,4,18,19,21,32).…”

“…However, in most of these studies, only critical concentrations of drugs were tested (1,19,32) and/or limited numbers of drug-resistant and MDR M. tuberculosis strains were included in the analysis (2,4,18,19,21,32). Drug-resistant isolates are likely to exhibit physiological variations in parameters such as growth compared to drug-sensitive strains.…”

Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis

“…Results are available within 8 days (Ardito et al, 2001). Many studies have been performed for evaluating the BACTEC MGIT960 system for DST of M. tuberculosis (Tortoli et al, 2002;Johansen et al, 2004;Scarparo et al, 2004). A recent meta-analysis of the published studies found high accuracy and high predictive values associated with the use of BACTEC MGIT960 (Piersimoni et al, 2006).…”